The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (<i>ASMT</i>)-over...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/572 |
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| author | Hao Wu Xudai Cui Shengyu Guan Guangdong Li Yujun Yao Haixin Wu Jinlong Zhang Xiaosheng Zhang Tuan Yu Yunxiang Li Zhengxing Lian Lu Zhang Guoshi Liu |
| author_facet | Hao Wu Xudai Cui Shengyu Guan Guangdong Li Yujun Yao Haixin Wu Jinlong Zhang Xiaosheng Zhang Tuan Yu Yunxiang Li Zhengxing Lian Lu Zhang Guoshi Liu |
| author_sort | Hao Wu |
| collection | DOAJ |
| description | Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (<i>ASMT</i>)-overexpressed melatonin-enriched dairy goats were successfully generated through the use of pBC1-ASMT expression vector construction and prokaryotic embryo microinjection. Results: These transgenic goats have the same normal phenotype as the wild-type goats (WT). However, the melatonin levels in their blood and milk were significantly increased (<i>p</i> < 0.05). In addition, the quality of their milk was also improved, showing elevated protein content and a reduced somatic cell number compared to the WT goats. No significant changes were detected in the intestinal microbiota patterns between groups. When the animals were challenged by the intravenous injection of <i>E. coli</i>, the <i>ASMT</i>-overexpressed goats had a lower level of pro-inflammatory cytokines and higher anti-inflammatory cytokines compared to the WT goats. Metabolic analysis uncovered a unique arachidonic acid metabolism pattern in transgenic goats. Conclusions: The increased melatonin production due to <i>ASMT</i> overexpression in the transgenic goats may have contributed to their improved milk quality and enhanced the anti-inflammatory ability compared to the WT goats. |
| first_indexed | 2024-03-10T00:49:16Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:49:16Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b96d6acb661241aea2f7ae37c45ba85a2023-11-23T14:53:55ZengMDPI AGMolecules1420-30492022-01-0127257210.3390/molecules27020572The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin ProductionHao Wu0Xudai Cui1Shengyu Guan2Guangdong Li3Yujun Yao4Haixin Wu5Jinlong Zhang6Xiaosheng Zhang7Tuan Yu8Yunxiang Li9Zhengxing Lian10Lu Zhang11Guoshi Liu12National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaQingdao Senmiao Industrial Co., Ltd., Qingdao 266101, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaTianjin Institute of Animal Husbandry and Veterinary, Tianjin 300192, ChinaTianjin Institute of Animal Husbandry and Veterinary, Tianjin 300192, ChinaTianheng Animal Health and Product Quality Supervision Station, Qingdao 266200, ChinaQingdao Senmiao Industrial Co., Ltd., Qingdao 266101, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agricultural, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBackground: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (<i>ASMT</i>)-overexpressed melatonin-enriched dairy goats were successfully generated through the use of pBC1-ASMT expression vector construction and prokaryotic embryo microinjection. Results: These transgenic goats have the same normal phenotype as the wild-type goats (WT). However, the melatonin levels in their blood and milk were significantly increased (<i>p</i> < 0.05). In addition, the quality of their milk was also improved, showing elevated protein content and a reduced somatic cell number compared to the WT goats. No significant changes were detected in the intestinal microbiota patterns between groups. When the animals were challenged by the intravenous injection of <i>E. coli</i>, the <i>ASMT</i>-overexpressed goats had a lower level of pro-inflammatory cytokines and higher anti-inflammatory cytokines compared to the WT goats. Metabolic analysis uncovered a unique arachidonic acid metabolism pattern in transgenic goats. Conclusions: The increased melatonin production due to <i>ASMT</i> overexpression in the transgenic goats may have contributed to their improved milk quality and enhanced the anti-inflammatory ability compared to the WT goats.https://www.mdpi.com/1420-3049/27/2/572acetylserotonin-O-methyltransferase (<i>ASMT</i>)melatoningoatcytokinesintestinal microbiotaarachidonic acid |
| spellingShingle | Hao Wu Xudai Cui Shengyu Guan Guangdong Li Yujun Yao Haixin Wu Jinlong Zhang Xiaosheng Zhang Tuan Yu Yunxiang Li Zhengxing Lian Lu Zhang Guoshi Liu The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production Molecules acetylserotonin-O-methyltransferase (<i>ASMT</i>) melatonin goat cytokines intestinal microbiota arachidonic acid |
| title | The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production |
| title_full | The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production |
| title_fullStr | The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production |
| title_full_unstemmed | The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production |
| title_short | The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (<i>ASMT</i>) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production |
| title_sort | improved milk quality and enhanced anti inflammatory effect in acetylserotonin o methyltransferase i asmt i overexpressed goats an association with the elevated endogenous melatonin production |
| topic | acetylserotonin-O-methyltransferase (<i>ASMT</i>) melatonin goat cytokines intestinal microbiota arachidonic acid |
| url | https://www.mdpi.com/1420-3049/27/2/572 |
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