An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments
In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore migh...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine and Serum Research Institute
2013-12-01
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| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | http://www.archrazi.com/browse.php?a_id=414&sid=1&slc_lang=en |
| _version_ | 1818230083142287360 |
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| author | Karimnasab, N., Tadayon, K., Khaki, P. Moradi Bidhendi, S. Ghaderi, R. Sekhavati, M. Asadi, F. |
| author_facet | Karimnasab, N., Tadayon, K., Khaki, P. Moradi Bidhendi, S. Ghaderi, R. Sekhavati, M. Asadi, F. |
| author_sort | Karimnasab, N., |
| collection | DOAJ |
| description | In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treated templates and purified DNAs prepared by phenol-chloroform-isoamylalcohol method as well as a commercial extraction kit were subjected to the Salmonella enterica Enteritidis specific STM2 PCR, with exception of crude cell extract, PCR products from all other three methods saved their integrity for 28 days post-generation. This work aimed to find out whether improvement to boiling method can guaranty stability of PCR products. As results showed, treatment of crude cell extracts from S. Enteritidis with proteinase K offers an inexpensive, fast and effective DNA extraction method suitable for high-throughput laboratories. |
| first_indexed | 2024-12-12T10:28:51Z |
| format | Article |
| id | doaj.art-b9773ec8989d4990b18df11155ba4158 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-12T10:28:51Z |
| publishDate | 2013-12-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-b9773ec8989d4990b18df11155ba41582022-12-22T00:27:24ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722013-12-01682105109An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experimentsKarimnasab, N.,Tadayon, K.,Khaki, P.Moradi Bidhendi, S.Ghaderi, R.Sekhavati, M.Asadi, F.In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treated templates and purified DNAs prepared by phenol-chloroform-isoamylalcohol method as well as a commercial extraction kit were subjected to the Salmonella enterica Enteritidis specific STM2 PCR, with exception of crude cell extract, PCR products from all other three methods saved their integrity for 28 days post-generation. This work aimed to find out whether improvement to boiling method can guaranty stability of PCR products. As results showed, treatment of crude cell extracts from S. Enteritidis with proteinase K offers an inexpensive, fast and effective DNA extraction method suitable for high-throughput laboratories.http://www.archrazi.com/browse.php?a_id=414&sid=1&slc_lang=enDNA ExrtactionSalmonella enterica EnteritidisSTM2DNaseproteinase K |
| spellingShingle | Karimnasab, N., Tadayon, K., Khaki, P. Moradi Bidhendi, S. Ghaderi, R. Sekhavati, M. Asadi, F. An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments Archives of Razi Institute DNA Exrtaction Salmonella enterica Enteritidis STM2 DNase proteinase K |
| title | An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments |
| title_full | An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments |
| title_fullStr | An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments |
| title_full_unstemmed | An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments |
| title_short | An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments |
| title_sort | optimized affordable dna extraction method from salmonella enterica enteritidis for pcr experiments |
| topic | DNA Exrtaction Salmonella enterica Enteritidis STM2 DNase proteinase K |
| url | http://www.archrazi.com/browse.php?a_id=414&sid=1&slc_lang=en |
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