Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>)
Indirect defenses are plant phenotypes that reduce damage by attracting natural enemies of plant pests and pathogens to leaves. Despite their economic and ecological importance, few studies have investigated the genetic underpinnings of indirect defense phenotypes. Here, we present a genome-wide ass...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/7/1013 |
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| author | Erika R. LaPlante Margaret B. Fleming Zoë Migicovsky Marjorie Gail Weber |
| author_facet | Erika R. LaPlante Margaret B. Fleming Zoë Migicovsky Marjorie Gail Weber |
| author_sort | Erika R. LaPlante |
| collection | DOAJ |
| description | Indirect defenses are plant phenotypes that reduce damage by attracting natural enemies of plant pests and pathogens to leaves. Despite their economic and ecological importance, few studies have investigated the genetic underpinnings of indirect defense phenotypes. Here, we present a genome-wide association study of five phenotypes previously determined to increase populations of beneficial (fungivorous and predacious) mites on grape leaves (genus <i>Vitis</i>): leaf bristles, leaf hairs, and the size, density, and depth of leaf domatia. Using a common garden genetic panel of 399 <i>V. vinifera</i> cultivars, we tested for genetic associations of these phenotypes using previously obtained genotyping data from the Vitis9kSNP array. We found one single nucleotide polymorphism (SNP) significantly associated with domatia density. This SNP (Chr5:1160194) is near two genes of interest: <i>Importin Alpha Isoform 1</i> (VIT_205s0077g01440), involved in downy mildew resistance, and <i>GATA Transcription Factor 8</i> (VIT_205s0077g01450), involved in leaf shape development. Our findings are among the first to examine the genomic regions associated with ecologically important plant traits that facilitate interactions with beneficial mites, and suggest promising candidate genes for breeding and genetic editing to increase naturally occurring predator-based defenses in grapevines. |
| first_indexed | 2024-03-10T09:56:04Z |
| format | Article |
| id | doaj.art-b99ebdc7739c4fd18f8946bdcfcfc525 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T09:56:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-b99ebdc7739c4fd18f8946bdcfcfc5252023-11-22T02:20:39ZengMDPI AGGenes2073-44252021-06-01127101310.3390/genes12071013Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>)Erika R. LaPlante0Margaret B. Fleming1Zoë Migicovsky2Marjorie Gail Weber3Department of Plant Biology, Program in Ecology, Evolution, and Behavior, Michigan State University, East Lansing, MI 48824, USADepartment of Plant Biology, Program in Ecology, Evolution, and Behavior, Michigan State University, East Lansing, MI 48824, USADepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, CanadaDepartment of Plant Biology, Program in Ecology, Evolution, and Behavior, Michigan State University, East Lansing, MI 48824, USAIndirect defenses are plant phenotypes that reduce damage by attracting natural enemies of plant pests and pathogens to leaves. Despite their economic and ecological importance, few studies have investigated the genetic underpinnings of indirect defense phenotypes. Here, we present a genome-wide association study of five phenotypes previously determined to increase populations of beneficial (fungivorous and predacious) mites on grape leaves (genus <i>Vitis</i>): leaf bristles, leaf hairs, and the size, density, and depth of leaf domatia. Using a common garden genetic panel of 399 <i>V. vinifera</i> cultivars, we tested for genetic associations of these phenotypes using previously obtained genotyping data from the Vitis9kSNP array. We found one single nucleotide polymorphism (SNP) significantly associated with domatia density. This SNP (Chr5:1160194) is near two genes of interest: <i>Importin Alpha Isoform 1</i> (VIT_205s0077g01440), involved in downy mildew resistance, and <i>GATA Transcription Factor 8</i> (VIT_205s0077g01450), involved in leaf shape development. Our findings are among the first to examine the genomic regions associated with ecologically important plant traits that facilitate interactions with beneficial mites, and suggest promising candidate genes for breeding and genetic editing to increase naturally occurring predator-based defenses in grapevines.https://www.mdpi.com/2073-4425/12/7/1013acarodomatiadefense mutualismsindirect defensemite domatiamite defensepowdery mildew |
| spellingShingle | Erika R. LaPlante Margaret B. Fleming Zoë Migicovsky Marjorie Gail Weber Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) Genes acarodomatia defense mutualisms indirect defense mite domatia mite defense powdery mildew |
| title | Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) |
| title_full | Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) |
| title_fullStr | Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) |
| title_full_unstemmed | Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) |
| title_short | Genome-Wide Association Study Reveals a Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine (<i>Vitis vinifera</i>) |
| title_sort | genome wide association study reveals a genomic region associated with mite recruitment phenotypes in the domesticated grapevine i vitis vinifera i |
| topic | acarodomatia defense mutualisms indirect defense mite domatia mite defense powdery mildew |
| url | https://www.mdpi.com/2073-4425/12/7/1013 |
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