Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
<p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide ran...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2008-04-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/7/1/12 |
| _version_ | 1818261776235495424 |
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| author | Tuma Roman Suchanova Bohumila |
| author_facet | Tuma Roman Suchanova Bohumila |
| author_sort | Tuma Roman |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.</p> |
| first_indexed | 2024-12-12T18:52:36Z |
| format | Article |
| id | doaj.art-b9b531cb651d49cf8263273d0a5c1f55 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-12T18:52:36Z |
| publishDate | 2008-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-b9b531cb651d49cf8263273d0a5c1f552022-12-22T00:15:20ZengBMCMicrobial Cell Factories1475-28592008-04-01711210.1186/1475-2859-7-12Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometryTuma RomanSuchanova Bohumila<p>Abstract</p> <p>Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.</p>http://www.microbialcellfactories.com/content/7/1/12 |
| spellingShingle | Tuma Roman Suchanova Bohumila Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry Microbial Cell Factories |
| title | Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry |
| title_full | Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry |
| title_fullStr | Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry |
| title_full_unstemmed | Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry |
| title_short | Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry |
| title_sort | folding and assembly of large macromolecular complexes monitored by hydrogen deuterium exchange and mass spectrometry |
| url | http://www.microbialcellfactories.com/content/7/1/12 |
| work_keys_str_mv | AT tumaroman foldingandassemblyoflargemacromolecularcomplexesmonitoredbyhydrogendeuteriumexchangeandmassspectrometry AT suchanovabohumila foldingandassemblyoflargemacromolecularcomplexesmonitoredbyhydrogendeuteriumexchangeandmassspectrometry |