Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage
Background/Aims Sepsis is an uncontrolled systemic infection, withcomplex pathophysiology that may result in acute lung organ damage and cause multiple organ failure. Although much research has been conducted to illuminate sepsis’s complex pathophysiology, sepsis treatment protocols are limited, and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-02-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2021.1898063 |
| _version_ | 1827817161553346560 |
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| author | Erol Akpinar Zerrin Kutlu Duygu Kose Pelin Aydin Taha Tavaci Zafer Bayraktutan Tugba Nurcan Yuksel Serkan Yildirim Gizem Eser Busra Dincer |
| author_facet | Erol Akpinar Zerrin Kutlu Duygu Kose Pelin Aydin Taha Tavaci Zafer Bayraktutan Tugba Nurcan Yuksel Serkan Yildirim Gizem Eser Busra Dincer |
| author_sort | Erol Akpinar |
| collection | DOAJ |
| description | Background/Aims Sepsis is an uncontrolled systemic infection, withcomplex pathophysiology that may result in acute lung organ damage and cause multiple organ failure. Although much research has been conducted to illuminate sepsis’s complex pathophysiology, sepsis treatment protocols are limited, and sepsis remains an important cause of mortality andmorbidity in intensive care units.Various studies have shown that idebenone (IDE) possesses strong antioxidant properties, which inhibit lipid peroxidation and protect cells from oxidative damage. The present study aimed to evaluate the protective effects of IDE against lung injury in a cecal ligation and puncture (CLP)-induced sepsis rat model. Methods Male albino Wistar rats were used. The animals were divided into a healthy control (no treatment), CLP, IDE control (200 mg/kg), and CLP + IDE subgroups (50 mg/kg, 100 mg/kg, and 200 mg/kg), with nine rats in each group.IDE was administered 1 h after CLP induction.To evaluate the protective effects of IDE, lung tissues were collected 16 h after sepsis for biochemical, immunohistochemical staining, and histopathological examination. Results IDE significantly ameliorated sepsis-induced disturbances in oxidative stress-related factors, with its effects increasing in accordance with the dose.IDE also abolished histopathological changes in lung tissues associated with CLP.Furthermore, interleukin 1 beta (IL-1β)and tumor necrosis factor-alpha (TNF-α) immunopositivity markedly decreased in the septic rats following IDE treatment. Conclusions IDE largely mitigated the inflammatory response in sepsis-induced lung injury by decreasing free radicals and preventing lipid peroxidation. The results suggest that IDE may represent a potential novel therapeutic drug for sepsis treatment. |
| first_indexed | 2024-03-12T00:30:28Z |
| format | Article |
| id | doaj.art-b9b33cd9727e41f3a926867737f342d5 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:30:28Z |
| publishDate | 2022-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-b9b33cd9727e41f3a926867737f342d52023-09-15T10:21:28ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532022-02-0135356056810.1080/08941939.2021.18980631898063Protective Effects of Idebenone against Sepsis Induced Acute Lung DamageErol Akpinar0Zerrin Kutlu1Duygu Kose2Pelin Aydin3Taha Tavaci4Zafer Bayraktutan5Tugba Nurcan Yuksel6Serkan Yildirim7Gizem Eser8Busra Dincer9Department of Pharmacology, Faculty of Medicine, Ataturk UniversityDepartment of Biochemistry, Faculty of Pharmacy, Ataturk UniversityDepartment of Pharmacology, Faculty of Medicine, Ataturk UniversityDepartment of Pharmacology, Faculty of Medicine, Ataturk UniversityDepartment of Pharmacology, Faculty of Medicine, Ataturk UniversityDepartment of Biochemistry, Faculty of Medicine, Ataturk UniversityDepartment of Pharmacology, Faculty of Medicine, Namik Kemal UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Ataturk UniversityDepartment of Pathology, Faculty of Veterinary Medicine, Ataturk UniversityDepartment of Pharmacology, Faculty of Pharmacy, Erzincan Binali Yildirim UniversityBackground/Aims Sepsis is an uncontrolled systemic infection, withcomplex pathophysiology that may result in acute lung organ damage and cause multiple organ failure. Although much research has been conducted to illuminate sepsis’s complex pathophysiology, sepsis treatment protocols are limited, and sepsis remains an important cause of mortality andmorbidity in intensive care units.Various studies have shown that idebenone (IDE) possesses strong antioxidant properties, which inhibit lipid peroxidation and protect cells from oxidative damage. The present study aimed to evaluate the protective effects of IDE against lung injury in a cecal ligation and puncture (CLP)-induced sepsis rat model. Methods Male albino Wistar rats were used. The animals were divided into a healthy control (no treatment), CLP, IDE control (200 mg/kg), and CLP + IDE subgroups (50 mg/kg, 100 mg/kg, and 200 mg/kg), with nine rats in each group.IDE was administered 1 h after CLP induction.To evaluate the protective effects of IDE, lung tissues were collected 16 h after sepsis for biochemical, immunohistochemical staining, and histopathological examination. Results IDE significantly ameliorated sepsis-induced disturbances in oxidative stress-related factors, with its effects increasing in accordance with the dose.IDE also abolished histopathological changes in lung tissues associated with CLP.Furthermore, interleukin 1 beta (IL-1β)and tumor necrosis factor-alpha (TNF-α) immunopositivity markedly decreased in the septic rats following IDE treatment. Conclusions IDE largely mitigated the inflammatory response in sepsis-induced lung injury by decreasing free radicals and preventing lipid peroxidation. The results suggest that IDE may represent a potential novel therapeutic drug for sepsis treatment.http://dx.doi.org/10.1080/08941939.2021.1898063idebenonesepsisantioxidantlung injuryratoxidative stress |
| spellingShingle | Erol Akpinar Zerrin Kutlu Duygu Kose Pelin Aydin Taha Tavaci Zafer Bayraktutan Tugba Nurcan Yuksel Serkan Yildirim Gizem Eser Busra Dincer Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage Journal of Investigative Surgery idebenone sepsis antioxidantlung injuryrat oxidative stress |
| title | Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage |
| title_full | Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage |
| title_fullStr | Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage |
| title_full_unstemmed | Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage |
| title_short | Protective Effects of Idebenone against Sepsis Induced Acute Lung Damage |
| title_sort | protective effects of idebenone against sepsis induced acute lung damage |
| topic | idebenone sepsis antioxidantlung injuryrat oxidative stress |
| url | http://dx.doi.org/10.1080/08941939.2021.1898063 |
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