Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications
Hydrogel with chemical modification has been used for 3D printing in the biomedical field of cell and tissue-based regeneration because it provides a good cellular microenvironment and mechanical supportive ability. As a scaffold and a matrix, hydrogel itself has to be modified chemically and physic...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/1/35 |
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| author | Heesun Hong Ok Joo Lee Young Jin Lee Ji Seung Lee Olatunji Ajiteru Hanna Lee Ye Ji Suh Md Tipu Sultan Soon Hee Kim Chan Hum Park |
| author_facet | Heesun Hong Ok Joo Lee Young Jin Lee Ji Seung Lee Olatunji Ajiteru Hanna Lee Ye Ji Suh Md Tipu Sultan Soon Hee Kim Chan Hum Park |
| author_sort | Heesun Hong |
| collection | DOAJ |
| description | Hydrogel with chemical modification has been used for 3D printing in the biomedical field of cell and tissue-based regeneration because it provides a good cellular microenvironment and mechanical supportive ability. As a scaffold and a matrix, hydrogel itself has to be modified chemically and physically to form a β-sheet crosslinking structure for the strength of the biomaterials. These chemical modifications could affect the biological damage done to encapsulated cells or surrounding tissues due to unreacted chemical residues. Biological assessment, including assessment of the cytocompatibility of hydrogel in clinical trials, must involve testing with cytotoxicity, irritation, and sensitization. Here, we modified silk fibroin and glycidyl methacrylate (Silk-GMA) and evaluated the physical characterizations, residual chemical detection, and the biological effect of residual GMA depending on dialysis periods. Silk-GMA depending on each dialysis period had a typical β-sheet structure in the characterization analysis and residual GMA decreased from dialysis day 1. Moreover, cell proliferation and viability rate gradually increased; additionally, necrotic and apoptotic cells decreased from dialysis day 2. These results indicate that the dialysis periods during chemical modification of natural polymer are important for removing unreacted chemical residues and for the potential application of the manufacturing standardization for chemically modified hydrogel for the clinical transplantation for tissue engineering and biomedical applications. |
| first_indexed | 2024-03-10T13:40:58Z |
| format | Article |
| id | doaj.art-b98a9121a73e475b9585b943369af920 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:40:58Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b98a9121a73e475b9585b943369af9202023-11-21T03:02:22ZengMDPI AGBiomolecules2218-273X2020-12-011113510.3390/biom11010035Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical ApplicationsHeesun Hong0Ok Joo Lee1Young Jin Lee2Ji Seung Lee3Olatunji Ajiteru4Hanna Lee5Ye Ji Suh6Md Tipu Sultan7Soon Hee Kim8Chan Hum Park9Nano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaNano-Bio Regenrative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, KoreaHydrogel with chemical modification has been used for 3D printing in the biomedical field of cell and tissue-based regeneration because it provides a good cellular microenvironment and mechanical supportive ability. As a scaffold and a matrix, hydrogel itself has to be modified chemically and physically to form a β-sheet crosslinking structure for the strength of the biomaterials. These chemical modifications could affect the biological damage done to encapsulated cells or surrounding tissues due to unreacted chemical residues. Biological assessment, including assessment of the cytocompatibility of hydrogel in clinical trials, must involve testing with cytotoxicity, irritation, and sensitization. Here, we modified silk fibroin and glycidyl methacrylate (Silk-GMA) and evaluated the physical characterizations, residual chemical detection, and the biological effect of residual GMA depending on dialysis periods. Silk-GMA depending on each dialysis period had a typical β-sheet structure in the characterization analysis and residual GMA decreased from dialysis day 1. Moreover, cell proliferation and viability rate gradually increased; additionally, necrotic and apoptotic cells decreased from dialysis day 2. These results indicate that the dialysis periods during chemical modification of natural polymer are important for removing unreacted chemical residues and for the potential application of the manufacturing standardization for chemically modified hydrogel for the clinical transplantation for tissue engineering and biomedical applications.https://www.mdpi.com/2218-273X/11/1/35silk fibroinchemical modificationglycidyl methacrylatedialysis periodscytocompatiblitytissue engineering |
| spellingShingle | Heesun Hong Ok Joo Lee Young Jin Lee Ji Seung Lee Olatunji Ajiteru Hanna Lee Ye Ji Suh Md Tipu Sultan Soon Hee Kim Chan Hum Park Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications Biomolecules silk fibroin chemical modification glycidyl methacrylate dialysis periods cytocompatiblity tissue engineering |
| title | Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications |
| title_full | Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications |
| title_fullStr | Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications |
| title_full_unstemmed | Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications |
| title_short | Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications |
| title_sort | cytocompatibility of modified silk fibroin with glycidyl methacrylate for tissue engineering and biomedical applications |
| topic | silk fibroin chemical modification glycidyl methacrylate dialysis periods cytocompatiblity tissue engineering |
| url | https://www.mdpi.com/2218-273X/11/1/35 |
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