Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i>
The isolation and chemical characterization of phlorotannins has gained special attention in recent years due to their specific health-promoting benefits. Flow-cell ultrasound-assisted extraction (90 W/cm<sup>2</sup> of sonication power, 2 min of retention time and 20 g solvent/g algae o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/11/706 |
| _version_ | 1797464784042459136 |
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| author | Mauro Gisbert Jorge Sineiro Ramón Moreira |
| author_facet | Mauro Gisbert Jorge Sineiro Ramón Moreira |
| author_sort | Mauro Gisbert |
| collection | DOAJ |
| description | The isolation and chemical characterization of phlorotannins has gained special attention in recent years due to their specific health-promoting benefits. Flow-cell ultrasound-assisted extraction (90 W/cm<sup>2</sup> of sonication power, 2 min of retention time and 20 g solvent/g algae of liquid–solid ratio) was carried out by using double-distilled water (WE) and acetone:water mixture (AWE) as extraction solvents. The AWE showed a higher total polyphenols content (TPC), carbohydrates (CHOs) and antioxidant activities than WE. However, when the WE was purified by using Amberlite XAD16 column, the purified WE (PWE) showed similar a TPC, decreased CHOs and increased antioxidant activity compared to WE. The oxidation of the PWE extract was evaluated under natural, forced and severe oxidation condition for 120 h. Only severe oxidation conditions were able to significantly reduce TPC and antioxidant activities. PWE was dialyzed (20, 10, 3.5 and 2 kDa). The main bioactive fraction of phlorotannins was obtained from 10 to 20 kDa. CHOs were distributed in fractions below 20 kDa. MALDI-TOF analysis was performed for PWE, PD20 and PD2 extracts to analyze the degree of polymerization of phlorotannins, which ranged from 4 to 17 phloroglucinol units/molecule. Fragmentation patterns allowed the proximate identification of several phlorotannins in <i>Ascophyllum nodosum</i> extracts. |
| first_indexed | 2024-03-09T18:13:05Z |
| format | Article |
| id | doaj.art-b993b138573a47e880a80c120a124e07 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T18:13:05Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-b993b138573a47e880a80c120a124e072023-11-24T08:59:53ZengMDPI AGMarine Drugs1660-33972022-11-01201170610.3390/md20110706Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i>Mauro Gisbert0Jorge Sineiro1Ramón Moreira2Chemical Engineering Department, Universidade de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, SpainChemical Engineering Department, Universidade de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, SpainChemical Engineering Department, Universidade de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, SpainThe isolation and chemical characterization of phlorotannins has gained special attention in recent years due to their specific health-promoting benefits. Flow-cell ultrasound-assisted extraction (90 W/cm<sup>2</sup> of sonication power, 2 min of retention time and 20 g solvent/g algae of liquid–solid ratio) was carried out by using double-distilled water (WE) and acetone:water mixture (AWE) as extraction solvents. The AWE showed a higher total polyphenols content (TPC), carbohydrates (CHOs) and antioxidant activities than WE. However, when the WE was purified by using Amberlite XAD16 column, the purified WE (PWE) showed similar a TPC, decreased CHOs and increased antioxidant activity compared to WE. The oxidation of the PWE extract was evaluated under natural, forced and severe oxidation condition for 120 h. Only severe oxidation conditions were able to significantly reduce TPC and antioxidant activities. PWE was dialyzed (20, 10, 3.5 and 2 kDa). The main bioactive fraction of phlorotannins was obtained from 10 to 20 kDa. CHOs were distributed in fractions below 20 kDa. MALDI-TOF analysis was performed for PWE, PD20 and PD2 extracts to analyze the degree of polymerization of phlorotannins, which ranged from 4 to 17 phloroglucinol units/molecule. Fragmentation patterns allowed the proximate identification of several phlorotannins in <i>Ascophyllum nodosum</i> extracts.https://www.mdpi.com/1660-3397/20/11/706phlorotanninsoxidationmolecular sizeDPPH radical scavenging activityFTIR<sup>1</sup>H-NMR |
| spellingShingle | Mauro Gisbert Jorge Sineiro Ramón Moreira Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> Marine Drugs phlorotannins oxidation molecular size DPPH radical scavenging activity FTIR <sup>1</sup>H-NMR |
| title | Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> |
| title_full | Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> |
| title_fullStr | Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> |
| title_full_unstemmed | Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> |
| title_short | Influence of Oxidation and Dialysis of Phlorotannins on Bioactivity and Composition of Ultrasound-Assisted Extracts from <i>Ascophyllum nodosum</i> |
| title_sort | influence of oxidation and dialysis of phlorotannins on bioactivity and composition of ultrasound assisted extracts from i ascophyllum nodosum i |
| topic | phlorotannins oxidation molecular size DPPH radical scavenging activity FTIR <sup>1</sup>H-NMR |
| url | https://www.mdpi.com/1660-3397/20/11/706 |
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