Engipore acts on human bone marrow stem cells
Objectives: Porous HA scaffolds are promising materials for tissue engineering because they offer a tridimensional support and serve as template for cell proliferation and at last tissue formation. Engipore provide a natural 3D scaffold with organic fibrous material in bone. However, how this materi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-10-01
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| Series: | Saudi Dental Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905210000696 |
| _version_ | 1818872663675764736 |
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| author | Vincenzo Sollazzo Annalisa Palmieri Ambra Girardi Francesca Farinella Francesco Carinci |
| author_facet | Vincenzo Sollazzo Annalisa Palmieri Ambra Girardi Francesca Farinella Francesco Carinci |
| author_sort | Vincenzo Sollazzo |
| collection | DOAJ |
| description | Objectives: Porous HA scaffolds are promising materials for tissue engineering because they offer a tridimensional support and serve as template for cell proliferation and at last tissue formation. Engipore provide a natural 3D scaffold with organic fibrous material in bone. However, how this material alters osteoblast activity to promote bone formation is poorly understood. Materials and methods: To study how Engipore can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed. Results: Engipore causes a significant induction of osteoblast transcriptional factors like SP7 and RUNX2 and of the bone-related gene osteocalcin (BGLAP).The expression of CD105 was not significantly changed in stem cells treated with Engipore with respect to untreated cells, while SSP1 (osteopontin) was significantly down expressed thus reducing osteoclast activity. Conclusions: The obtained results can be relevant to better understand the molecular mechanism of bone regeneration. Keywords: Stem cell, Biomaterial, Engipore, Differentiation, Bone |
| first_indexed | 2024-12-19T12:42:24Z |
| format | Article |
| id | doaj.art-b99f0da72d9542208f4be8bf7b99222c |
| institution | Directory Open Access Journal |
| issn | 1013-9052 |
| language | English |
| last_indexed | 2024-12-19T12:42:24Z |
| publishDate | 2010-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj.art-b99f0da72d9542208f4be8bf7b99222c2022-12-21T20:20:56ZengElsevierSaudi Dental Journal1013-90522010-10-01224161166Engipore acts on human bone marrow stem cellsVincenzo Sollazzo0Annalisa Palmieri1Ambra Girardi2Francesca Farinella3Francesco Carinci4Orthopedic Clinic, University of Ferrara, Corso Giovecca 203, 44100 Ferrara, ItalyDepartment of Maxillofacial Surgery, University of Ferrara, Corso Giovecca 203, 44100 Ferrara, ItalyDepartment of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, 40100 Bologna, ItalyDepartment of Maxillofacial Surgery, University of Ferrara, Corso Giovecca 203, 44100 Ferrara, ItalyDepartment of Maxillofacial Surgery, University of Ferrara, Corso Giovecca 203, 44100 Ferrara, Italy; Corresponding author. Address: Dept. of D.M.C.C.C., University of Ferrara, Corso Giovecca 203, 44100 Ferrara, Italy. Tel./fax: +39 0532 455582.Objectives: Porous HA scaffolds are promising materials for tissue engineering because they offer a tridimensional support and serve as template for cell proliferation and at last tissue formation. Engipore provide a natural 3D scaffold with organic fibrous material in bone. However, how this material alters osteoblast activity to promote bone formation is poorly understood. Materials and methods: To study how Engipore can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed. Results: Engipore causes a significant induction of osteoblast transcriptional factors like SP7 and RUNX2 and of the bone-related gene osteocalcin (BGLAP).The expression of CD105 was not significantly changed in stem cells treated with Engipore with respect to untreated cells, while SSP1 (osteopontin) was significantly down expressed thus reducing osteoclast activity. Conclusions: The obtained results can be relevant to better understand the molecular mechanism of bone regeneration. Keywords: Stem cell, Biomaterial, Engipore, Differentiation, Bonehttp://www.sciencedirect.com/science/article/pii/S1013905210000696 |
| spellingShingle | Vincenzo Sollazzo Annalisa Palmieri Ambra Girardi Francesca Farinella Francesco Carinci Engipore acts on human bone marrow stem cells Saudi Dental Journal |
| title | Engipore acts on human bone marrow stem cells |
| title_full | Engipore acts on human bone marrow stem cells |
| title_fullStr | Engipore acts on human bone marrow stem cells |
| title_full_unstemmed | Engipore acts on human bone marrow stem cells |
| title_short | Engipore acts on human bone marrow stem cells |
| title_sort | engipore acts on human bone marrow stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S1013905210000696 |
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