Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcripto...
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MDPI AG
2021-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/1/58 |
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| author | Xingwan Yi Huabei Gao Yi Yang Shumin Yang Le Luo Chao Yu Jia Wang Tangren Cheng Qixiang Zhang Huitang Pan |
| author_facet | Xingwan Yi Huabei Gao Yi Yang Shumin Yang Le Luo Chao Yu Jia Wang Tangren Cheng Qixiang Zhang Huitang Pan |
| author_sort | Xingwan Yi |
| collection | DOAJ |
| description | Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcriptome data of once- and continuous-flowering roses in our previous study were used to conduct weighted gene co-expression network analysis (WGCNA) to obtain the candidate genes related to flowering transitions. The expression patterns of candidate genes at different developmental stages between <i>Rosa chinensis</i> “Old Blush” (continuous-flowering cultivar) and <i>R.</i> “Huan Die” (once-flowering cultivar) were investigated, and the relationship of the key gene with the endogenous hormone was analyzed. The results showed that the expression trends of <i>VIN3-LIKE 1</i> (<i>VIL1</i>), <i>FRIGIDA- LIKE 3</i> (<i>FRI3</i>), <i>APETALA 2- LIKE</i> (<i>AP2-like</i>) and <i>CONSTANS-LIKE 2</i> (<i>CO-like 2</i>) genes were significantly different between “Old Blush” and “Huan Die”, and the expression trends of <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1</i> (<i>SOC1</i>) and <i>CO-like 2</i> were consistent in the flowering transition of “Old Blush” under different environments. The changes in cytokinin and gibberellic acid (GA<sub>3</sub>) content were different in the two rose cultivars. The overall change trend of the abscisic acid and GA<sub>3</sub> in the flowering transition of “Old Blush” under different environments was consistent. The promoter sequence of <i>CO-like 2</i> contained a P-box element associated with gibberellin response, as well as binding sites for transcription factors. In a word, we found <i>CO-like 2</i> associated with continuous flowering and some factors that may synergistically regulate continuous flowering. The results provided a reference for elucidating the molecular regulatory mechanisms of continuous-flowering traits in roses. |
| first_indexed | 2024-03-10T01:50:21Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
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| spelling | doaj.art-b986c93858714acbb96b9e770680e1832023-11-23T13:06:24ZengMDPI AGBiomolecules2218-273X2021-12-011215810.3390/biom12010058Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering RosesXingwan Yi0Huabei Gao1Yi Yang2Shumin Yang3Le Luo4Chao Yu5Jia Wang6Tangren Cheng7Qixiang Zhang8Huitang Pan9National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaNational Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaRoses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcriptome data of once- and continuous-flowering roses in our previous study were used to conduct weighted gene co-expression network analysis (WGCNA) to obtain the candidate genes related to flowering transitions. The expression patterns of candidate genes at different developmental stages between <i>Rosa chinensis</i> “Old Blush” (continuous-flowering cultivar) and <i>R.</i> “Huan Die” (once-flowering cultivar) were investigated, and the relationship of the key gene with the endogenous hormone was analyzed. The results showed that the expression trends of <i>VIN3-LIKE 1</i> (<i>VIL1</i>), <i>FRIGIDA- LIKE 3</i> (<i>FRI3</i>), <i>APETALA 2- LIKE</i> (<i>AP2-like</i>) and <i>CONSTANS-LIKE 2</i> (<i>CO-like 2</i>) genes were significantly different between “Old Blush” and “Huan Die”, and the expression trends of <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1</i> (<i>SOC1</i>) and <i>CO-like 2</i> were consistent in the flowering transition of “Old Blush” under different environments. The changes in cytokinin and gibberellic acid (GA<sub>3</sub>) content were different in the two rose cultivars. The overall change trend of the abscisic acid and GA<sub>3</sub> in the flowering transition of “Old Blush” under different environments was consistent. The promoter sequence of <i>CO-like 2</i> contained a P-box element associated with gibberellin response, as well as binding sites for transcription factors. In a word, we found <i>CO-like 2</i> associated with continuous flowering and some factors that may synergistically regulate continuous flowering. The results provided a reference for elucidating the molecular regulatory mechanisms of continuous-flowering traits in roses.https://www.mdpi.com/2218-273X/12/1/58rosecontinuous floweringflowering transitionphytohormoneWGCNA |
| spellingShingle | Xingwan Yi Huabei Gao Yi Yang Shumin Yang Le Luo Chao Yu Jia Wang Tangren Cheng Qixiang Zhang Huitang Pan Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses Biomolecules rose continuous flowering flowering transition phytohormone WGCNA |
| title | Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses |
| title_full | Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses |
| title_fullStr | Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses |
| title_full_unstemmed | Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses |
| title_short | Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses |
| title_sort | differentially expressed genes related to flowering transition between once and continuous flowering roses |
| topic | rose continuous flowering flowering transition phytohormone WGCNA |
| url | https://www.mdpi.com/2218-273X/12/1/58 |
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