First approach to molecular epidemiology of bovine tuberculosis in Colombia
Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of...
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| Format: | Article |
| Language: | English |
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Universidad de Cordoba
2015-12-01
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| Series: | Revista MVZ Cordoba |
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| Online Access: | http://revistas.unicordoba.edu.co/revistamvz/mvz-211/pdf/v21n1a13.pdf |
| _version_ | 1811212644887035904 |
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| author | Jimena Jojoa-Jojoa Maira Wintaco M. Francisco Osorio R. Gloria Puerto-Castro Martha Guerrero-Guerrero |
| author_facet | Jimena Jojoa-Jojoa Maira Wintaco M. Francisco Osorio R. Gloria Puerto-Castro Martha Guerrero-Guerrero |
| author_sort | Jimena Jojoa-Jojoa |
| collection | DOAJ |
| description | Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health. |
| first_indexed | 2024-04-12T05:33:37Z |
| format | Article |
| id | doaj.art-b9970435fadb4553b9219b213d1ea99d |
| institution | Directory Open Access Journal |
| issn | 0122-0268 1909-0544 |
| language | English |
| last_indexed | 2024-04-12T05:33:37Z |
| publishDate | 2015-12-01 |
| publisher | Universidad de Cordoba |
| record_format | Article |
| series | Revista MVZ Cordoba |
| spelling | doaj.art-b9970435fadb4553b9219b213d1ea99d2022-12-22T03:45:59ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442015-12-0121152225236First approach to molecular epidemiology of bovine tuberculosis in ColombiaJimena Jojoa-Jojoa0Maira Wintaco M.1Francisco Osorio R.2Gloria Puerto-Castro3Martha Guerrero-Guerrero4Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.Instituto Colombiano Agropecuario ICA, Programa de Brucelosis y Tuberculosis bovina, Carrera 41 #17-81 Bogotá D.C. Colombia.Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.Instituto Nacional de Salud, Dirección de Investigación en Salud Pública, Grupo de Micobacterias, Avenida Calle 26 # 51-20 Bogotá D.C. Colombia.Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.http://revistas.unicordoba.edu.co/revistamvz/mvz-211/pdf/v21n1a13.pdfBovine tuberculosisMycobacterium bovisdiagnosticmolecular epidemiologyzoonosis |
| spellingShingle | Jimena Jojoa-Jojoa Maira Wintaco M. Francisco Osorio R. Gloria Puerto-Castro Martha Guerrero-Guerrero First approach to molecular epidemiology of bovine tuberculosis in Colombia Revista MVZ Cordoba Bovine tuberculosis Mycobacterium bovis diagnostic molecular epidemiology zoonosis |
| title | First approach to molecular epidemiology of bovine tuberculosis in Colombia |
| title_full | First approach to molecular epidemiology of bovine tuberculosis in Colombia |
| title_fullStr | First approach to molecular epidemiology of bovine tuberculosis in Colombia |
| title_full_unstemmed | First approach to molecular epidemiology of bovine tuberculosis in Colombia |
| title_short | First approach to molecular epidemiology of bovine tuberculosis in Colombia |
| title_sort | first approach to molecular epidemiology of bovine tuberculosis in colombia |
| topic | Bovine tuberculosis Mycobacterium bovis diagnostic molecular epidemiology zoonosis |
| url | http://revistas.unicordoba.edu.co/revistamvz/mvz-211/pdf/v21n1a13.pdf |
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