A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells
A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire col...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618302718 |
| _version_ | 1818727919746285568 |
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| author | Pieter Van den Abbeele Cindy Duysburgh T. Alan Jiang Marco Rebaza Iris Pinheiro Massimo Marzorati |
| author_facet | Pieter Van den Abbeele Cindy Duysburgh T. Alan Jiang Marco Rebaza Iris Pinheiro Massimo Marzorati |
| author_sort | Pieter Van den Abbeele |
| collection | DOAJ |
| description | A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L) in the ascending colon; acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon; propionate (+4.1 mmol/L) in the descending colon. The treatment led to a decrease in ammonium and increased lactate levels in the ascending colon, which correlated with an increase in lactobacilli and bifidobacteria (+2.5 Log/mL). Additionally, the propionate-producing Akkermansia muciniphila increased in the lumen of the proximal colon (up to 7.15 log/mL). Finally, it was observed a protective effect on Caco-2 barrier function in the distal colon and pronounced immune activating properties. The generated data support a possible role in protecting against obesity-related adverse effects. |
| first_indexed | 2024-12-17T22:21:45Z |
| format | Article |
| id | doaj.art-b96baa0c6a094b748f80deaf53c8ec11 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T22:21:45Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-b96baa0c6a094b748f80deaf53c8ec112022-12-21T21:30:28ZengElsevierJournal of Functional Foods1756-46462018-08-0147163171A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cellsPieter Van den Abbeele0Cindy Duysburgh1T. Alan Jiang2Marco Rebaza3Iris Pinheiro4Massimo Marzorati5ProDigest bvba, Technologiepark 3, 9052 Ghent, BelgiumProDigest bvba, Technologiepark 3, 9052 Ghent, BelgiumPlexus Worldwide, 9145 East Pima Center Parkway, Scottsdale, AZ 85258, USAPlexus Worldwide, 9145 East Pima Center Parkway, Scottsdale, AZ 85258, USAProDigest bvba, Technologiepark 3, 9052 Ghent, BelgiumCenter of Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium; Corresponding author.A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L) in the ascending colon; acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon; propionate (+4.1 mmol/L) in the descending colon. The treatment led to a decrease in ammonium and increased lactate levels in the ascending colon, which correlated with an increase in lactobacilli and bifidobacteria (+2.5 Log/mL). Additionally, the propionate-producing Akkermansia muciniphila increased in the lumen of the proximal colon (up to 7.15 log/mL). Finally, it was observed a protective effect on Caco-2 barrier function in the distal colon and pronounced immune activating properties. The generated data support a possible role in protecting against obesity-related adverse effects.http://www.sciencedirect.com/science/article/pii/S1756464618302718XOSPolyphenolsPrebioticSHIMEAkkermansiaObesity |
| spellingShingle | Pieter Van den Abbeele Cindy Duysburgh T. Alan Jiang Marco Rebaza Iris Pinheiro Massimo Marzorati A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells Journal of Functional Foods XOS Polyphenols Prebiotic SHIME Akkermansia Obesity |
| title | A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| title_full | A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| title_fullStr | A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| title_full_unstemmed | A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| title_short | A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| title_sort | combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells |
| topic | XOS Polyphenols Prebiotic SHIME Akkermansia Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618302718 |
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