Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology
Undesired maturation and reproduction are major challenges in fish aquaculture regarding genetic introgression, precocious maturation, and reproduction-related mortality, which can have profound ecological or economic impacts. Farming reproductively sterile fish can effectively mitigate these challe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/11/12/2208 |
| _version_ | 1797380501777940480 |
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| author | Lan Xu Mingli Zhao Yonathan Zohar Ten-Tsao Wong |
| author_facet | Lan Xu Mingli Zhao Yonathan Zohar Ten-Tsao Wong |
| author_sort | Lan Xu |
| collection | DOAJ |
| description | Undesired maturation and reproduction are major challenges in fish aquaculture regarding genetic introgression, precocious maturation, and reproduction-related mortality, which can have profound ecological or economic impacts. Farming reproductively sterile fish can effectively mitigate these challenges. In this paper, we transferred and applied a novel immersion-based, non-transgenic gene silencing technology to sterilize coho salmon for the first time. Unfertilized eggs were bath immersion-treated with <i>csdnd</i>-MO-Vivo in different immersion media. Eyed rates of treated groups ranged from 0.9 to 63.5%. Sterile fish lacking germ cells, and those with arrested germ cells/atretic oocytes, were obtained at 14 and 20 months of age, albeit at a low percentage (2.3 to 10.0% based on females). Gonadal histology and <i>vasa</i>/<i>nanos3</i> gene expression profile were provided for comparing fertile and sterile gonads, as well as retarded ovaries. Future directions and strategies for optimizing the technology and improving sterility induction were also proposed. The successful production of sterile coho salmon achieved in this study demonstrates the proof of principle for this new sterilization technology. As we continue to expand upon these findings and refine the technology, achieving coho salmon sterile population farming would facilitate the future transfer and application to other commercially important aquaculture fish. |
| first_indexed | 2024-03-08T20:38:13Z |
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| id | doaj.art-b97ea810e21044c18b48e48e8d1a9858 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-08T20:38:13Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-b97ea810e21044c18b48e48e8d1a98582023-12-22T14:18:33ZengMDPI AGJournal of Marine Science and Engineering2077-13122023-11-011112220810.3390/jmse11122208Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing TechnologyLan Xu0Mingli Zhao1Yonathan Zohar2Ten-Tsao Wong3Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USAInstitute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USADepartment of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USADepartment of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USAUndesired maturation and reproduction are major challenges in fish aquaculture regarding genetic introgression, precocious maturation, and reproduction-related mortality, which can have profound ecological or economic impacts. Farming reproductively sterile fish can effectively mitigate these challenges. In this paper, we transferred and applied a novel immersion-based, non-transgenic gene silencing technology to sterilize coho salmon for the first time. Unfertilized eggs were bath immersion-treated with <i>csdnd</i>-MO-Vivo in different immersion media. Eyed rates of treated groups ranged from 0.9 to 63.5%. Sterile fish lacking germ cells, and those with arrested germ cells/atretic oocytes, were obtained at 14 and 20 months of age, albeit at a low percentage (2.3 to 10.0% based on females). Gonadal histology and <i>vasa</i>/<i>nanos3</i> gene expression profile were provided for comparing fertile and sterile gonads, as well as retarded ovaries. Future directions and strategies for optimizing the technology and improving sterility induction were also proposed. The successful production of sterile coho salmon achieved in this study demonstrates the proof of principle for this new sterilization technology. As we continue to expand upon these findings and refine the technology, achieving coho salmon sterile population farming would facilitate the future transfer and application to other commercially important aquaculture fish.https://www.mdpi.com/2077-1312/11/12/2208genetic containmentprecocious maturationcoho salmonPGC<i>dnd</i>-Morpholino-Vivoreproductively sterile fish |
| spellingShingle | Lan Xu Mingli Zhao Yonathan Zohar Ten-Tsao Wong Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology Journal of Marine Science and Engineering genetic containment precocious maturation coho salmon PGC <i>dnd</i>-Morpholino-Vivo reproductively sterile fish |
| title | Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology |
| title_full | Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology |
| title_fullStr | Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology |
| title_full_unstemmed | Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology |
| title_short | Induction of Reproductive Sterility in Coho Salmon (<i>Oncorhynchus kisutch</i>) by an Immersion-Based Gene Silencing Technology |
| title_sort | induction of reproductive sterility in coho salmon i oncorhynchus kisutch i by an immersion based gene silencing technology |
| topic | genetic containment precocious maturation coho salmon PGC <i>dnd</i>-Morpholino-Vivo reproductively sterile fish |
| url | https://www.mdpi.com/2077-1312/11/12/2208 |
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