Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components
<p>Abstract</p> <p>Background</p> <p><it>Lychas mucronatus </it>is one scorpion species widely distributed in Southeast Asia and southern China. Anything is hardly known about its venom components, despite the fact that it can often cause human accidents. In...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-07-01
|
| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/452 |
| _version_ | 1818565731083616256 |
|---|---|
| author | Zhijian Cao Yingliang Wu Zhiyong Di Yawen He Yibao Ma Ruiming Zhao Wenxin Li |
| author_facet | Zhijian Cao Yingliang Wu Zhiyong Di Yawen He Yibao Ma Ruiming Zhao Wenxin Li |
| author_sort | Zhijian Cao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Lychas mucronatus </it>is one scorpion species widely distributed in Southeast Asia and southern China. Anything is hardly known about its venom components, despite the fact that it can often cause human accidents. In this work, we performed a venomous gland transcriptome analysis by constructing and screening the venom gland cDNA library of the scorpion <it>Lychas mucronatus </it>from Yunnan province and compared it with the previous results of Hainan-sourced <it>Lychas mucronatus</it>.</p> <p>Results</p> <p>A total of sixteen known types of venom peptides and proteins are obtained from the venom gland cDNA library of Yunnan-sourced <it>Lychas mucronatus</it>, which greatly increase the number of currently reported scorpion venom peptides. Interestingly, we also identified nineteen atypical types of venom molecules seldom reported in scorpion species. Surprisingly, the comparative transcriptome analysis of Yunnan-sourced <it>Lychas mucronatus </it>and Hainan-sourced <it>Lychas mucronatus </it>indicated that enormous diversity and vastly abundant difference could be found in venom peptides and proteins between populations of the scorpion <it>Lychas mucronatus </it>from different geographical regions.</p> <p>Conclusions</p> <p>This work characterizes a large number of venom molecules never identified in scorpion species. This result provides a comparative analysis of venom transcriptomes of the scorpion <it>Lychas mucronatus </it>from different geographical regions, which thoroughly reveals the fact that the venom peptides and proteins of the same scorpion species from different geographical regions are highly diversified and scorpion evolves to adapt a new environment by altering the primary structure and abundance of venom peptides and proteins.</p> |
| first_indexed | 2024-12-14T01:44:53Z |
| format | Article |
| id | doaj.art-b9a8b5764e424681b5a932e30075b933 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T01:44:53Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-b9a8b5764e424681b5a932e30075b9332022-12-21T23:21:36ZengBMCBMC Genomics1471-21642010-07-0111145210.1186/1471-2164-11-452Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous componentsZhijian CaoYingliang WuZhiyong DiYawen HeYibao MaRuiming ZhaoWenxin Li<p>Abstract</p> <p>Background</p> <p><it>Lychas mucronatus </it>is one scorpion species widely distributed in Southeast Asia and southern China. Anything is hardly known about its venom components, despite the fact that it can often cause human accidents. In this work, we performed a venomous gland transcriptome analysis by constructing and screening the venom gland cDNA library of the scorpion <it>Lychas mucronatus </it>from Yunnan province and compared it with the previous results of Hainan-sourced <it>Lychas mucronatus</it>.</p> <p>Results</p> <p>A total of sixteen known types of venom peptides and proteins are obtained from the venom gland cDNA library of Yunnan-sourced <it>Lychas mucronatus</it>, which greatly increase the number of currently reported scorpion venom peptides. Interestingly, we also identified nineteen atypical types of venom molecules seldom reported in scorpion species. Surprisingly, the comparative transcriptome analysis of Yunnan-sourced <it>Lychas mucronatus </it>and Hainan-sourced <it>Lychas mucronatus </it>indicated that enormous diversity and vastly abundant difference could be found in venom peptides and proteins between populations of the scorpion <it>Lychas mucronatus </it>from different geographical regions.</p> <p>Conclusions</p> <p>This work characterizes a large number of venom molecules never identified in scorpion species. This result provides a comparative analysis of venom transcriptomes of the scorpion <it>Lychas mucronatus </it>from different geographical regions, which thoroughly reveals the fact that the venom peptides and proteins of the same scorpion species from different geographical regions are highly diversified and scorpion evolves to adapt a new environment by altering the primary structure and abundance of venom peptides and proteins.</p>http://www.biomedcentral.com/1471-2164/11/452 |
| spellingShingle | Zhijian Cao Yingliang Wu Zhiyong Di Yawen He Yibao Ma Ruiming Zhao Wenxin Li Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components BMC Genomics |
| title | Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components |
| title_full | Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components |
| title_fullStr | Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components |
| title_full_unstemmed | Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components |
| title_short | Comparative venom gland transcriptome analysis of the scorpion <it>Lychas mucronatus </it>reveals intraspecific toxic gene diversity and new venomous components |
| title_sort | comparative venom gland transcriptome analysis of the scorpion it lychas mucronatus it reveals intraspecific toxic gene diversity and new venomous components |
| url | http://www.biomedcentral.com/1471-2164/11/452 |
| work_keys_str_mv | AT zhijiancao comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT yingliangwu comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT zhiyongdi comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT yawenhe comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT yibaoma comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT ruimingzhao comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents AT wenxinli comparativevenomglandtranscriptomeanalysisofthescorpionitlychasmucronatusitrevealsintraspecifictoxicgenediversityandnewvenomouscomponents |