Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products
Botanical biopesticides have emerged as sustainable and eco-friendly alternatives to synthetic pesticides, whose indiscriminate use leads to several drawbacks to human and environmental health. To the best of our knowledge, there have been no reports on <i>M. pubescens</i>’ bioactivity o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/11/6736 |
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| author | Acácio R. A. Moraes Samuel R. Sabina Daniela G. Expósito Cristina Giménez Guacimara Espinel Grasiely F. Sousa Lucienir P. Duarte Ignacio A. Jiménez Raimundo Cabrera Isabel L. Bazzocchi |
| author_facet | Acácio R. A. Moraes Samuel R. Sabina Daniela G. Expósito Cristina Giménez Guacimara Espinel Grasiely F. Sousa Lucienir P. Duarte Ignacio A. Jiménez Raimundo Cabrera Isabel L. Bazzocchi |
| author_sort | Acácio R. A. Moraes |
| collection | DOAJ |
| description | Botanical biopesticides have emerged as sustainable and eco-friendly alternatives to synthetic pesticides, whose indiscriminate use leads to several drawbacks to human and environmental health. To the best of our knowledge, there have been no reports on <i>M. pubescens</i>’ bioactivity on phytopathogens affecting crops as a potential fungicide or antifeedant. This has encouraged us to investigate the potential of the roots of this plant as a source of biopesticides. The present study reports on the evaluation of the roots extract from <i>Magonia pubescens</i> A. St.-Hill., a species from the Cerrado (Brazilian savannah), on the phytopathogenic fungi <i>Botrytis cinerea</i>, <i>Fusarium oxysporum</i>, and <i>Alternaria alternata</i>. In addition, its insect antifeedant effect was assayed against <i>Chrysodeixis chalcites</i>. Thus, an in vitro test-assay was used to determine the fungicide potential (percentage growth inhibition, % GI) of the ethanolic extract of this plant species, whereas a leaf-disk bioassay on the 5th instar larvae of <i>C. chalcites</i> was performed to evaluate its insecticidal potential. The ethanolic extract was further fractionated by liquid–liquid partition using solvents of increasing polarity. The hexane/dichloromethane fraction exhibited a moderated potency and was similar to the ethanolic extract on the three assayed fungi (around % GI 30 at 1 mg/mL), whereas the <i>n</i>-butanol fraction showed a slight improvement of the fungicide effect against <i>B. cinerea</i> (% GI 39.18 at 1 mg/mL). Moreover, the ethanolic extract exhibited a strong antifeedant activity, with a refusal rate (FR) higher than 90% in both choice and non-choice assays against <i>C. chalcites</i>, while the ethyl acetate and <i>n</i>-butanol fractions behaved as appetite suppressors. These results highlight <i>M. pubescens</i> as a promising source of biopesticides and deserve further investigations to optimize extraction procedures. |
| first_indexed | 2024-03-11T03:11:46Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T03:11:46Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-b995bc433b454071b6a2614ae8ef40aa2023-11-18T07:36:04ZengMDPI AGApplied Sciences2076-34172023-06-011311673610.3390/app13116736Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection ProductsAcácio R. A. Moraes0Samuel R. Sabina1Daniela G. Expósito2Cristina Giménez3Guacimara Espinel4Grasiely F. Sousa5Lucienir P. Duarte6Ignacio A. Jiménez7Raimundo Cabrera8Isabel L. Bazzocchi9Instituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainDepartamento de Botánica, Ecología y Fisiología Vegetal, Facultad de Biología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainInstituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainDepartamento de Botánica, Ecología y Fisiología Vegetal, Facultad de Biología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainDepartamento de Botánica, Ecología y Fisiología Vegetal, Facultad de Biología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, Belo Horizonte 31270-901, MG, BrazilInstituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainDepartamento de Botánica, Ecología y Fisiología Vegetal, Facultad de Biología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainInstituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, SpainBotanical biopesticides have emerged as sustainable and eco-friendly alternatives to synthetic pesticides, whose indiscriminate use leads to several drawbacks to human and environmental health. To the best of our knowledge, there have been no reports on <i>M. pubescens</i>’ bioactivity on phytopathogens affecting crops as a potential fungicide or antifeedant. This has encouraged us to investigate the potential of the roots of this plant as a source of biopesticides. The present study reports on the evaluation of the roots extract from <i>Magonia pubescens</i> A. St.-Hill., a species from the Cerrado (Brazilian savannah), on the phytopathogenic fungi <i>Botrytis cinerea</i>, <i>Fusarium oxysporum</i>, and <i>Alternaria alternata</i>. In addition, its insect antifeedant effect was assayed against <i>Chrysodeixis chalcites</i>. Thus, an in vitro test-assay was used to determine the fungicide potential (percentage growth inhibition, % GI) of the ethanolic extract of this plant species, whereas a leaf-disk bioassay on the 5th instar larvae of <i>C. chalcites</i> was performed to evaluate its insecticidal potential. The ethanolic extract was further fractionated by liquid–liquid partition using solvents of increasing polarity. The hexane/dichloromethane fraction exhibited a moderated potency and was similar to the ethanolic extract on the three assayed fungi (around % GI 30 at 1 mg/mL), whereas the <i>n</i>-butanol fraction showed a slight improvement of the fungicide effect against <i>B. cinerea</i> (% GI 39.18 at 1 mg/mL). Moreover, the ethanolic extract exhibited a strong antifeedant activity, with a refusal rate (FR) higher than 90% in both choice and non-choice assays against <i>C. chalcites</i>, while the ethyl acetate and <i>n</i>-butanol fractions behaved as appetite suppressors. These results highlight <i>M. pubescens</i> as a promising source of biopesticides and deserve further investigations to optimize extraction procedures.https://www.mdpi.com/2076-3417/13/11/6736<i>Magonia pubescens</i>biopesticidesphytopathogenic fungi<i>Chrysodeixis chalcites</i> |
| spellingShingle | Acácio R. A. Moraes Samuel R. Sabina Daniela G. Expósito Cristina Giménez Guacimara Espinel Grasiely F. Sousa Lucienir P. Duarte Ignacio A. Jiménez Raimundo Cabrera Isabel L. Bazzocchi Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products Applied Sciences <i>Magonia pubescens</i> biopesticides phytopathogenic fungi <i>Chrysodeixis chalcites</i> |
| title | Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products |
| title_full | Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products |
| title_fullStr | Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products |
| title_full_unstemmed | Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products |
| title_short | Evaluation of <i>Magonia pubescens</i> A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products |
| title_sort | evaluation of i magonia pubescens i a st hill roots extract against phytopathogens searching for eco friendly crop protection products |
| topic | <i>Magonia pubescens</i> biopesticides phytopathogenic fungi <i>Chrysodeixis chalcites</i> |
| url | https://www.mdpi.com/2076-3417/13/11/6736 |
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