Standardization of Semi-Quantitative Dot Blotting Assay—Application in the Diagnosis, Follow-Up, and Relapse of Paracoccidioidomycosis

Standardization of Semi-Quantitative Dot Blotting Assay—Application in the Diagnosis, Follow-Up, and Relapse of Paracoccidioidomycosis

Introduction: This study standardized a semi-quantitative dot blotting assay (DB) and a quantitative real-time polymerase chain reaction (qPCR) to detect specific antibodies for <i>Paracoccidioides brasiliensis</i> and its DNA in PCM patients. Methodology: We evaluated 42 confirmed PCM p...

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Bibliographic Details
Main Authors: Beatriz Aparecida Soares Pereira, Ricardo de Souza Cavalcante, Vera Lucia Pereira-Chioccola, Marcia de Souza Carvalho Melhem, Lídia Raquel de Carvalho, Rinaldo Poncio Mendes
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Microorganisms
Subjects:
paracoccidioidomycosis
dot blotting
diagnosis
serology
relapse
Online Access:https://www.mdpi.com/2076-2607/12/2/351

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