Differentially expressed microRNAs as potential markers for vital reaction of burned skin
The identification of antemortem burns and postmortem burns is essential in forensic practice. In this study, microRNA (miRNA) microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model. Microarray analysis revealed 24 differentially expre...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Forensic Science and Medicine |
| Subjects: | |
| Online Access: | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=3;spage=135;epage=141;aulast=Lyu |
| _version_ | 1818498295647961088 |
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| author | Hao-Pin Lyu Ming Cheng Jin-Cen Liu Ming-Yuan Ye Di Xu Jie-Tao He Xiao-Li Xie Qi Wang |
| author_facet | Hao-Pin Lyu Ming Cheng Jin-Cen Liu Ming-Yuan Ye Di Xu Jie-Tao He Xiao-Li Xie Qi Wang |
| author_sort | Hao-Pin Lyu |
| collection | DOAJ |
| description | The identification of antemortem burns and postmortem burns is essential in forensic practice. In this study, microRNA (miRNA) microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model. Microarray analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin, with 19 miRNAs significantly upregulated and 5 downregulated. Based on the intersection predicted using three databases (Targetscan, microRNA.org, and PITA), 293 potential miRNA targets were identified. These dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Several functional categories and signaling pathways were enriched, including the “fc epsilon ri signaling pathway,” “endometrial cancer,” and “mTOR signaling pathway.” Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription-quantitative polymerase chain reaction in mice skins. The results agreed with the results of microarray analysis. These findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns. |
| first_indexed | 2024-12-10T18:56:21Z |
| format | Article |
| id | doaj.art-b98aca1a14844fc7a82899824cf6d7b0 |
| institution | Directory Open Access Journal |
| issn | 2349-5014 |
| language | English |
| last_indexed | 2024-12-10T18:56:21Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Forensic Science and Medicine |
| spelling | doaj.art-b98aca1a14844fc7a82899824cf6d7b02022-12-22T01:37:08ZengWolters Kluwer Medknow PublicationsJournal of Forensic Science and Medicine2349-50142018-01-014313514110.4103/jfsm.jfsm_1_18Differentially expressed microRNAs as potential markers for vital reaction of burned skinHao-Pin LyuMing ChengJin-Cen LiuMing-Yuan YeDi XuJie-Tao HeXiao-Li XieQi WangThe identification of antemortem burns and postmortem burns is essential in forensic practice. In this study, microRNA (miRNA) microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model. Microarray analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin, with 19 miRNAs significantly upregulated and 5 downregulated. Based on the intersection predicted using three databases (Targetscan, microRNA.org, and PITA), 293 potential miRNA targets were identified. These dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Several functional categories and signaling pathways were enriched, including the “fc epsilon ri signaling pathway,” “endometrial cancer,” and “mTOR signaling pathway.” Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription-quantitative polymerase chain reaction in mice skins. The results agreed with the results of microarray analysis. These findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns.http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=3;spage=135;epage=141;aulast=LyuForensic pathologymicroRNA microarrayskin burnsvital reaction |
| spellingShingle | Hao-Pin Lyu Ming Cheng Jin-Cen Liu Ming-Yuan Ye Di Xu Jie-Tao He Xiao-Li Xie Qi Wang Differentially expressed microRNAs as potential markers for vital reaction of burned skin Journal of Forensic Science and Medicine Forensic pathology microRNA microarray skin burns vital reaction |
| title | Differentially expressed microRNAs as potential markers for vital reaction of burned skin |
| title_full | Differentially expressed microRNAs as potential markers for vital reaction of burned skin |
| title_fullStr | Differentially expressed microRNAs as potential markers for vital reaction of burned skin |
| title_full_unstemmed | Differentially expressed microRNAs as potential markers for vital reaction of burned skin |
| title_short | Differentially expressed microRNAs as potential markers for vital reaction of burned skin |
| title_sort | differentially expressed micrornas as potential markers for vital reaction of burned skin |
| topic | Forensic pathology microRNA microarray skin burns vital reaction |
| url | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2018;volume=4;issue=3;spage=135;epage=141;aulast=Lyu |
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