Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia
In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2016-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005001104&lng=en&tlng=en |
| _version_ | 1827833102820442112 |
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| author | Carolina Bioni Garcia Teles Ana Paula de Azevedo dos Santos Rui Alves Freitas Arley Faria José de Oliveira Guilherme Maerschner Ogawa Moreno Souza Rodrigues Felipe Arley Costa Pessoa Jansen Fernandes Medeiros Luís Marcelo Aranha Camargo |
| author_facet | Carolina Bioni Garcia Teles Ana Paula de Azevedo dos Santos Rui Alves Freitas Arley Faria José de Oliveira Guilherme Maerschner Ogawa Moreno Souza Rodrigues Felipe Arley Costa Pessoa Jansen Fernandes Medeiros Luís Marcelo Aranha Camargo |
| author_sort | Carolina Bioni Garcia Teles |
| collection | DOAJ |
| description | In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality. |
| first_indexed | 2024-03-12T05:25:02Z |
| format | Article |
| id | doaj.art-b9b0ab8d471641aa926fec63559a847b |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:02Z |
| publishDate | 2016-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-b9b0ab8d471641aa926fec63559a847b2023-09-03T07:25:02ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602016-01-01010.1590/0074-02760160054S0074-02762016005001104Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and BoliviaCarolina Bioni Garcia TelesAna Paula de Azevedo dos SantosRui Alves FreitasArley Faria José de OliveiraGuilherme Maerschner OgawaMoreno Souza RodriguesFelipe Arley Costa PessoaJansen Fernandes MedeirosLuís Marcelo Aranha CamargoIn this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005001104&lng=en&tlng=ensandfliesLeishmaniadisease vectorsAssis Brasil MunicipalityAcreBrazil |
| spellingShingle | Carolina Bioni Garcia Teles Ana Paula de Azevedo dos Santos Rui Alves Freitas Arley Faria José de Oliveira Guilherme Maerschner Ogawa Moreno Souza Rodrigues Felipe Arley Costa Pessoa Jansen Fernandes Medeiros Luís Marcelo Aranha Camargo Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia Memorias do Instituto Oswaldo Cruz sandflies Leishmania disease vectors Assis Brasil Municipality Acre Brazil |
| title | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
| title_full | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
| title_fullStr | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
| title_full_unstemmed | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
| title_short | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
| title_sort | phlebotomine sandfly diptera psychodidae diversity and their leishmania dna in a hot spot of american cutaneous leishmaniasis human cases along the brazilian border with peru and bolivia |
| topic | sandflies Leishmania disease vectors Assis Brasil Municipality Acre Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005001104&lng=en&tlng=en |
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