Gradual Histopathologic Changes During Development of Colorectal Cancer
Background: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-03-01
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| Series: | Iranian Journal of Colorectal Research |
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| Online Access: | https://colorectalresearch.sums.ac.ir/article_47188_b8b0d2034d600892a545207c48126f51.pdf |
| _version_ | 1830285291291672576 |
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| author | Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi |
| author_facet | Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi |
| author_sort | Vahid Nejati |
| collection | DOAJ |
| description | Background: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2 and CD31 changes during colorectal cancer development.Methods: Subcutaneous injections of dimethyl hydrazine DMH were administered to rats (40 mg/kg body weight) for 10 weeks.Rats were fed by food and water until 40th week and sacrificed two by two within 10, 15, 20, 25, 30 and 40 weeks after the start oftreatment. Thin paraffinized sections were applied to anti-CD31, anti-Bcl-2 and anti-p53 staining procedures. MVD and ACF werereported as mean value of three HPFs.Results: Hyperchromatism, TILs and angiogenesis were the most common initial histologic changes which started at 10th week ofDMH treatment. Hyperchromatism’s severity increased earlier than other changes and reached the highest value at the 25th week.The highest value of all variants occurred in the 40th week except the TILs which started to achieve the highest value in week 30 andincreased until 40th week. A diminished amount of p53 was observed at week 40, however, increased intensity of CD31 and Bcl-2were seen between 30th and 40th week.Conclusions: In conclusion, TILs and angiogenesis might be the important earliest factors contributing to colorectal cancer progression. |
| first_indexed | 2024-12-19T03:36:31Z |
| format | Article |
| id | doaj.art-b96f7837a4c24a02804fdeccecce353d |
| institution | Directory Open Access Journal |
| issn | 2783-2430 |
| language | English |
| last_indexed | 2024-12-19T03:36:31Z |
| publishDate | 2019-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Colorectal Research |
| spelling | doaj.art-b96f7837a4c24a02804fdeccecce353d2022-12-21T20:37:22ZengShiraz University of Medical SciencesIranian Journal of Colorectal Research2783-24302019-03-017147188Gradual Histopathologic Changes During Development of Colorectal CancerVahid Nejati0Jamileh Abedi1Maedeh Vakili Saatloo2Maryam Koohsoltani3Rahim Hobbenaghi4Amir Tukmachi5Department of Biology, Faculty of Sciences, Urmia University, Urmia, IranDepartment of Biology, Faculty of Sciences, Urmia University, Urmia, IranDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Urmia University of Medical Sciences, Urmia, IranDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology and Quality Control, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, IranBackground: Colorectal cancer is one of the most common causes of mortality in the world.Objectives: The aim of this study was to investigate the histopathologic changes including hyperchromatism, tissue lymphocyteinfiltrations (TILs), aberrant crypt foci (ACF), microvessel density (MVD), p53, Bcl-2 and CD31 changes during colorectal cancer development.Methods: Subcutaneous injections of dimethyl hydrazine DMH were administered to rats (40 mg/kg body weight) for 10 weeks.Rats were fed by food and water until 40th week and sacrificed two by two within 10, 15, 20, 25, 30 and 40 weeks after the start oftreatment. Thin paraffinized sections were applied to anti-CD31, anti-Bcl-2 and anti-p53 staining procedures. MVD and ACF werereported as mean value of three HPFs.Results: Hyperchromatism, TILs and angiogenesis were the most common initial histologic changes which started at 10th week ofDMH treatment. Hyperchromatism’s severity increased earlier than other changes and reached the highest value at the 25th week.The highest value of all variants occurred in the 40th week except the TILs which started to achieve the highest value in week 30 andincreased until 40th week. A diminished amount of p53 was observed at week 40, however, increased intensity of CD31 and Bcl-2were seen between 30th and 40th week.Conclusions: In conclusion, TILs and angiogenesis might be the important earliest factors contributing to colorectal cancer progression.https://colorectalresearch.sums.ac.ir/article_47188_b8b0d2034d600892a545207c48126f51.pdfcolorectal cancertumor-infiltrating lymphocytes (tils)angiogenesishyperchromatismmicrovessel density (mvd)aberrant crypt foci (acf) |
| spellingShingle | Vahid Nejati Jamileh Abedi Maedeh Vakili Saatloo Maryam Koohsoltani Rahim Hobbenaghi Amir Tukmachi Gradual Histopathologic Changes During Development of Colorectal Cancer Iranian Journal of Colorectal Research colorectal cancer tumor-infiltrating lymphocytes (tils) angiogenesis hyperchromatism microvessel density (mvd) aberrant crypt foci (acf) |
| title | Gradual Histopathologic Changes During Development of Colorectal Cancer |
| title_full | Gradual Histopathologic Changes During Development of Colorectal Cancer |
| title_fullStr | Gradual Histopathologic Changes During Development of Colorectal Cancer |
| title_full_unstemmed | Gradual Histopathologic Changes During Development of Colorectal Cancer |
| title_short | Gradual Histopathologic Changes During Development of Colorectal Cancer |
| title_sort | gradual histopathologic changes during development of colorectal cancer |
| topic | colorectal cancer tumor-infiltrating lymphocytes (tils) angiogenesis hyperchromatism microvessel density (mvd) aberrant crypt foci (acf) |
| url | https://colorectalresearch.sums.ac.ir/article_47188_b8b0d2034d600892a545207c48126f51.pdf |
| work_keys_str_mv | AT vahidnejati gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT jamilehabedi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT maedehvakilisaatloo gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT maryamkoohsoltani gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT rahimhobbenaghi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer AT amirtukmachi gradualhistopathologicchangesduringdevelopmentofcolorectalcancer |