Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle
The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohist...
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MDPI AG
2021-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/2/192 |
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| author | Charlie Colin-Pierre Valérie Untereiner Ganesh D. Sockalingum Nicolas Berthélémy Louis Danoux Vincent Bardey Solène Mine Christine Jeanmaire Laurent Ramont Stéphane Brézillon |
| author_facet | Charlie Colin-Pierre Valérie Untereiner Ganesh D. Sockalingum Nicolas Berthélémy Louis Danoux Vincent Bardey Solène Mine Christine Jeanmaire Laurent Ramont Stéphane Brézillon |
| author_sort | Charlie Colin-Pierre |
| collection | DOAJ |
| description | The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia. |
| first_indexed | 2024-03-09T03:17:15Z |
| format | Article |
| id | doaj.art-b974d153f5834b3e858ac3db78664e44 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T03:17:15Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b974d153f5834b3e858ac3db78664e442023-12-03T15:17:56ZengMDPI AGBiomolecules2218-273X2021-01-0111219210.3390/biom11020192Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth CycleCharlie Colin-Pierre0Valérie Untereiner1Ganesh D. Sockalingum2Nicolas Berthélémy3Louis Danoux4Vincent Bardey5Solène Mine6Christine Jeanmaire7Laurent Ramont8Stéphane Brézillon9Université de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, 51097 Reims, FranceUniversité de Reims Champagne-Ardenne, PICT, 51097 Reims, FranceUniversité de Reims Champagne-Ardenne, BioSpecT EA7506, UFR de Pharmacie, 51097 Reims, FranceBASF Beauty Care Solutions France SAS, 54425 Pulnoy, FranceBASF Beauty Care Solutions France SAS, 54425 Pulnoy, FranceBASF Beauty Care Solutions France SAS, 54425 Pulnoy, FranceBASF Beauty Care Solutions France SAS, 54425 Pulnoy, FranceBASF Beauty Care Solutions France SAS, 54425 Pulnoy, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, 51097 Reims, FranceUniversité de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, 51097 Reims, FranceThe expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia.https://www.mdpi.com/2218-273X/11/2/192hair follicle growthglycosaminoglycansinfrared spectral imaging<i>k</i>-means clusteringimmunohistochemistry |
| spellingShingle | Charlie Colin-Pierre Valérie Untereiner Ganesh D. Sockalingum Nicolas Berthélémy Louis Danoux Vincent Bardey Solène Mine Christine Jeanmaire Laurent Ramont Stéphane Brézillon Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle Biomolecules hair follicle growth glycosaminoglycans infrared spectral imaging <i>k</i>-means clustering immunohistochemistry |
| title | Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle |
| title_full | Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle |
| title_fullStr | Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle |
| title_full_unstemmed | Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle |
| title_short | Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle |
| title_sort | hair histology and glycosaminoglycans distribution probed by infrared spectral imaging focus on heparan sulfate proteoglycan and glypican 1 during hair growth cycle |
| topic | hair follicle growth glycosaminoglycans infrared spectral imaging <i>k</i>-means clustering immunohistochemistry |
| url | https://www.mdpi.com/2218-273X/11/2/192 |
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