Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen
To preserve genetic materials, cryopreservation of the semen from live animals is the main technique to establish cryo-banking system which could be used for artificial insemination and embryo transfer. However, the population of Korean black goat (KBG) becomes to dwindle in number and is now faced...
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| Format: | Article |
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The Korean Society of Animal Reproduction and Biotechnology
2017-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=1584&vmd=Full |
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| author | Sung Woo Kim Jinwook Lee Kwan-woo Kim Chan-Lan Kim Ik Soo Jeon Sung-soo Lee |
| author_facet | Sung Woo Kim Jinwook Lee Kwan-woo Kim Chan-Lan Kim Ik Soo Jeon Sung-soo Lee |
| author_sort | Sung Woo Kim |
| collection | DOAJ |
| description | To preserve genetic materials, cryopreservation of the semen from live animals is the main technique to establish cryo-banking system which could be used for artificial insemination and embryo transfer. However, the population of Korean black goat (KBG) becomes to dwindle in number and is now faced genetic erosion by crossbreeding with non-native breeds in small KBG farms. In this study, simple freezing method was used to preserve frozen semen from KBG using spermatozoa of cauda epididymis (CE) and electro-stimulated semen (ES). The negative effects of seminal plasma on fresh sperm was confirmed using precipitation test of Triladyl egg yolk diluent and sperm viability after thawing was compared between CE and ES spermatozoa. When seminal plasma of fresh ES semen was washed with semen washing media (SWM), the rates of live sperm shown no significant difference between CE and ES spermatozoa before freezing. However, the survival rate of frozen/thawed CE sperm was higher than ES (74.6±10.6% vs 53.8±5.2%) with significant difference (p < 0.05). The results of longevity test on frozen/thawed sperm from CE showed healthier sperm than ES. Therefore, spermatozoa from CE could be used for cryo-banking system in KBG lines. The more studies are needed to increase survival rate of ES semen. |
| first_indexed | 2024-12-16T16:02:22Z |
| format | Article |
| id | doaj.art-b9b28ef6819143c88a3b13949b57bead |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-16T16:02:22Z |
| publishDate | 2017-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-b9b28ef6819143c88a3b13949b57bead2022-12-21T22:25:26ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632017-09-0132323524110.12750/JET.2017.32.3.235Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated SemenSung Woo Kim0Jinwook Lee1Kwan-woo Kim2Chan-Lan Kim3Ik Soo Jeon4Sung-soo Lee5Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoTo preserve genetic materials, cryopreservation of the semen from live animals is the main technique to establish cryo-banking system which could be used for artificial insemination and embryo transfer. However, the population of Korean black goat (KBG) becomes to dwindle in number and is now faced genetic erosion by crossbreeding with non-native breeds in small KBG farms. In this study, simple freezing method was used to preserve frozen semen from KBG using spermatozoa of cauda epididymis (CE) and electro-stimulated semen (ES). The negative effects of seminal plasma on fresh sperm was confirmed using precipitation test of Triladyl egg yolk diluent and sperm viability after thawing was compared between CE and ES spermatozoa. When seminal plasma of fresh ES semen was washed with semen washing media (SWM), the rates of live sperm shown no significant difference between CE and ES spermatozoa before freezing. However, the survival rate of frozen/thawed CE sperm was higher than ES (74.6±10.6% vs 53.8±5.2%) with significant difference (p < 0.05). The results of longevity test on frozen/thawed sperm from CE showed healthier sperm than ES. Therefore, spermatozoa from CE could be used for cryo-banking system in KBG lines. The more studies are needed to increase survival rate of ES semen.http://www.e-jarb.org/journal/view.html?uid=1584&vmd=Fullkorean black goatcauda epididymissemenelectro-ejaculation |
| spellingShingle | Sung Woo Kim Jinwook Lee Kwan-woo Kim Chan-Lan Kim Ik Soo Jeon Sung-soo Lee Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen Journal of Animal Reproduction and Biotechnology korean black goat cauda epididymis semen electro-ejaculation |
| title | Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen |
| title_full | Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen |
| title_fullStr | Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen |
| title_full_unstemmed | Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen |
| title_short | Effects of Triladyl-egg Yolk Diluents on the Viability of Frozen Korean Blackgoat Spermatozoa from Cauda Epididymis and Electro-ejaculated Semen |
| title_sort | effects of triladyl egg yolk diluents on the viability of frozen korean blackgoat spermatozoa from cauda epididymis and electro ejaculated semen |
| topic | korean black goat cauda epididymis semen electro-ejaculation |
| url | http://www.e-jarb.org/journal/view.html?uid=1584&vmd=Full |
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