Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops
Rapeseed (<i>Brassica napus</i> L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/11/1788 |
| _version_ | 1797547848614543360 |
|---|---|
| author | Alejandro Jiménez-Gómez Zaki Saati-Santamaría Martin Kostovcik Raúl Rivas Encarna Velázquez Pedro F. Mateos Esther Menéndez Paula García-Fraile |
| author_facet | Alejandro Jiménez-Gómez Zaki Saati-Santamaría Martin Kostovcik Raúl Rivas Encarna Velázquez Pedro F. Mateos Esther Menéndez Paula García-Fraile |
| author_sort | Alejandro Jiménez-Gómez |
| collection | DOAJ |
| description | Rapeseed (<i>Brassica napus</i> L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but efficient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an efficient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain <i>Pseudomonas brassicacearum</i> CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, <i>Pseudomonas brassicacearum</i> CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions. |
| first_indexed | 2024-03-10T14:50:04Z |
| format | Article |
| id | doaj.art-b975ef708c1f4638950c66d5e7ec4bbe |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T14:50:04Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b975ef708c1f4638950c66d5e7ec4bbe2023-11-20T21:03:44ZengMDPI AGAgronomy2073-43952020-11-011011178810.3390/agronomy10111788Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. CropsAlejandro Jiménez-Gómez0Zaki Saati-Santamaría1Martin Kostovcik2Raúl Rivas3Encarna Velázquez4Pedro F. Mateos5Esther Menéndez6Paula García-Fraile7Microbiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainDepartment of Genetics and Microbiology, Faculty of Science, Charles University, 12844 Prague, Czech RepublicMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainMicrobiology and Genetics Department, University of Salamanca, 37007 Salamanca, SpainRapeseed (<i>Brassica napus</i> L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but efficient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an efficient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain <i>Pseudomonas brassicacearum</i> CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, <i>Pseudomonas brassicacearum</i> CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions.https://www.mdpi.com/2073-4395/10/11/1788<i>Pseudomonas</i>PGPBbioinoculantsendophytesbacterial microbiomeculturome |
| spellingShingle | Alejandro Jiménez-Gómez Zaki Saati-Santamaría Martin Kostovcik Raúl Rivas Encarna Velázquez Pedro F. Mateos Esther Menéndez Paula García-Fraile Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops Agronomy <i>Pseudomonas</i> PGPB bioinoculants endophytes bacterial microbiome culturome |
| title | Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops |
| title_full | Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops |
| title_fullStr | Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops |
| title_full_unstemmed | Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops |
| title_short | Selection of the Root Endophyte <i>Pseudomonas brassicacearum</i> CDVBN10 as Plant Growth Promoter for <i>Brassica napus</i> L. Crops |
| title_sort | selection of the root endophyte i pseudomonas brassicacearum i cdvbn10 as plant growth promoter for i brassica napus i l crops |
| topic | <i>Pseudomonas</i> PGPB bioinoculants endophytes bacterial microbiome culturome |
| url | https://www.mdpi.com/2073-4395/10/11/1788 |
| work_keys_str_mv | AT alejandrojimenezgomez selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT zakisaatisantamaria selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT martinkostovcik selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT raulrivas selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT encarnavelazquez selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT pedrofmateos selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT esthermenendez selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops AT paulagarciafraile selectionoftherootendophyteipseudomonasbrassicacearumicdvbn10asplantgrowthpromoterforibrassicanapusilcrops |