Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari
Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metab...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2014-06-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300003&lng=en&tlng=en |
| _version_ | 1828155572460978176 |
|---|---|
| author | Plinio Rodrigues dos Santos Filho Breno Régis Santos Sandro Barbosa Letícia Rios Vieira Natália Chagas de Freitas Daniele Ferreira Dias Marcelo Henrique dos Santos |
| author_facet | Plinio Rodrigues dos Santos Filho Breno Régis Santos Sandro Barbosa Letícia Rios Vieira Natália Chagas de Freitas Daniele Ferreira Dias Marcelo Henrique dos Santos |
| author_sort | Plinio Rodrigues dos Santos Filho |
| collection | DOAJ |
| description | Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metabolites in this plant. Thus, the aim of this work was to determine the growth curve and to perform the biochemical and phytochemical analyses in calli obtained from the procambium segments of Bacupari. The growth curve of calli followed a sigmoidal pattern, with four distinct phases (lag, exponential, linear, deceleration). Total soluble sugars were higher on the inoculation day and the reducing sugars on the 20 th day. Amino acids increased from the 60 th day up to the stabilization on the 120 th day. The protein content varied, but it seemed to be related to the amino acids metabolism. The phytochemical screening showed the presence of phenolic and flavonoid compounds in the calli and the HPLC analysis allowed the identification of Fukugetin, Guttiferone A and 7-epiclusianone. |
| first_indexed | 2024-04-11T22:55:43Z |
| format | Article |
| id | doaj.art-b980cba90f084f1cb6514da74a4b9af8 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T22:55:43Z |
| publishDate | 2014-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-b980cba90f084f1cb6514da74a4b9af82022-12-22T03:58:25ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242014-06-0157332633310.1590/S1516-89132014000300003S1516-89132014000300003Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of BacupariPlinio Rodrigues dos Santos Filho0Breno Régis Santos1Sandro Barbosa2Letícia Rios Vieira3Natália Chagas de Freitas4Daniele Ferreira Dias5Marcelo Henrique dos Santos6Universidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de AlfenasUniversidade Federal de ViçosaGarcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metabolites in this plant. Thus, the aim of this work was to determine the growth curve and to perform the biochemical and phytochemical analyses in calli obtained from the procambium segments of Bacupari. The growth curve of calli followed a sigmoidal pattern, with four distinct phases (lag, exponential, linear, deceleration). Total soluble sugars were higher on the inoculation day and the reducing sugars on the 20 th day. Amino acids increased from the 60 th day up to the stabilization on the 120 th day. The protein content varied, but it seemed to be related to the amino acids metabolism. The phytochemical screening showed the presence of phenolic and flavonoid compounds in the calli and the HPLC analysis allowed the identification of Fukugetin, Guttiferone A and 7-epiclusianone.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300003&lng=en&tlng=enBacuparibenzophenonescalli |
| spellingShingle | Plinio Rodrigues dos Santos Filho Breno Régis Santos Sandro Barbosa Letícia Rios Vieira Natália Chagas de Freitas Daniele Ferreira Dias Marcelo Henrique dos Santos Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari Brazilian Archives of Biology and Technology Bacupari benzophenones calli |
| title | Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari |
| title_full | Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari |
| title_fullStr | Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari |
| title_full_unstemmed | Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari |
| title_short | Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari |
| title_sort | growth curve biochemical profile and phytochemical analyses in calli obtained from the procambium segments of bacupari |
| topic | Bacupari benzophenones calli |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300003&lng=en&tlng=en |
| work_keys_str_mv | AT pliniorodriguesdossantosfilho growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT brenoregissantos growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT sandrobarbosa growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT leticiariosvieira growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT nataliachagasdefreitas growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT danieleferreiradias growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari AT marcelohenriquedossantos growthcurvebiochemicalprofileandphytochemicalanalysesincalliobtainedfromtheprocambiumsegmentsofbacupari |