Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets
Piper kadsura is a vine-like medicinal plant which is widely used in clinical treatment. However, P. kadsura is often substituted by other materials in the markets, thereby causing health risks. In this study, 38 P. kadsura samples and eight sequences from GenBank, including a closely-related specie...
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| Format: | Article |
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MDPI AG
2016-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/9/1221 |
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| author | Ning Yu Hong Gu Yulong Wei Ning Zhu Yanli Wang Haiping Zhang Yue Zhu Xin Zhang Chao Ma Aidong Sun |
| author_facet | Ning Yu Hong Gu Yulong Wei Ning Zhu Yanli Wang Haiping Zhang Yue Zhu Xin Zhang Chao Ma Aidong Sun |
| author_sort | Ning Yu |
| collection | DOAJ |
| description | Piper kadsura is a vine-like medicinal plant which is widely used in clinical treatment. However, P. kadsura is often substituted by other materials in the markets, thereby causing health risks. In this study, 38 P. kadsura samples and eight sequences from GenBank, including a closely-related species and common adulterants were collected. This study aimed to identify an effective DNA barcode from four popular DNA loci for P. kadsura authentication. The success rates of PCR amplification, sequencing, and sequence acquisition of matK were 10.5%, 75%, and 7.9%, respectively; for rbcL they were 89.5%, 8.8%, and 7.9%, respectively; ITS2 rates were 86.8%, 3.0%, and 2.6%, respectively, while for psbA-trnH they were all 100%, which is much higher than for the other three loci. The sequences were aligned using Muscle, genetic distances were computed using MEGA 5.2.2, and barcoding gap was performed using TAXON DNA. Phylogenetic analysis showed that psbA-trnH could clearly distinguish P. kadsura from its closely related species and the common adulterant. psbA-trnH was then used to evaluate the fake proportions of P. kadsura. Results showed that 18.4% of P. kadsura samples were fake, indicating that adulterant species exist in the Chinese markets. Two-dimensional DNA barcoding imaging of P. kadsura was conducted, which was beneficial to the management of P. kadsura. We conclude that the psbA-trnH region is a powerful tool for P. kadsura identification and supervision in the current medicine markets. |
| first_indexed | 2024-12-13T18:18:09Z |
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| id | doaj.art-b9a9470cecbc48e8bc29af5ff9a40ff2 |
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| language | English |
| last_indexed | 2024-12-13T18:18:09Z |
| publishDate | 2016-09-01 |
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| series | Molecules |
| spelling | doaj.art-b9a9470cecbc48e8bc29af5ff9a40ff22022-12-21T23:35:47ZengMDPI AGMolecules1420-30492016-09-01219122110.3390/molecules21091221molecules21091221Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine MarketsNing Yu0Hong Gu1Yulong Wei2Ning Zhu3Yanli Wang4Haiping Zhang5Yue Zhu6Xin Zhang7Chao Ma8Aidong Sun9College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaPiper kadsura is a vine-like medicinal plant which is widely used in clinical treatment. However, P. kadsura is often substituted by other materials in the markets, thereby causing health risks. In this study, 38 P. kadsura samples and eight sequences from GenBank, including a closely-related species and common adulterants were collected. This study aimed to identify an effective DNA barcode from four popular DNA loci for P. kadsura authentication. The success rates of PCR amplification, sequencing, and sequence acquisition of matK were 10.5%, 75%, and 7.9%, respectively; for rbcL they were 89.5%, 8.8%, and 7.9%, respectively; ITS2 rates were 86.8%, 3.0%, and 2.6%, respectively, while for psbA-trnH they were all 100%, which is much higher than for the other three loci. The sequences were aligned using Muscle, genetic distances were computed using MEGA 5.2.2, and barcoding gap was performed using TAXON DNA. Phylogenetic analysis showed that psbA-trnH could clearly distinguish P. kadsura from its closely related species and the common adulterant. psbA-trnH was then used to evaluate the fake proportions of P. kadsura. Results showed that 18.4% of P. kadsura samples were fake, indicating that adulterant species exist in the Chinese markets. Two-dimensional DNA barcoding imaging of P. kadsura was conducted, which was beneficial to the management of P. kadsura. We conclude that the psbA-trnH region is a powerful tool for P. kadsura identification and supervision in the current medicine markets.http://www.mdpi.com/1420-3049/21/9/1221Piper kadsuraDNA barcodingpsbA-trnHmedicine market supervision |
| spellingShingle | Ning Yu Hong Gu Yulong Wei Ning Zhu Yanli Wang Haiping Zhang Yue Zhu Xin Zhang Chao Ma Aidong Sun Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets Molecules Piper kadsura DNA barcoding psbA-trnH medicine market supervision |
| title | Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets |
| title_full | Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets |
| title_fullStr | Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets |
| title_full_unstemmed | Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets |
| title_short | Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets |
| title_sort | suitable dna barcoding for identification and supervision of piper kadsura in chinese medicine markets |
| topic | Piper kadsura DNA barcoding psbA-trnH medicine market supervision |
| url | http://www.mdpi.com/1420-3049/21/9/1221 |
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