Assessment of Salt Stress to <i>Arabidopsis</i> Based on the Detection of Hydrogen Peroxide Released by Leaves Using an Electrochemical Sensor
Salt stress will have a serious inhibitory effect on various metabolic processes of plant cells, this will lead to the excessive accumulation of reactive oxygen species (ROS). Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is a type of ROS that can severely damage plant cells...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/20/12502 |
| Summary: | Salt stress will have a serious inhibitory effect on various metabolic processes of plant cells, this will lead to the excessive accumulation of reactive oxygen species (ROS). Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is a type of ROS that can severely damage plant cells in large amounts. Existing methods for assessing the content of H<sub>2</sub>O<sub>2</sub> released from leaves under salt stress will cause irreversible damage to plant leaves and are unable to detect H<sub>2</sub>O<sub>2</sub> production in real time. In this study, on the strength of a series of physiological indicators to verify the occurrence of salt stress, an electrochemical sensor for the detection of H<sub>2</sub>O<sub>2</sub> released from leaves under salt stress was constructed. The sensor was prepared by using multi-walled carbon nanotube-titanium carbide–palladium (MWCNT-Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>-Pd) nanocomposite as substrate material and showed a linear response to H<sub>2</sub>O<sub>2</sub> detection in the range 0.05–18 mM with a detection limit of 3.83 μM. Moreover, we measured the determination of H<sub>2</sub>O<sub>2</sub> released from <i>Arabidopsis</i> leaves at different times of salt stress by the sensor, which was consistent with conventional method. This study demonstrates that electrochemical sensing is a desirable technology for the dynamic determination of H<sub>2</sub>O<sub>2</sub> released by leaves and the assessment of salt stress to plants. |
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| ISSN: | 1661-6596 1422-0067 |