Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation
Background: A growing body of evidence demonstrated an immune etiology as well as nonimmune mechanismsfor episodes of clinical acute rejection and long-term allograft dysfunction.Objective: To investigate the correlation of IFN-γ-producing cells and TGF-β with incidence of clinical acuterejection in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2013-04-01
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| Series: | International Journal of Organ Transplantation Medicine |
| Subjects: | |
| Online Access: | http://home.sums.ac.ir/~habibzaf/ojs/index.php/IJOTM/article/view/157/268 |
| _version_ | 1818514166432923648 |
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| author | F. Mohammadi1 M. H. Niknam1 M. Nafar2 B. Einollahi3 B. Nazari1 M. Lessanpezeshki4 M. A. Amirzargar5 G. Solgi5 B. Nikbin1 A. A. Amirzargar1 |
| author_facet | F. Mohammadi1 M. H. Niknam1 M. Nafar2 B. Einollahi3 B. Nazari1 M. Lessanpezeshki4 M. A. Amirzargar5 G. Solgi5 B. Nikbin1 A. A. Amirzargar1 |
| author_sort | F. Mohammadi1 |
| collection | DOAJ |
| description | Background: A growing body of evidence demonstrated an immune etiology as well as nonimmune mechanismsfor episodes of clinical acute rejection and long-term allograft dysfunction.Objective: To investigate the correlation of IFN-γ-producing cells and TGF-β with incidence of clinical acuterejection in living-related and unrelated kidney allogarft recipients during the first post-transplant year.Methods: This multi-center study was performed on 57 kidney allograft recipients from living-related(n=20) and unrelated (n=37) donors between April 2011 and September 2012 and who were followedprospectively for a mean period of one year. Peripheral blood samples were collected from all patientspre-transplantation and at days 14, 30 and 90 after transplantation; PBMCs were used as responding cellsin enzyme-linked immunosorbent spot (ELISPOT) assay to measure the frequency of IFN-γ-producingcells after stimulation with donor lymphocytes. Additionally, TGF-β levels were measured in cell culturesupernatants of ELISPOT assay.Results: During the follow-up period, 45 (79%) patients were diagnosed with stable graft function (groupA); 12 (21%) experienced clinical acute rejection episodes (group B). The frequency of IFN-γ-producingcells was significantly (p<0.001) higher in the rejection group in all three times after transplantation.Also, post-transplantation comparison for TGF-β showed a significantly (p<0.001) higher contents ingroup A vs. group B. Comparing the post-transplantation levels of TGF-β and mean numbers of IFN-γ-producing cells between groups A and B demonstrated a continuous increment in TGF-β and decreasingfrequencies of IFN-γ-producing cells in group A vs. group B.Conclusion: Serial post-transplantation monitoring of IFN-γ-producing donor reactive cells during the firstmonths is a clinically feasible approach for identification of kidney allogarft recipients at risk for ongoingimmune-mediated graft damage and later graft loss. |
| first_indexed | 2024-12-11T00:11:57Z |
| format | Article |
| id | doaj.art-b9781ffe20414fc186594fc41d7c9fc9 |
| institution | Directory Open Access Journal |
| issn | 2008-6490 2008-6482 |
| language | English |
| last_indexed | 2024-12-11T00:11:57Z |
| publishDate | 2013-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Organ Transplantation Medicine |
| spelling | doaj.art-b9781ffe20414fc186594fc41d7c9fc92022-12-22T01:28:06ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64902008-64822013-04-01427785Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal TransplantationF. Mohammadi1M. H. Niknam1M. Nafar2B. Einollahi3B. Nazari1M. Lessanpezeshki4M. A. Amirzargar5G. Solgi5B. Nikbin1A. A. Amirzargar1Background: A growing body of evidence demonstrated an immune etiology as well as nonimmune mechanismsfor episodes of clinical acute rejection and long-term allograft dysfunction.Objective: To investigate the correlation of IFN-γ-producing cells and TGF-β with incidence of clinical acuterejection in living-related and unrelated kidney allogarft recipients during the first post-transplant year.Methods: This multi-center study was performed on 57 kidney allograft recipients from living-related(n=20) and unrelated (n=37) donors between April 2011 and September 2012 and who were followedprospectively for a mean period of one year. Peripheral blood samples were collected from all patientspre-transplantation and at days 14, 30 and 90 after transplantation; PBMCs were used as responding cellsin enzyme-linked immunosorbent spot (ELISPOT) assay to measure the frequency of IFN-γ-producingcells after stimulation with donor lymphocytes. Additionally, TGF-β levels were measured in cell culturesupernatants of ELISPOT assay.Results: During the follow-up period, 45 (79%) patients were diagnosed with stable graft function (groupA); 12 (21%) experienced clinical acute rejection episodes (group B). The frequency of IFN-γ-producingcells was significantly (p<0.001) higher in the rejection group in all three times after transplantation.Also, post-transplantation comparison for TGF-β showed a significantly (p<0.001) higher contents ingroup A vs. group B. Comparing the post-transplantation levels of TGF-β and mean numbers of IFN-γ-producing cells between groups A and B demonstrated a continuous increment in TGF-β and decreasingfrequencies of IFN-γ-producing cells in group A vs. group B.Conclusion: Serial post-transplantation monitoring of IFN-γ-producing donor reactive cells during the firstmonths is a clinically feasible approach for identification of kidney allogarft recipients at risk for ongoingimmune-mediated graft damage and later graft loss.http://home.sums.ac.ir/~habibzaf/ojs/index.php/IJOTM/article/view/157/268KidneyAllograftTGF-βIFN-γ |
| spellingShingle | F. Mohammadi1 M. H. Niknam1 M. Nafar2 B. Einollahi3 B. Nazari1 M. Lessanpezeshki4 M. A. Amirzargar5 G. Solgi5 B. Nikbin1 A. A. Amirzargar1 Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation International Journal of Organ Transplantation Medicine Kidney Allograft TGF-β IFN-γ |
| title | Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation |
| title_full | Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation |
| title_fullStr | Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation |
| title_full_unstemmed | Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation |
| title_short | Dynamic Changes of IFN-γ-producing Cells, TGF-β and Their Predictive Value in Early Outcomes of Renal Transplantation |
| title_sort | dynamic changes of ifn γ producing cells tgf β and their predictive value in early outcomes of renal transplantation |
| topic | Kidney Allograft TGF-β IFN-γ |
| url | http://home.sums.ac.ir/~habibzaf/ojs/index.php/IJOTM/article/view/157/268 |
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