Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology
Traditional protein chips are based on solid chips and cannot enable drug and target screening in a label-free manner. Herein, a protein thermal shift chip (PTSC) based on fluorescence signals is proposed, which enables low-cost, high-throughput, label-free screening. We developed a PTSC for COVID-1...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Compuscript Ltd
2022-08-01
|
| Series: | Acta Materia Medica |
| Subjects: | |
| Online Access: | https://www.scienceopen.com/hosted-document?doi=10.15212/AMM-2022-0016 |
| _version_ | 1827807069704552448 |
|---|---|
| author | Peng Chen Zhao Cui Caifeng Li Shiwen Deng Hongjun Yang |
| author_facet | Peng Chen Zhao Cui Caifeng Li Shiwen Deng Hongjun Yang |
| author_sort | Peng Chen |
| collection | DOAJ |
| description | Traditional protein chips are based on solid chips and cannot enable drug and target screening in a label-free manner. Herein, a protein thermal shift chip (PTSC) based on fluorescence signals is proposed, which enables low-cost, high-throughput, label-free screening. We developed a PTSC for COVID-19, containing 12 SARS-CoV-2 and host target proteins. A series of quality-control tests were performed for small-molecule drugs, macromolecular antibodies, and herbal-medicine extracts. This chip enabled high-throughput screening of COVID-19 drugs and thus may serve as a tool for screening drug targets clinically effective drugs. |
| first_indexed | 2024-03-11T21:51:46Z |
| format | Article |
| id | doaj.art-b9a3b7955f5443948b3d69a5396fe65e |
| institution | Directory Open Access Journal |
| issn | 2737-7946 |
| language | English |
| last_indexed | 2024-03-11T21:51:46Z |
| publishDate | 2022-08-01 |
| publisher | Compuscript Ltd |
| record_format | Article |
| series | Acta Materia Medica |
| spelling | doaj.art-b9a3b7955f5443948b3d69a5396fe65e2023-09-26T08:59:44ZengCompuscript LtdActa Materia Medica2737-79462022-08-011331431910.15212/AMM-2022-0016Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacologyPeng Chen0Zhao Cui1Caifeng Li2Shiwen Deng3Hongjun Yang4Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaTraditional protein chips are based on solid chips and cannot enable drug and target screening in a label-free manner. Herein, a protein thermal shift chip (PTSC) based on fluorescence signals is proposed, which enables low-cost, high-throughput, label-free screening. We developed a PTSC for COVID-19, containing 12 SARS-CoV-2 and host target proteins. A series of quality-control tests were performed for small-molecule drugs, macromolecular antibodies, and herbal-medicine extracts. This chip enabled high-throughput screening of COVID-19 drugs and thus may serve as a tool for screening drug targets clinically effective drugs.https://www.scienceopen.com/hosted-document?doi=10.15212/AMM-2022-0016protein thermal shift chipcovid-19drug targetpharmacology |
| spellingShingle | Peng Chen Zhao Cui Caifeng Li Shiwen Deng Hongjun Yang Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology Acta Materia Medica protein thermal shift chip covid-19 drug target pharmacology |
| title | Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology |
| title_full | Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology |
| title_fullStr | Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology |
| title_full_unstemmed | Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology |
| title_short | Establishment of a protein thermal shift chip (PTSC) for COVID-19 and exploration of the future of protein chips in pharmacology |
| title_sort | establishment of a protein thermal shift chip ptsc for covid 19 and exploration of the future of protein chips in pharmacology |
| topic | protein thermal shift chip covid-19 drug target pharmacology |
| url | https://www.scienceopen.com/hosted-document?doi=10.15212/AMM-2022-0016 |
| work_keys_str_mv | AT pengchen establishmentofaproteinthermalshiftchipptscforcovid19andexplorationofthefutureofproteinchipsinpharmacology AT zhaocui establishmentofaproteinthermalshiftchipptscforcovid19andexplorationofthefutureofproteinchipsinpharmacology AT caifengli establishmentofaproteinthermalshiftchipptscforcovid19andexplorationofthefutureofproteinchipsinpharmacology AT shiwendeng establishmentofaproteinthermalshiftchipptscforcovid19andexplorationofthefutureofproteinchipsinpharmacology AT hongjunyang establishmentofaproteinthermalshiftchipptscforcovid19andexplorationofthefutureofproteinchipsinpharmacology |