A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency
One thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising or other bleeding manifestations, and excluding those with von Willebrand’s disease, were evaluated for platelet dense granule deficiency. The mean diameter of platelet dense granules...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/6/1734 |
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| author | William T. Gunning Meera Raghavan Edward P. Calomeni James N. Turner Bodri Roysam Shantala Roysam Mary R. Smith Peter A. Kouides Neil A. Lachant |
| author_facet | William T. Gunning Meera Raghavan Edward P. Calomeni James N. Turner Bodri Roysam Shantala Roysam Mary R. Smith Peter A. Kouides Neil A. Lachant |
| author_sort | William T. Gunning |
| collection | DOAJ |
| description | One thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising or other bleeding manifestations, and excluding those with von Willebrand’s disease, were evaluated for platelet dense granule deficiency. The mean diameter of platelet dense granules was determined for all patients using image analysis. Four hundred and ninety-nine had “classic” dense (delta) granule storage pool deficiency (δ-SPD). Five hundred and eighty-one individuals (53.8%) were found to have a normal mean number of dense granules, but for some of these patients, the dense granules were smaller than for the controls. Of the patients having a normal number of dense granules, 165 (28.4%) were found to have significantly smaller granules than the platelets obtained from the control subjects. Their average granule diameter was 123.35 ± 0.86 nm, that is more than three standard deviations below the mean of the control data. Total δ-granule storage pool volumes (TDGV)/platelet were calculated using these measurements. Individuals with δ-SPD had half the number of granules (2.25 ± 0.04 DG/PL) and storage pool volume (3.88 ± 1.06 × 10<sup>6</sup> nm<sup>3</sup>) when compared to our control data (4.64 ± 0.11 DG/PL; 10.79 × 10<sup>6</sup> nm<sup>3</sup> ± 0.42). Individuals having a bleeding history but a normal average of small dense granules had a calculated storage pool volume statistically different than controls and essentially the same storage pool volume as patients with δ-SPD. We have identified a sub-classification of δ-SPD that we have defined as micro-granular storage pool deficiency (δ-MGSPD). |
| first_indexed | 2024-03-10T19:23:06Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T19:23:06Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-b98bc028d26146d8ad284644627a26432023-11-20T02:48:33ZengMDPI AGJournal of Clinical Medicine2077-03832020-06-0196173410.3390/jcm9061734A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool DeficiencyWilliam T. Gunning0Meera Raghavan1Edward P. Calomeni2James N. Turner3Bodri Roysam4Shantala Roysam5Mary R. Smith6Peter A. Kouides7Neil A. Lachant8Department of Pathology, University of Ohio, Toledo, OH 43614, USAKansas City University of Medicine and Biosciences, Kansas City, MO 64106, USADepartment of Pathology, University of Ohio, Toledo, OH 43614, USANew York State Department of Health, Albany, NY 12203, USADepartment of Electrical and Computer Engineering, University of Houston, Houston, TX 77204, USAIndependent Researcher, Houston, TX 77002, USADepartment of Medicine, University of Toledo, Toledo, OH 43614, USAMary Gooley Hemophilia Center, Rochester General Hospital, Rochester, NY 14621, USAWilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY 14642, USAOne thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising or other bleeding manifestations, and excluding those with von Willebrand’s disease, were evaluated for platelet dense granule deficiency. The mean diameter of platelet dense granules was determined for all patients using image analysis. Four hundred and ninety-nine had “classic” dense (delta) granule storage pool deficiency (δ-SPD). Five hundred and eighty-one individuals (53.8%) were found to have a normal mean number of dense granules, but for some of these patients, the dense granules were smaller than for the controls. Of the patients having a normal number of dense granules, 165 (28.4%) were found to have significantly smaller granules than the platelets obtained from the control subjects. Their average granule diameter was 123.35 ± 0.86 nm, that is more than three standard deviations below the mean of the control data. Total δ-granule storage pool volumes (TDGV)/platelet were calculated using these measurements. Individuals with δ-SPD had half the number of granules (2.25 ± 0.04 DG/PL) and storage pool volume (3.88 ± 1.06 × 10<sup>6</sup> nm<sup>3</sup>) when compared to our control data (4.64 ± 0.11 DG/PL; 10.79 × 10<sup>6</sup> nm<sup>3</sup> ± 0.42). Individuals having a bleeding history but a normal average of small dense granules had a calculated storage pool volume statistically different than controls and essentially the same storage pool volume as patients with δ-SPD. We have identified a sub-classification of δ-SPD that we have defined as micro-granular storage pool deficiency (δ-MGSPD).https://www.mdpi.com/2077-0383/9/6/1734unexplained bleedingplatelet storage pool deficiencyplatelet dense granules |
| spellingShingle | William T. Gunning Meera Raghavan Edward P. Calomeni James N. Turner Bodri Roysam Shantala Roysam Mary R. Smith Peter A. Kouides Neil A. Lachant A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency Journal of Clinical Medicine unexplained bleeding platelet storage pool deficiency platelet dense granules |
| title | A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency |
| title_full | A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency |
| title_fullStr | A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency |
| title_full_unstemmed | A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency |
| title_short | A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency |
| title_sort | morphometric analysis of platelet dense granules of patients with unexplained bleeding a new entity of delta microgranular storage pool deficiency |
| topic | unexplained bleeding platelet storage pool deficiency platelet dense granules |
| url | https://www.mdpi.com/2077-0383/9/6/1734 |
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