A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injurie...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2021-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601&tlng=en |
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| author | T.F.G. Souza T.M. Pierdoná F.S. Macedo P.E.A. Aquino G.F.P. Rangel R.S. Duarte L.M.A. Silva G.S.B. Viana A.P.N.N. Alves R.C. Montenegro D.V. Wilke E.R. Silveira N.M.N. Alencar |
| author_facet | T.F.G. Souza T.M. Pierdoná F.S. Macedo P.E.A. Aquino G.F.P. Rangel R.S. Duarte L.M.A. Silva G.S.B. Viana A.P.N.N. Alves R.C. Montenegro D.V. Wilke E.R. Silveira N.M.N. Alencar |
| author_sort | T.F.G. Souza |
| collection | DOAJ |
| description | It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries. |
| first_indexed | 2024-12-20T15:06:55Z |
| format | Article |
| id | doaj.art-b9a707fd9cda4409bc9c47b62eedcfd5 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-20T15:06:55Z |
| publishDate | 2021-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-b9a707fd9cda4409bc9c47b62eedcfd52022-12-21T19:36:26ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2021-05-0154910.1590/1414-431x2021e10700A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound modelT.F.G. Souzahttps://orcid.org/0000-0002-6530-9768T.M. Pierdonáhttps://orcid.org/0000-0001-6081-6958F.S. Macedohttps://orcid.org/0000-0002-3818-6870P.E.A. Aquinohttps://orcid.org/0000-0002-4756-081XG.F.P. Rangelhttps://orcid.org/0000-0002-7091-8541R.S. Duartehttps://orcid.org/0000-0002-1996-8316L.M.A. Silvahttps://orcid.org/0000-0002-6290-8933G.S.B. Vianahttps://orcid.org/0000-0002-9996-9537A.P.N.N. Alveshttps://orcid.org/0000-0002-5090-6877R.C. Montenegrohttps://orcid.org/0000-0002-3861-293XD.V. Wilkehttps://orcid.org/0000-0001-9481-6702E.R. Silveirahttps://orcid.org/0000-0003-4178-0296N.M.N. Alencarhttps://orcid.org/0000-0001-8340-4876It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601&tlng=enSideroxylon obtusifolium (Roem. & Schult.)Methanol fractionHuman keratinocytesWound healingBurn wounds |
| spellingShingle | T.F.G. Souza T.M. Pierdoná F.S. Macedo P.E.A. Aquino G.F.P. Rangel R.S. Duarte L.M.A. Silva G.S.B. Viana A.P.N.N. Alves R.C. Montenegro D.V. Wilke E.R. Silveira N.M.N. Alencar A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model Brazilian Journal of Medical and Biological Research Sideroxylon obtusifolium (Roem. & Schult.) Methanol fraction Human keratinocytes Wound healing Burn wounds |
| title | A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| title_full | A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| title_fullStr | A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| title_full_unstemmed | A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| title_short | A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| title_sort | proline derivative enriched methanol fraction from sideroxylon obtusifolium leaves mfsol stimulates human keratinocyte cells and exerts a healing effect in a burn wound model |
| topic | Sideroxylon obtusifolium (Roem. & Schult.) Methanol fraction Human keratinocytes Wound healing Burn wounds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000900601&tlng=en |
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