Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries
Abstract Background In low- and middle-income countries (LMIC) Staphylococcus aureus is regarded as one of the leading bacterial causes of neonatal sepsis, however there is limited knowledge on the species diversity and antimicrobial resistance caused by Gram-positive bacteria (GPB). Methods We char...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-07-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-022-07541-w |
| _version_ | 1811219000224382976 |
|---|---|
| author | Kirsty Sands Maria J. Carvalho Owen B. Spiller Edward A. R. Portal Kathryn Thomson William John Watkins Jordan Mathias Calie Dyer Chinenye Akpulu Robert Andrews Ana Ferreira Thomas Hender Rebecca Milton Maria Nieto Rabaab Zahra Haider Shirazi Adil Muhammad Shermeen Akif Muhammad Hilal Jan Kenneth Iregbu Fatima Modibbo Stella Uwaezuoke Grace J. Chan Delayehu Bekele Semaria Solomon Sulagna Basu Ranjan Kumar Nandy Sharmi Naha Jean-Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andre N. H. Bulabula Andrew Whitelaw BARNARDS Group Timothy R. Walsh |
| author_facet | Kirsty Sands Maria J. Carvalho Owen B. Spiller Edward A. R. Portal Kathryn Thomson William John Watkins Jordan Mathias Calie Dyer Chinenye Akpulu Robert Andrews Ana Ferreira Thomas Hender Rebecca Milton Maria Nieto Rabaab Zahra Haider Shirazi Adil Muhammad Shermeen Akif Muhammad Hilal Jan Kenneth Iregbu Fatima Modibbo Stella Uwaezuoke Grace J. Chan Delayehu Bekele Semaria Solomon Sulagna Basu Ranjan Kumar Nandy Sharmi Naha Jean-Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andre N. H. Bulabula Andrew Whitelaw BARNARDS Group Timothy R. Walsh |
| author_sort | Kirsty Sands |
| collection | DOAJ |
| description | Abstract Background In low- and middle-income countries (LMIC) Staphylococcus aureus is regarded as one of the leading bacterial causes of neonatal sepsis, however there is limited knowledge on the species diversity and antimicrobial resistance caused by Gram-positive bacteria (GPB). Methods We characterised GPB isolates from neonatal blood cultures from LMICs in Africa (Ethiopia, Nigeria, Rwanda, and South Africa) and South-Asia (Bangladesh and Pakistan) between 2015–2017. We determined minimum inhibitory concentrations and performed whole genome sequencing (WGS) on Staphylococci isolates recovered and clinical data collected related to the onset of sepsis and the outcome of the neonate up to 60 days of age. Results From the isolates recovered from blood cultures, Staphylococci species were most frequently identified. Out of 100 S. aureus isolates sequenced, 18 different sequence types (ST) were found which unveiled two small epidemiological clusters caused by methicillin resistant S. aureus (MRSA) in Pakistan (ST8) and South Africa (ST5), both with high mortality (n = 6/17). One-third of S. aureus was MRSA, with methicillin resistance also detected in Staphylococcus epidermidis, Staphylococcus haemolyticus and Mammaliicoccus sciuri. Through additional WGS analysis we report a cluster of M. sciuri in Pakistan identified between July-November 2017. Conclusions In total we identified 14 different GPB bacterial species, however Staphylococci was dominant. These findings highlight the need of a prospective genomic epidemiology study to comprehensively assess the true burden of GPB neonatal sepsis focusing specifically on mechanisms of resistance and virulence across species and in relation to neonatal outcome. |
| first_indexed | 2024-04-12T07:18:35Z |
| format | Article |
| id | doaj.art-b99a40e2deec4c4c8a38edd2aca6709a |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-12T07:18:35Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-b99a40e2deec4c4c8a38edd2aca6709a2022-12-22T03:42:23ZengBMCBMC Infectious Diseases1471-23342022-07-0122111610.1186/s12879-022-07541-wCharacterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countriesKirsty Sands0Maria J. Carvalho1Owen B. Spiller2Edward A. R. Portal3Kathryn Thomson4William John Watkins5Jordan Mathias6Calie Dyer7Chinenye Akpulu8Robert Andrews9Ana Ferreira10Thomas Hender11Rebecca Milton12Maria Nieto13Rabaab Zahra14Haider Shirazi15Adil Muhammad16Shermeen Akif17Muhammad Hilal Jan18Kenneth Iregbu19Fatima Modibbo20Stella Uwaezuoke21Grace J. Chan22Delayehu Bekele23Semaria Solomon24Sulagna Basu25Ranjan Kumar Nandy26Sharmi Naha27Jean-Baptiste Mazarati28Aniceth Rucogoza29Lucie Gaju30Shaheen Mehtar31Andre N. H. Bulabula32Andrew Whitelaw33BARNARDS GroupTimothy R. Walsh34Department of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityDepartment of Microbiology, Quaid-I-Azam UniversityPakistan Institute of Medical SciencesDepartment of Microbiology, Quaid-I-Azam UniversityDepartment of Microbiology, Quaid-I-Azam UniversityDepartment of Microbiology, Quaid-I-Azam UniversityNational Hospital AbujaMurtala Muhammad Specialist HospitalFederal Medical Centre JabiDivision of Medical Critical Care, Boston Children’s HospitalDepartment of Obstetrics and Gynecology, St Paul’s Hospital Millennium Medical CollegeDepartment of Microbiology, Immunology and Parasitology, St Paul’s Hospital Millennium Medical CollegeDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases BeliaghataDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases BeliaghataDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases BeliaghataRwanda Biomedical Centre, The National Reference LaboratoryRwanda Biomedical Centre, The National Reference LaboratoryRwanda Biomedical Centre, The National Reference LaboratoryUnit of IPC, Stellenbosch UniversityInfection Control Africa NetworkDivision of Medical Microbiology, Stellenbosch UniversityDepartment of Medical Microbiology, Division of Infection and Immunity, School of Medicine, Cardiff UniversityAbstract Background In low- and middle-income countries (LMIC) Staphylococcus aureus is regarded as one of the leading bacterial