An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry

Abstract N-glycosylation is an abundant post-translational modification of most cell-surface proteins. N-glycans play a crucial role in cellular functions like protein folding, protein localization, cell–cell signaling, and immune detection. As different tissue types display different N-glycan profi...

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Main Authors: Elizabeth N. Wallace, Connor A. West, Colin T. McDowell, Xiaowei Lu, Evelyn Bruner, Anand S. Mehta, Kiyoko F. Aoki-Kinoshita, Peggi M. Angel, Richard R. Drake
Format: Article
Language:English
Published: Nature Portfolio 2024-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-50957-w
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author Elizabeth N. Wallace
Connor A. West
Colin T. McDowell
Xiaowei Lu
Evelyn Bruner
Anand S. Mehta
Kiyoko F. Aoki-Kinoshita
Peggi M. Angel
Richard R. Drake
author_facet Elizabeth N. Wallace
Connor A. West
Colin T. McDowell
Xiaowei Lu
Evelyn Bruner
Anand S. Mehta
Kiyoko F. Aoki-Kinoshita
Peggi M. Angel
Richard R. Drake
author_sort Elizabeth N. Wallace
collection DOAJ
description Abstract N-glycosylation is an abundant post-translational modification of most cell-surface proteins. N-glycans play a crucial role in cellular functions like protein folding, protein localization, cell–cell signaling, and immune detection. As different tissue types display different N-glycan profiles, changes in N-glycan compositions occur in tissue-specific ways with development of disease, like cancer. However, no comparative atlas resource exists for documenting N-glycome alterations across various human tissue types, particularly comparing normal and cancerous tissues. In order to study a broad range of human tissue N-glycomes, N-glycan targeted MALDI imaging mass spectrometry was applied to custom formalin-fixed paraffin-embedded tissue microarrays. These encompassed fifteen human tissue types including bladder, breast, cervix, colon, esophagus, gastric, kidney, liver, lung, pancreas, prostate, sarcoma, skin, thyroid, and uterus. Each array contained both normal and tumor cores from the same pathology block, selected by a pathologist, allowing more in-depth comparisons of the N-glycome differences between tumor and normal and across tissue types. Using established MALDI-IMS workflows and existing N-glycan databases, the N-glycans present in each tissue core were spatially profiled and peak intensity data compiled for comparative analyses. Further structural information was determined for core fucosylation using endoglycosidase F3, and differentiation of sialic acid linkages through stabilization chemistry. Glycan structural differences across the tissue types were compared for oligomannose levels, branching complexity, presence of bisecting N-acetylglucosamine, fucosylation, and sialylation. Collectively, our research identified the N-glycans that were significantly increased and/or decreased in relative abundance in cancer for each tissue type. This study offers valuable information on a wide scale for both normal and cancerous tissues, serving as a reference for future studies and potential diagnostic applications of MALDI-IMS.
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spelling doaj.art-b99fe5bd597d4068ad3081f8fb25956f2024-01-07T12:25:06ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-023-50957-wAn N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometryElizabeth N. Wallace0Connor A. West1Colin T. McDowell2Xiaowei Lu3Evelyn Bruner4Anand S. Mehta5Kiyoko F. Aoki-Kinoshita6Peggi M. Angel7Richard R. Drake8Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Pathology and Laboratory Medicine, Medical University of South CarolinaDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Bioinformatics, Soka UniversityDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaDepartment of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South CarolinaAbstract N-glycosylation is an abundant post-translational modification of most cell-surface proteins. N-glycans play a crucial role in cellular functions like protein folding, protein localization, cell–cell signaling, and immune detection. As different tissue types display different N-glycan profiles, changes in N-glycan compositions occur in tissue-specific ways with development of disease, like cancer. However, no comparative atlas resource exists for documenting N-glycome alterations across various human tissue types, particularly comparing normal and cancerous tissues. In order to study a broad range of human tissue N-glycomes, N-glycan targeted MALDI imaging mass spectrometry was applied to custom formalin-fixed paraffin-embedded tissue microarrays. These encompassed fifteen human tissue types including bladder, breast, cervix, colon, esophagus, gastric, kidney, liver, lung, pancreas, prostate, sarcoma, skin, thyroid, and uterus. Each array contained both normal and tumor cores from the same pathology block, selected by a pathologist, allowing more in-depth comparisons of the N-glycome differences between tumor and normal and across tissue types. Using established MALDI-IMS workflows and existing N-glycan databases, the N-glycans present in each tissue core were spatially profiled and peak intensity data compiled for comparative analyses. Further structural information was determined for core fucosylation using endoglycosidase F3, and differentiation of sialic acid linkages through stabilization chemistry. Glycan structural differences across the tissue types were compared for oligomannose levels, branching complexity, presence of bisecting N-acetylglucosamine, fucosylation, and sialylation. Collectively, our research identified the N-glycans that were significantly increased and/or decreased in relative abundance in cancer for each tissue type. This study offers valuable information on a wide scale for both normal and cancerous tissues, serving as a reference for future studies and potential diagnostic applications of MALDI-IMS.https://doi.org/10.1038/s41598-023-50957-w
spellingShingle Elizabeth N. Wallace
Connor A. West
Colin T. McDowell
Xiaowei Lu
Evelyn Bruner
Anand S. Mehta
Kiyoko F. Aoki-Kinoshita
Peggi M. Angel
Richard R. Drake
An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
Scientific Reports
title An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
title_full An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
title_fullStr An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
title_full_unstemmed An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
title_short An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
title_sort n glycome tissue atlas of 15 human normal and cancer tissue types determined by maldi imaging mass spectrometry
url https://doi.org/10.1038/s41598-023-50957-w
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