Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases
Oligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs and CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation of OGs and CDs attenuates their elicit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2022-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-22-0113-TA |
| _version_ | 1811226913683800064 |
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| author | Anna Scortica Moira Giovannoni Valentina Scafati Francesco Angelucci Felice Cervone Giulia De Lorenzo Manuel Benedetti Benedetta Mattei |
| author_facet | Anna Scortica Moira Giovannoni Valentina Scafati Francesco Angelucci Felice Cervone Giulia De Lorenzo Manuel Benedetti Benedetta Mattei |
| author_sort | Anna Scortica |
| collection | DOAJ |
| description | Oligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs and CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation of OGs and CDs attenuates their elicitor activity and concomitantly releases H2O2. By using a multiple enzyme–based assay, we demonstrate that the H2O2 generated downstream of the combined action between a fungal polygalacturonase and OGOX1 or an endoglucanase and CELLOX can be directed by plant peroxidases (PODs) either towards a reaction possibly involved in plant defense, such as the oxidation of monolignol or a reaction possibly involved in a developmental event, such as the oxidation of auxin (indole-3-acetic acid), pointing to OGOX1 and CELLOX as enzymatic transducers between microbial glycoside hydrolases and plant PODs. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-04-12T09:32:49Z |
| format | Article |
| id | doaj.art-b963aa39ffae46b3acce508d7f6cf778 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T09:32:49Z |
| publishDate | 2022-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-b963aa39ffae46b3acce508d7f6cf7782022-12-22T03:38:19ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062022-10-01351088188610.1094/MPMI-05-22-0113-TABerberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant PeroxidasesAnna Scortica0Moira Giovannoni1Valentina Scafati2Francesco Angelucci3Felice Cervone4Giulia De Lorenzo5Manuel Benedetti6Benedetta Mattei7Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyDepartment of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyDepartment of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyDepartment of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyDepartment of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, ItalyOligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs and CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation of OGs and CDs attenuates their elicitor activity and concomitantly releases H2O2. By using a multiple enzyme–based assay, we demonstrate that the H2O2 generated downstream of the combined action between a fungal polygalacturonase and OGOX1 or an endoglucanase and CELLOX can be directed by plant peroxidases (PODs) either towards a reaction possibly involved in plant defense, such as the oxidation of monolignol or a reaction possibly involved in a developmental event, such as the oxidation of auxin (indole-3-acetic acid), pointing to OGOX1 and CELLOX as enzymatic transducers between microbial glycoside hydrolases and plant PODs. [Graphic: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-22-0113-TAauxinberberine bridge enzyme-like proteinDAMPsglycoside hydrolaseH(2)O(2)lignin |
| spellingShingle | Anna Scortica Moira Giovannoni Valentina Scafati Francesco Angelucci Felice Cervone Giulia De Lorenzo Manuel Benedetti Benedetta Mattei Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases Molecular Plant-Microbe Interactions auxin berberine bridge enzyme-like protein DAMPs glycoside hydrolase H(2)O(2) lignin |
| title | Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases |
| title_full | Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases |
| title_fullStr | Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases |
| title_full_unstemmed | Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases |
| title_short | Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases |
| title_sort | berberine bridge enzyme like oligosaccharide oxidases act as enzymatic transducers between microbial glycoside hydrolases and plant peroxidases |
| topic | auxin berberine bridge enzyme-like protein DAMPs glycoside hydrolase H(2)O(2) lignin |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-22-0113-TA |
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