Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects
The rhizosphere is a rich source of actinomycetes which can produce several potential biologically active secondary metabolites. The principal goal for this research is to extract, purify, and characterize the bioactive secondary metabolites produced by three different strains of actinomycetes isola...
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/9/1869 |
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| author | Hazem S. Elshafie Laura De Martino Carmen Formisano Lucia Caputo Vincenzo De Feo Ippolito Camele |
| author_facet | Hazem S. Elshafie Laura De Martino Carmen Formisano Lucia Caputo Vincenzo De Feo Ippolito Camele |
| author_sort | Hazem S. Elshafie |
| collection | DOAJ |
| description | The rhizosphere is a rich source of actinomycetes which can produce several potential biologically active secondary metabolites. The principal goal for this research is to extract, purify, and characterize the bioactive secondary metabolites produced by three different strains of actinomycetes isolated from the rhizosphere of rosemary, black locust, and olive. The plant growth-promoting effect (PGPE) of the studied strains of actinomycetes on <i>Ocimum basilicum</i> L. (basil) and the disease-control effect on necrotic stem lesions of “black leg” caused by <i>Fusarium tabacinum</i> on basil were evaluated in silico. The cell-free culture filtrates from the studied actinomycetes isolates were evaluated in vitro for their antimicrobial activity against some common phytopathogens. The secondary metabolites obtained from the cell-free culture filtrates have been chemically characterized using high-resolution electrospray ionization of liquid-chromatography/mass-spectrometric detection (ESI-(HR)Orbitrap-MS). Results of the in silico trial showed that all studied isolates demonstrated PGPE on basil seedlings, improved some eco-physiological characteristics, and reduced the disease incidence of <i>F. tabacinum</i>. The extracted metabolites from the studied actinomycetes demonstrated antimicrobial activity in a Petri-plates assay. The chemical analysis revealed the presence of 20 different components. This research emphasizes how valuable the examined isolates are for producing bioactive compounds, indicating their putative antimicrobial activity and their potential employment as fungal biocontrol agents. In particular, the obtained results revealed the possibility of green synthesis of some important secondary metabolites, such as <i>N</i>-Acetyl-<span style="font-variant: small-caps;">l</span>-histidinol, Rhizocticin A, and Eponemycin, from actinomycetes. The bioactive metabolites may be successively used to develop novel bio-formulations for both crop protection and/or PGPE. |
| first_indexed | 2024-03-11T04:10:22Z |
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| language | English |
| last_indexed | 2024-03-11T04:10:22Z |
| publishDate | 2023-05-01 |
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| series | Plants |
| spelling | doaj.art-b99b5d55440e4ea09b619424b972eefb2023-11-17T23:32:49ZengMDPI AGPlants2223-77472023-05-01129186910.3390/plants12091869Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting EffectsHazem S. Elshafie0Laura De Martino1Carmen Formisano2Lucia Caputo3Vincenzo De Feo4Ippolito Camele5School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, 85100 Potenza, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Montesano 49, 80131 Naples, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, ItalySchool of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, 85100 Potenza, ItalyThe rhizosphere is a rich source of actinomycetes which can produce several potential biologically active secondary metabolites. The principal goal for this research is to extract, purify, and characterize the bioactive secondary metabolites produced by three different strains of actinomycetes isolated from the rhizosphere of rosemary, black locust, and olive. The plant growth-promoting effect (PGPE) of the studied strains of actinomycetes on <i>Ocimum basilicum</i> L. (basil) and the disease-control effect on necrotic stem lesions of “black leg” caused by <i>Fusarium tabacinum</i> on basil were evaluated in silico. The cell-free culture filtrates from the studied actinomycetes isolates were evaluated in vitro for their antimicrobial activity against some common phytopathogens. The secondary metabolites obtained from the cell-free culture filtrates have been chemically characterized using high-resolution electrospray ionization of liquid-chromatography/mass-spectrometric detection (ESI-(HR)Orbitrap-MS). Results of the in silico trial showed that all studied isolates demonstrated PGPE on basil seedlings, improved some eco-physiological characteristics, and reduced the disease incidence of <i>F. tabacinum</i>. The extracted metabolites from the studied actinomycetes demonstrated antimicrobial activity in a Petri-plates assay. The chemical analysis revealed the presence of 20 different components. This research emphasizes how valuable the examined isolates are for producing bioactive compounds, indicating their putative antimicrobial activity and their potential employment as fungal biocontrol agents. In particular, the obtained results revealed the possibility of green synthesis of some important secondary metabolites, such as <i>N</i>-Acetyl-<span style="font-variant: small-caps;">l</span>-histidinol, Rhizocticin A, and Eponemycin, from actinomycetes. The bioactive metabolites may be successively used to develop novel bio-formulations for both crop protection and/or PGPE.https://www.mdpi.com/2223-7747/12/9/1869antimicrobial activitynatural productsmicrobial metabolitesplant diseasesbiological control |
| spellingShingle | Hazem S. Elshafie Laura De Martino Carmen Formisano Lucia Caputo Vincenzo De Feo Ippolito Camele Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects Plants antimicrobial activity natural products microbial metabolites plant diseases biological control |
| title | Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects |
| title_full | Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects |
| title_fullStr | Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects |
| title_full_unstemmed | Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects |
| title_short | Chemical Identification of Secondary Metabolites from Rhizospheric Actinomycetes Using LC-MS Analysis: In Silico Antifungal Evaluation and Growth-Promoting Effects |
| title_sort | chemical identification of secondary metabolites from rhizospheric actinomycetes using lc ms analysis in silico antifungal evaluation and growth promoting effects |
| topic | antimicrobial activity natural products microbial metabolites plant diseases biological control |
| url | https://www.mdpi.com/2223-7747/12/9/1869 |
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