A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics

Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.

Bibliographic Details
Main Authors: Pan Fang, Yanlong Ji, Ivan Silbern, Carmen Doebele, Momchil Ninov, Christof Lenz, Thomas Oellerich, Kuan-Ting Pan, Henning Urlaub
Format: Article
Language:English
Published: Nature Portfolio 2020-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19052-w
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author Pan Fang
Yanlong Ji
Ivan Silbern
Carmen Doebele
Momchil Ninov
Christof Lenz
Thomas Oellerich
Kuan-Ting Pan
Henning Urlaub
author_facet Pan Fang
Yanlong Ji
Ivan Silbern
Carmen Doebele
Momchil Ninov
Christof Lenz
Thomas Oellerich
Kuan-Ting Pan
Henning Urlaub
author_sort Pan Fang
collection DOAJ
description Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.
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spelling doaj.art-b98847062cd64d348c1ad9038cab217f2022-12-21T19:12:54ZengNature PortfolioNature Communications2041-17232020-10-0111111110.1038/s41467-020-19052-wA streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomicsPan Fang0Yanlong Ji1Ivan Silbern2Carmen Doebele3Momchil Ninov4Christof Lenz5Thomas Oellerich6Kuan-Ting Pan7Henning Urlaub8Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryComprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.https://doi.org/10.1038/s41467-020-19052-w
spellingShingle Pan Fang
Yanlong Ji
Ivan Silbern
Carmen Doebele
Momchil Ninov
Christof Lenz
Thomas Oellerich
Kuan-Ting Pan
Henning Urlaub
A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
Nature Communications
title A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
title_full A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
title_fullStr A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
title_full_unstemmed A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
title_short A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
title_sort streamlined pipeline for multiplexed quantitative site specific n glycoproteomics
url https://doi.org/10.1038/s41467-020-19052-w
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