Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism
IntroductionThe adverse effects of high glucose on embryos can be traced to the preimplantation stage. This study aimed to observe the effect of high glucose on early-stage embryos. Methods and resultsSeven-week-old ICR female mice were superovulated and mated, and the zygotes were collected. The zy...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1135837/full |
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| author | Jiewei Hong Hongxuan Tong Xuan Wang Xiaoyan Lv Lijuan He Xuezhi Yang Yingli Wang Kaixia Xu Qi Liang Qianjin Feng Tingli Niu Xin Niu Yu Lu |
| author_facet | Jiewei Hong Hongxuan Tong Xuan Wang Xiaoyan Lv Lijuan He Xuezhi Yang Yingli Wang Kaixia Xu Qi Liang Qianjin Feng Tingli Niu Xin Niu Yu Lu |
| author_sort | Jiewei Hong |
| collection | DOAJ |
| description | IntroductionThe adverse effects of high glucose on embryos can be traced to the preimplantation stage. This study aimed to observe the effect of high glucose on early-stage embryos. Methods and resultsSeven-week-old ICR female mice were superovulated and mated, and the zygotes were collected. The zygotes were randomly cultured in 5 different glucose concentrations (control, 20mM, 40mM, 60mM and 80mM glucose). The cleavage rate, blastocyst rate and total cell number of blastocyst were used to assess the embryo quality. 40 mM glucose was selected to model high glucose levels in this study. 40mM glucose arrested early embryonic development, and the blastocyst rate and total cell number of the blastocyst decreased significantly as glucose concentration was increased. The reduction in the total cell number of blastocysts in the high glucose group was attributed to decreased proliferation and increased cell apoptosis, which is associated with the diminished expression of GLUTs (GLUT1, GLUT2, GLUT3). Furthermore, the metabolic characterization of blastocyst culture was observed in the high-glucose environment. DiscussionThe balance of glycolysis and oxidative phosphorylation at the blastocyst stage was disrupted. And embryo development arrest due to high glucose is associated with changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism. |
| first_indexed | 2024-03-08T22:29:41Z |
| format | Article |
| id | doaj.art-b988dc530a5549eaa8b9103fd654461f |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-08T22:29:41Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-b988dc530a5549eaa8b9103fd654461f2023-12-18T06:49:02ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-12-011410.3389/fendo.2023.11358371135837Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolismJiewei Hong0Hongxuan Tong1Xuan Wang2Xiaoyan Lv3Lijuan He4Xuezhi Yang5Yingli Wang6Kaixia Xu7Qi Liang8Qianjin Feng9Tingli Niu10Xin Niu11Yu Lu12School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaInstitute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaParty Committee Office, Shanxi Health Vocational College, Shanxi, ChinaLibrary Collection and Editing Department, Beijing University of Chinese Medicine, Beijing, ChinaRehabilitation Department, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaExperimental Management Center, Shanxi University of Traditional Chinese Medicine, Shanxi, ChinaSchool of Basic Medicine, Shanxi University of Traditional Chinese Medicine, Shanxi, ChinaCentre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA, AustraliaExperimental Management Center, Shanxi University of Traditional Chinese Medicine, Shanxi, ChinaMedical Insurance Office, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China0Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaIntroductionThe adverse effects of high glucose on embryos can be traced to the preimplantation stage. This study aimed to observe the effect of high glucose on early-stage embryos. Methods and resultsSeven-week-old ICR female mice were superovulated and mated, and the zygotes were collected. The zygotes were randomly cultured in 5 different glucose concentrations (control, 20mM, 40mM, 60mM and 80mM glucose). The cleavage rate, blastocyst rate and total cell number of blastocyst were used to assess the embryo quality. 40 mM glucose was selected to model high glucose levels in this study. 40mM glucose arrested early embryonic development, and the blastocyst rate and total cell number of the blastocyst decreased significantly as glucose concentration was increased. The reduction in the total cell number of blastocysts in the high glucose group was attributed to decreased proliferation and increased cell apoptosis, which is associated with the diminished expression of GLUTs (GLUT1, GLUT2, GLUT3). Furthermore, the metabolic characterization of blastocyst culture was observed in the high-glucose environment. DiscussionThe balance of glycolysis and oxidative phosphorylation at the blastocyst stage was disrupted. And embryo development arrest due to high glucose is associated with changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism. https://www.frontiersin.org/articles/10.3389/fendo.2023.1135837/fullembryo arresthigh glucoseembryo metabolismin vitroGLUTsGC-MS |
| spellingShingle | Jiewei Hong Hongxuan Tong Xuan Wang Xiaoyan Lv Lijuan He Xuezhi Yang Yingli Wang Kaixia Xu Qi Liang Qianjin Feng Tingli Niu Xin Niu Yu Lu Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism Frontiers in Endocrinology embryo arrest high glucose embryo metabolism in vitro GLUTs GC-MS |
| title | Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism |
| title_full | Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism |
| title_fullStr | Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism |
| title_full_unstemmed | Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism |
| title_short | Embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation, as well as abnormalities in the TCA cycle and amino acid metabolism |
| title_sort | embryonic diapause due to high glucose is related to changes in glycolysis and oxidative phosphorylation as well as abnormalities in the tca cycle and amino acid metabolism |
| topic | embryo arrest high glucose embryo metabolism in vitro GLUTs GC-MS |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1135837/full |
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