AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES
The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential...
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal Rural de Pernambuco
2023-12-01
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| Series: | Medicina Veterinária |
| Subjects: | |
| Online Access: | https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897 |
| _version_ | 1797373090051653632 |
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| author | Maria Fernanda Cazini Silva Luma Maria Rovina Brito Beatriz de Azevedo Martins Laryssa Eduarda de Campos Lopes Yasmin Cunha Duarte Matheus Nogueira Morais Laura Arduino Vasconcelos Mariana Orlandini Mendonça Tainara de Oliveira Martins Daniela Fernandez Montechiesi Breno Fernando Martins de Almeida |
| author_facet | Maria Fernanda Cazini Silva Luma Maria Rovina Brito Beatriz de Azevedo Martins Laryssa Eduarda de Campos Lopes Yasmin Cunha Duarte Matheus Nogueira Morais Laura Arduino Vasconcelos Mariana Orlandini Mendonça Tainara de Oliveira Martins Daniela Fernandez Montechiesi Breno Fernando Martins de Almeida |
| author_sort | Maria Fernanda Cazini Silva |
| collection | DOAJ |
| description |
The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.
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| first_indexed | 2024-03-08T18:45:19Z |
| format | Article |
| id | doaj.art-b996e1bc1420451fad5be48329180c73 |
| institution | Directory Open Access Journal |
| issn | 1809-4678 2675-6617 |
| language | English |
| last_indexed | 2024-03-08T18:45:19Z |
| publishDate | 2023-12-01 |
| publisher | Universidade Federal Rural de Pernambuco |
| record_format | Article |
| series | Medicina Veterinária |
| spelling | doaj.art-b996e1bc1420451fad5be48329180c732023-12-29T00:29:27ZengUniversidade Federal Rural de PernambucoMedicina Veterinária1809-46782675-66172023-12-0117410.26605/medvet-v17n4-5897AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINESMaria Fernanda Cazini Silva 0Luma Maria Rovina Brito1Beatriz de Azevedo Martins2Laryssa Eduarda de Campos Lopes3Yasmin Cunha Duarte4Matheus Nogueira Morais5Laura Arduino Vasconcelos6Mariana Orlandini Mendonça7Tainara de Oliveira Martins8Daniela Fernandez Montechiesi9Breno Fernando Martins de Almeida10Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, BrazilOurinhos Veterinary Hospital (HVO), Ourinhos-SP, BrazilCanguru Veterinary Hospital, Ourinhos-SP, BrazilAutonomous Veterinary, Ourinhos-SP, BrazilDepartment of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil. Faculty of Veterinary Medicine of Araçatuba, São Paulo State University, (FMVA-Unesp), Araçatuba-SP, Brazil The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses. https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897HorseLeukogramImpedance |
| spellingShingle | Maria Fernanda Cazini Silva Luma Maria Rovina Brito Beatriz de Azevedo Martins Laryssa Eduarda de Campos Lopes Yasmin Cunha Duarte Matheus Nogueira Morais Laura Arduino Vasconcelos Mariana Orlandini Mendonça Tainara de Oliveira Martins Daniela Fernandez Montechiesi Breno Fernando Martins de Almeida AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES Medicina Veterinária Horse Leukogram Impedance |
| title | AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES |
| title_full | AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES |
| title_fullStr | AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES |
| title_full_unstemmed | AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES |
| title_short | AUTOMATED AND MANUAL LEUKOCYTE DIFFERENTIAL COUNTS IN HEALTHY EQUINES |
| title_sort | automated and manual leukocyte differential counts in healthy equines |
| topic | Horse Leukogram Impedance |
| url | https://www.journals.ufrpe.br/index.php/medicinaveterinaria/article/view/5897 |
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