Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection
Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application;...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/4/936 |
| _version_ | 1811302701885030400 |
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| author | Lisa Christadore Mark W. Grinstaff Scott E. Schaus |
| author_facet | Lisa Christadore Mark W. Grinstaff Scott E. Schaus |
| author_sort | Lisa Christadore |
| collection | DOAJ |
| description | Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available. |
| first_indexed | 2024-04-13T07:33:53Z |
| format | Article |
| id | doaj.art-b9b2c12178564892ac7c5ef21f073317 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T07:33:53Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b9b2c12178564892ac7c5ef21f0733172022-12-22T02:56:15ZengMDPI AGMolecules1420-30492018-04-0123493610.3390/molecules23040936molecules23040936Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell DetectionLisa Christadore0Mark W. Grinstaff1Scott E. Schaus2Department of Chemistry, Boston University, Boston, MA 02215, USADepartment of Chemistry, Boston University, Boston, MA 02215, USADepartment of Chemistry, Boston University, Boston, MA 02215, USAHydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available.http://www.mdpi.com/1420-3049/23/4/936dendrimerhydrogelscreeningcrosslinkingfluorescence |
| spellingShingle | Lisa Christadore Mark W. Grinstaff Scott E. Schaus Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection Molecules dendrimer hydrogel screening crosslinking fluorescence |
| title | Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection |
| title_full | Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection |
| title_fullStr | Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection |
| title_full_unstemmed | Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection |
| title_short | Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection |
| title_sort | fluorescent dendritic micro hydrogels synthesis analysis and use in single cell detection |
| topic | dendrimer hydrogel screening crosslinking fluorescence |
| url | http://www.mdpi.com/1420-3049/23/4/936 |
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