Clinical and Biological Significances of FBLN5 in Gastric Cancer
Abnormal FBLN5 expression levels are related to various cancer types. This study is the first to explore its clinical and biological significances in gastric cancer (GC). We used The Cancer Genome Atlas-GC (TCGA-GC) and Gene Expression Omnibus (GEO) databases to identify the differential expression...
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| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/2/553 |
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| author | Xiulan Bian Shengjie Yin Xin Yin Tianyi Fang Yufei Wang Shuo Yang Xinju Jiang Yingwei Xue Fei Ye Lei Zhang |
| author_facet | Xiulan Bian Shengjie Yin Xin Yin Tianyi Fang Yufei Wang Shuo Yang Xinju Jiang Yingwei Xue Fei Ye Lei Zhang |
| author_sort | Xiulan Bian |
| collection | DOAJ |
| description | Abnormal FBLN5 expression levels are related to various cancer types. This study is the first to explore its clinical and biological significances in gastric cancer (GC). We used The Cancer Genome Atlas-GC (TCGA-GC) and Gene Expression Omnibus (GEO) databases to identify the differential expression of FBLN5, and its association with clinical pathological characteristics was analyzed. A Kaplan–Meier plotter was used to calculate the impact of FBLN5 on GC patient prognosis, and the biological functions of FBLN5 were analyzed. In addition, we constructed a GC tissue microarray, and performed an immunohistochemical staining of FBLN5 to verify our findings. Western blotting was conducted simultaneously to confirm that FBLN5 was overexpressed in GC. We found that the high level of FBLN5 mRNA in GC was associated with a poor prognosis. High FBLN5 expression levels were significantly correlated with INFc and N3 lymph node metastasis. Univariate and multivariate analyses showed that FBLN5 expression levels and lymph node metastasis rate were independent risk factors related to GC patient prognosis, which can be combined to construct a nomogram to serve patients. Therefore, we believe that FBLN5 is significantly related to the poor prognosis of GC patients. FBLN5 is a valuable prognostic indicator to evaluate the prognosis of GC. |
| first_indexed | 2024-03-09T13:16:46Z |
| format | Article |
| id | doaj.art-b9847cbb6f5f478ab317ec58f2c83d0b |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T13:16:46Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b9847cbb6f5f478ab317ec58f2c83d0b2023-11-30T21:35:31ZengMDPI AGCancers2072-66942023-01-0115255310.3390/cancers15020553Clinical and Biological Significances of FBLN5 in Gastric CancerXiulan Bian0Shengjie Yin1Xin Yin2Tianyi Fang3Yufei Wang4Shuo Yang5Xinju Jiang6Yingwei Xue7Fei Ye8Lei Zhang9Department of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150086, ChinaDepartment of Medical Oncology, Municipal Hospital of Chifeng, Chifeng 024000, ChinaDepartment of Gastroenterological Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150086, ChinaDepartment of Gastroenterological Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150086, ChinaDepartment of Gastroenterological Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150086, ChinaDepartment of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150086, ChinaDepartment of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150086, ChinaDepartment of Gastroenterological Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150086, ChinaDepartment of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150086, ChinaDepartment of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150086, ChinaAbnormal FBLN5 expression levels are related to various cancer types. This study is the first to explore its clinical and biological significances in gastric cancer (GC). We used The Cancer Genome Atlas-GC (TCGA-GC) and Gene Expression Omnibus (GEO) databases to identify the differential expression of FBLN5, and its association with clinical pathological characteristics was analyzed. A Kaplan–Meier plotter was used to calculate the impact of FBLN5 on GC patient prognosis, and the biological functions of FBLN5 were analyzed. In addition, we constructed a GC tissue microarray, and performed an immunohistochemical staining of FBLN5 to verify our findings. Western blotting was conducted simultaneously to confirm that FBLN5 was overexpressed in GC. We found that the high level of FBLN5 mRNA in GC was associated with a poor prognosis. High FBLN5 expression levels were significantly correlated with INFc and N3 lymph node metastasis. Univariate and multivariate analyses showed that FBLN5 expression levels and lymph node metastasis rate were independent risk factors related to GC patient prognosis, which can be combined to construct a nomogram to serve patients. Therefore, we believe that FBLN5 is significantly related to the poor prognosis of GC patients. FBLN5 is a valuable prognostic indicator to evaluate the prognosis of GC.https://www.mdpi.com/2072-6694/15/2/553gastric cancerFBLN5fibroblastprognosis |
| spellingShingle | Xiulan Bian Shengjie Yin Xin Yin Tianyi Fang Yufei Wang Shuo Yang Xinju Jiang Yingwei Xue Fei Ye Lei Zhang Clinical and Biological Significances of FBLN5 in Gastric Cancer Cancers gastric cancer FBLN5 fibroblast prognosis |
| title | Clinical and Biological Significances of FBLN5 in Gastric Cancer |
| title_full | Clinical and Biological Significances of FBLN5 in Gastric Cancer |
| title_fullStr | Clinical and Biological Significances of FBLN5 in Gastric Cancer |
| title_full_unstemmed | Clinical and Biological Significances of FBLN5 in Gastric Cancer |
| title_short | Clinical and Biological Significances of FBLN5 in Gastric Cancer |
| title_sort | clinical and biological significances of fbln5 in gastric cancer |
| topic | gastric cancer FBLN5 fibroblast prognosis |
| url | https://www.mdpi.com/2072-6694/15/2/553 |
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