A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.
Main Authors: | , , , , , , , , |
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Format: | Article |
Language: | English |
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Nature Portfolio
2020-10-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-19052-w |
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author | Pan Fang Yanlong Ji Ivan Silbern Carmen Doebele Momchil Ninov Christof Lenz Thomas Oellerich Kuan-Ting Pan Henning Urlaub |
author_facet | Pan Fang Yanlong Ji Ivan Silbern Carmen Doebele Momchil Ninov Christof Lenz Thomas Oellerich Kuan-Ting Pan Henning Urlaub |
author_sort | Pan Fang |
collection | DOAJ |
description | Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool. |
first_indexed | 2024-12-21T06:35:23Z |
format | Article |
id | doaj.art-b98847062cd64d348c1ad9038cab217f |
institution | Directory Open Access Journal |
issn | 2041-1723 |
language | English |
last_indexed | 2024-12-21T06:35:23Z |
publishDate | 2020-10-01 |
publisher | Nature Portfolio |
record_format | Article |
series | Nature Communications |
spelling | doaj.art-b98847062cd64d348c1ad9038cab217f2022-12-21T19:12:54ZengNature PortfolioNature Communications2041-17232020-10-0111111110.1038/s41467-020-19052-wA streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomicsPan Fang0Yanlong Ji1Ivan Silbern2Carmen Doebele3Momchil Ninov4Christof Lenz5Thomas Oellerich6Kuan-Ting Pan7Henning Urlaub8Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityHematology/Oncology, Department of Medicine II, Johann Wolfgang Goethe UniversityBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical ChemistryComprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.https://doi.org/10.1038/s41467-020-19052-w |
spellingShingle | Pan Fang Yanlong Ji Ivan Silbern Carmen Doebele Momchil Ninov Christof Lenz Thomas Oellerich Kuan-Ting Pan Henning Urlaub A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics Nature Communications |
title | A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics |
title_full | A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics |
title_fullStr | A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics |
title_full_unstemmed | A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics |
title_short | A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics |
title_sort | streamlined pipeline for multiplexed quantitative site specific n glycoproteomics |
url | https://doi.org/10.1038/s41467-020-19052-w |
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