Comparision of structural polypeptides of two field isolates of IBH virus

Inclusion Body Hepatitis (IBH) caused by adenovirus is a reemerging diseaseof mainly young broiler chicks of 3-5 weeks age. The polypeptide pattern of a local isolate of virus causing Inclusion Body Hepatitis was analyzed by sodium dodecyl slulphate-polyacrylamide gel electrophoresis. A total of 9 p...

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Bibliographic Details
Main Authors: R. Kumar, R. Chandra, S. K. Saxena, D. Kumar
Format: Article
Language:English
Published: Veterinary World 2009-01-01
Series:Veterinary World
Subjects:
Online Access:http://www.scopemed.org/fulltextpdf.php?mno=30753
Description
Summary:Inclusion Body Hepatitis (IBH) caused by adenovirus is a reemerging diseaseof mainly young broiler chicks of 3-5 weeks age. The polypeptide pattern of a local isolate of virus causing Inclusion Body Hepatitis was analyzed by sodium dodecyl slulphate-polyacrylamide gel electrophoresis. A total of 9 polypeptides ranging in molecular weight between 35.2 and 110.0 kDa were observed. Western blot analysis of structural polypeptides also revealed that all 9 polypeptides were immunogenic. Out of these 9 polypeptides, 2 viz 97.4 kDa and 53.9 kDa were found to be major immunogenic polypeptide based on intense staining. [Vet World 2009; 2(8.000): 301-303]
ISSN:0972-8988
2231-0916