causes of neonatal sepsis, however there is limited knowledge on the species diversity and antimicrobial resistance caused by Gram-positive bacteria (GPB). Methods We characterised GPB isolates from neonatal blood cultures from LMICs in Africa (Ethiopia, Nigeria, Rwanda, and South Africa) and South-Asia (Bangladesh and Pakistan) between 2015–2017. We determined minimum inhibitory concentrations and performed whole genome sequencing (WGS) on Staphylococci isolates recovered and clinical data collected related to the onset of sepsis and the outcome of the neonate up to 60 days of age. Results From the isolates recovered from blood cultures, Staphylococci species were most frequently identified. Out of 100 S. aureus isolates sequenced, 18 different sequence types (ST) were found which unveiled two small epidemiological clusters caused by methicillin resistant S. aureus (MRSA) in Pakistan (ST8) and South Africa (ST5), both with high mortality (n = 6/17). One-third of S. aureus was MRSA, with methicillin resistance also detected in Staphylococcus epidermidis, Staphylococcus haemolyticus and Mammaliicoccus sciuri. Through additional WGS analysis we report a cluster of M. sciuri in Pakistan identified between July-November 2017. Conclusions In total we identified 14 different GPB bacterial species, however Staphylococci was dominant. These findings highlight the need of a prospective genomic epidemiology study to comprehensively assess the true burden of GPB neonatal sepsis focusing specifically on mechanisms of resistance and virulence across species and in relation to neonatal outcome.https://doi.org/10.1186/s12879-022-07541-wLMICStaphylococciNeonatal sepsisGenomicsMammaliicocciMortality |
| spellingShingle | Kirsty Sands Maria J. Carvalho Owen B. Spiller Edward A. R. Portal Kathryn Thomson William John Watkins Jordan Mathias Calie Dyer Chinenye Akpulu Robert Andrews Ana Ferreira Thomas Hender Rebecca Milton Maria Nieto Rabaab Zahra Haider Shirazi Adil Muhammad Shermeen Akif Muhammad Hilal Jan Kenneth Iregbu Fatima Modibbo Stella Uwaezuoke Grace J. Chan Delayehu Bekele Semaria Solomon Sulagna Basu Ranjan Kumar Nandy Sharmi Naha Jean-Baptiste Mazarati Aniceth Rucogoza Lucie Gaju Shaheen Mehtar Andre N. H. Bulabula Andrew Whitelaw BARNARDS Group Timothy R. Walsh Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries BMC Infectious Diseases LMIC Staphylococci Neonatal sepsis Genomics Mammaliicocci Mortality |
| title | Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries |
| title_full | Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries |
| title_fullStr | Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries |
| title_full_unstemmed | Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries |
| title_short | Characterisation of Staphylococci species from neonatal blood cultures in low- and middle-income countries |
| title_sort | characterisation of staphylococci species from neonatal blood cultures in low and middle income countries |
| topic | LMIC Staphylococci Neonatal sepsis Genomics Mammaliicocci Mortality |
| url | https://doi.org/10.1186/s12879-022-07541-w |
| work_keys_str_mv | AT kirstysands characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT mariajcarvalho characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT owenbspiller characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT edwardarportal characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT kathrynthomson characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT williamjohnwatkins characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT jordanmathias characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT caliedyer characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT chinenyeakpulu characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT robertandrews characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT anaferreira characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT thomashender characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT rebeccamilton characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT marianieto characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT rabaabzahra characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT haidershirazi characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT adilmuhammad characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT shermeenakif characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT muhammadhilaljan characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT kennethiregbu characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT fatimamodibbo characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT stellauwaezuoke characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT gracejchan characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT delayehubekele characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT semariasolomon characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT sulagnabasu characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT ranjankumarnandy characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT sharminaha characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT jeanbaptistemazarati characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT anicethrucogoza characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT luciegaju characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT shaheenmehtar characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT andrenhbulabula characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT andrewwhitelaw characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT barnardsgroup characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries AT timothyrwalsh characterisationofstaphylococcispeciesfromneonatalbloodculturesinlowandmiddleincomecountries |