A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters
ABSTRACT Over the years, there has been a lot of emphasis on the development of high-throughput platforms that help identify transporters of drugs and xenobiotics. However, major hinderances in these approaches include substrate promiscuity and functional redundancy of membrane transporters. To tack...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-06-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mbio.00955-22 |
| _version_ | 1811342730962403328 |
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| author | Rajendra Prasad Atanu Banerjee |
| author_facet | Rajendra Prasad Atanu Banerjee |
| author_sort | Rajendra Prasad |
| collection | DOAJ |
| description | ABSTRACT Over the years, there has been a lot of emphasis on the development of high-throughput platforms that help identify transporters of drugs and xenobiotics. However, major hinderances in these approaches include substrate promiscuity and functional redundancy of membrane transporters. To tackle such issues, Almeida and colleagues (L. D. Almeida, A. S. F. Silva, D. C. Mota, A. A. Vasconcelos, et al., mBio 12(6):e03221-21, 2021) elegantly used the power of yeast genetics and created a double gene deletion library for 122 nonessential plasma membrane transporters that facilitates high-throughput identification of drug/xenobiotic transporters. While examining a library of cytotoxic compounds, the authors identified a strong correlation between the chemical structure of azoles and possible import/export routes. Interestingly, the authors also identified the myo-inositol transporter Itr1 to be responsible for import of triazole and imidazole antifungal compounds and proposed a role for the ABC transporter Pdr5 in carbendazim uptake. |
| first_indexed | 2024-04-13T19:15:54Z |
| format | Article |
| id | doaj.art-b97b7d49eea24e83becbbee0c2235b79 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-04-13T19:15:54Z |
| publishDate | 2022-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-b97b7d49eea24e83becbbee0c2235b792022-12-22T02:33:41ZengAmerican Society for MicrobiologymBio2150-75112022-06-0113310.1128/mbio.00955-22A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane TransportersRajendra Prasad0Atanu Banerjee1Amity Institute of Biotechnology, Amity University Haryana, Gurugram, IndiaAmity Institute of Biotechnology, Amity University Haryana, Gurugram, IndiaABSTRACT Over the years, there has been a lot of emphasis on the development of high-throughput platforms that help identify transporters of drugs and xenobiotics. However, major hinderances in these approaches include substrate promiscuity and functional redundancy of membrane transporters. To tackle such issues, Almeida and colleagues (L. D. Almeida, A. S. F. Silva, D. C. Mota, A. A. Vasconcelos, et al., mBio 12(6):e03221-21, 2021) elegantly used the power of yeast genetics and created a double gene deletion library for 122 nonessential plasma membrane transporters that facilitates high-throughput identification of drug/xenobiotic transporters. While examining a library of cytotoxic compounds, the authors identified a strong correlation between the chemical structure of azoles and possible import/export routes. Interestingly, the authors also identified the myo-inositol transporter Itr1 to be responsible for import of triazole and imidazole antifungal compounds and proposed a role for the ABC transporter Pdr5 in carbendazim uptake.https://journals.asm.org/doi/10.1128/mbio.00955-22chemogenomicsSaccharomyces cerevisiaedouble transporter gene deletion librarydrug/xenobiotic transportershigh-throughput screening |
| spellingShingle | Rajendra Prasad Atanu Banerjee A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters mBio chemogenomics Saccharomyces cerevisiae double transporter gene deletion library drug/xenobiotic transporters high-throughput screening |
| title | A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters |
| title_full | A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters |
| title_fullStr | A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters |
| title_full_unstemmed | A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters |
| title_short | A Chemogenomic Toolkit to Evaluate the “Ins and Outs” of Yeast Plasma Membrane Transporters |
| title_sort | chemogenomic toolkit to evaluate the ins and outs of yeast plasma membrane transporters |
| topic | chemogenomics Saccharomyces cerevisiae double transporter gene deletion library drug/xenobiotic transporters high-throughput screening |
| url | https://journals.asm.org/doi/10.1128/mbio.00955-22 |
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