Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake
Residual feed intake (RFI) is one of the indicators of feed efficiency. To investigate the microbial characteristics and differences in the gastrointestinal tract of beef cattle with different RFI, a metagenome methodology was used to explore the characteristics of the rumen and fecal microbiota in...
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MDPI AG
2023-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/358 |
| _version_ | 1797619182412496896 |
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| author | Xiaonan Zhou Ying Ma Chaoyun Yang Zhiyan Zhao Yanling Ding Yanfeng Zhang Pengfei Wang Lei Zhao Chenglong Li Zonghua Su Xiaowei Wang Wenxun Ming Ling Zeng Xiaolong Kang |
| author_facet | Xiaonan Zhou Ying Ma Chaoyun Yang Zhiyan Zhao Yanling Ding Yanfeng Zhang Pengfei Wang Lei Zhao Chenglong Li Zonghua Su Xiaowei Wang Wenxun Ming Ling Zeng Xiaolong Kang |
| author_sort | Xiaonan Zhou |
| collection | DOAJ |
| description | Residual feed intake (RFI) is one of the indicators of feed efficiency. To investigate the microbial characteristics and differences in the gastrointestinal tract of beef cattle with different RFI, a metagenome methodology was used to explore the characteristics of the rumen and fecal microbiota in 10 Qinchuan cattle (five in each of the extremely high and extremely low RFI groups). The results of taxonomic annotation revealed that <i>Bacteroidetes</i> and <i>Firmicutes</i> were the most dominant phyla in rumen and feces. <i>Prevotella</i> was identified as a potential biomarker in the rumen of the LRFI group by the LEfSe method, while <i>Turicibacter</i> and <i>Prevotella</i> might be potential biomarkers of the HRFI and LRFI group in feces, respectively. Functional annotation revealed that the microbiota in the rumen of the HRFI group had a greater ability to utilize dietary polysaccharides and dietary protein. Association analysis of rumen microbes (genus level) with host genes revealed that microbiota including <i>Prevotella</i>, <i>Paraprevotella</i>, <i>Treponema</i>, <i>Oscillibacter</i>, and <i>Muribaculum</i>, were significantly associated with differentially expressed genes regulating RFI. This study discovered variances in the microbial composition of rumen and feces of beef cattle with different RFIs, demonstrating that differences in microbes may play a critical role in regulating the bovine divergent RFI phenotype variations. |
| first_indexed | 2024-03-11T08:23:26Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T08:23:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b9aab2b20b174491ac300bc74525d3242023-11-16T22:14:46ZengMDPI AGMicroorganisms2076-26072023-01-0111235810.3390/microorganisms11020358Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed IntakeXiaonan Zhou0Ying Ma1Chaoyun Yang2Zhiyan Zhao3Yanling Ding4Yanfeng Zhang5Pengfei Wang6Lei Zhao7Chenglong Li8Zonghua Su9Xiaowei Wang10Wenxun Ming11Ling Zeng12Xiaolong Kang13Key Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaKey Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan 750021, ChinaResidual feed intake (RFI) is one of the indicators of feed efficiency. To investigate the microbial characteristics and differences in the gastrointestinal tract of beef cattle with different RFI, a metagenome methodology was used to explore the characteristics of the rumen and fecal microbiota in 10 Qinchuan cattle (five in each of the extremely high and extremely low RFI groups). The results of taxonomic annotation revealed that <i>Bacteroidetes</i> and <i>Firmicutes</i> were the most dominant phyla in rumen and feces. <i>Prevotella</i> was identified as a potential biomarker in the rumen of the LRFI group by the LEfSe method, while <i>Turicibacter</i> and <i>Prevotella</i> might be potential biomarkers of the HRFI and LRFI group in feces, respectively. Functional annotation revealed that the microbiota in the rumen of the HRFI group had a greater ability to utilize dietary polysaccharides and dietary protein. Association analysis of rumen microbes (genus level) with host genes revealed that microbiota including <i>Prevotella</i>, <i>Paraprevotella</i>, <i>Treponema</i>, <i>Oscillibacter</i>, and <i>Muribaculum</i>, were significantly associated with differentially expressed genes regulating RFI. This study discovered variances in the microbial composition of rumen and feces of beef cattle with different RFIs, demonstrating that differences in microbes may play a critical role in regulating the bovine divergent RFI phenotype variations.https://www.mdpi.com/2076-2607/11/2/358metagenomebeef cattlemicrobiotarumen and manureresidual feed intake |
| spellingShingle | Xiaonan Zhou Ying Ma Chaoyun Yang Zhiyan Zhao Yanling Ding Yanfeng Zhang Pengfei Wang Lei Zhao Chenglong Li Zonghua Su Xiaowei Wang Wenxun Ming Ling Zeng Xiaolong Kang Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake Microorganisms metagenome beef cattle microbiota rumen and manure residual feed intake |
| title | Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake |
| title_full | Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake |
| title_fullStr | Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake |
| title_full_unstemmed | Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake |
| title_short | Rumen and Fecal Microbiota Characteristics of Qinchuan Cattle with Divergent Residual Feed Intake |
| title_sort | rumen and fecal microbiota characteristics of qinchuan cattle with divergent residual feed intake |
| topic | metagenome beef cattle microbiota rumen and manure residual feed intake |
| url | https://www.mdpi.com/2076-2607/11/2/358 |
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