The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety
Mood disorders, such as anxiety and depression, are commonly found in people suffering from chronic pain. Serotonin–norepinephrine reuptake inhibitors (SNRIs) are potential in alleviating chronic pain and are the first-line option for anxiety disorder. The anterior cingulate cortex (ACC) plays a vit...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.992130/full |
| _version_ | 1798015680593788928 |
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| author | Chenglin Li Kaiji Ni Meiru Qi Jie Li Kexin Yang Yanli Luo |
| author_facet | Chenglin Li Kaiji Ni Meiru Qi Jie Li Kexin Yang Yanli Luo |
| author_sort | Chenglin Li |
| collection | DOAJ |
| description | Mood disorders, such as anxiety and depression, are commonly found in people suffering from chronic pain. Serotonin–norepinephrine reuptake inhibitors (SNRIs) are potential in alleviating chronic pain and are the first-line option for anxiety disorder. The anterior cingulate cortex (ACC) plays a vital role in chronic pain-induced anxiety, but its role in the therapeutic effects of SNRIs remains largely unclear. We used complete Freund’s adjuvant (CFA) in this current study to induce chronic inflammatory pain. Von Frey test was used to measure the mechanical withdrawal threshold. The elevated plus maze test (EPM) and the novelty-suppressed feeding test (NSF) were used to measure anxiety-like behaviors. Twenty-one days after the modeling, anxiety-like behaviors were successfully induced in CFA mice, and a 3-day intraperitoneal injection of duloxetine attenuated such behaviors. While, mechanical hyperalgesia was also improved. Then, we locally infused duloxetine in ACC for 3 days only to find out its analgesic effect in CFA mice. Furthermore, we used fiber photometry to discover decreased glutamatergic excitability and enhanced serotonin concentration in ACC after intraperitoneal injection of duloxetine. Overall, this study proposed a potential mechanism for the analgesic effect of duloxetine and shed light on further studies on the mechanism of its anxiolytic effect in chronic pain-induced anxiety. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-11T15:38:29Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj.art-b984cf55f64a45fbb45c698745bcc6532022-12-22T04:15:54ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-11-011610.3389/fnins.2022.992130992130The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxietyChenglin Li0Kaiji Ni1Meiru Qi2Jie Li3Kexin Yang4Yanli Luo5Department of Psychological Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Psychological Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Psychological Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaSchool of Life Sciences and Technology, ShanghaiTech University, Shanghai, ChinaDepartment of Psychological Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMood disorders, such as anxiety and depression, are commonly found in people suffering from chronic pain. Serotonin–norepinephrine reuptake inhibitors (SNRIs) are potential in alleviating chronic pain and are the first-line option for anxiety disorder. The anterior cingulate cortex (ACC) plays a vital role in chronic pain-induced anxiety, but its role in the therapeutic effects of SNRIs remains largely unclear. We used complete Freund’s adjuvant (CFA) in this current study to induce chronic inflammatory pain. Von Frey test was used to measure the mechanical withdrawal threshold. The elevated plus maze test (EPM) and the novelty-suppressed feeding test (NSF) were used to measure anxiety-like behaviors. Twenty-one days after the modeling, anxiety-like behaviors were successfully induced in CFA mice, and a 3-day intraperitoneal injection of duloxetine attenuated such behaviors. While, mechanical hyperalgesia was also improved. Then, we locally infused duloxetine in ACC for 3 days only to find out its analgesic effect in CFA mice. Furthermore, we used fiber photometry to discover decreased glutamatergic excitability and enhanced serotonin concentration in ACC after intraperitoneal injection of duloxetine. Overall, this study proposed a potential mechanism for the analgesic effect of duloxetine and shed light on further studies on the mechanism of its anxiolytic effect in chronic pain-induced anxiety.https://www.frontiersin.org/articles/10.3389/fnins.2022.992130/fullduloxetinechronic painanxietyanterior cingulate cortexfiber photometry |
| spellingShingle | Chenglin Li Kaiji Ni Meiru Qi Jie Li Kexin Yang Yanli Luo The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety Frontiers in Neuroscience duloxetine chronic pain anxiety anterior cingulate cortex fiber photometry |
| title | The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety |
| title_full | The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety |
| title_fullStr | The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety |
| title_full_unstemmed | The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety |
| title_short | The anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain-induced anxiety |
| title_sort | anterior cingulate cortex contributes to the analgesic rather than the anxiolytic effects of duloxetine in chronic pain induced anxiety |
| topic | duloxetine chronic pain anxiety anterior cingulate cortex fiber photometry |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.992130/full |
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