Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome
The renal system is engaged in metabolic syndrome (MS) and metabolites of arachidonic acid (AA) participate in renal homeostasis and disruption of functionality. <i>Hibiscus sabdariffa</i> L (HSL) is used as a diuretic and could improve renal function. The aim of this study was to assess...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14209 |
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| author | Israel Pérez-Torres María Elena Soto Linaloe Manzano-Pech Eulises Díaz-Díaz Raúl Martínez-Memije Juan Carlos Torres-Narváez Verónica Guarner-Lans Vicente Castrejón-Téllez |
| author_facet | Israel Pérez-Torres María Elena Soto Linaloe Manzano-Pech Eulises Díaz-Díaz Raúl Martínez-Memije Juan Carlos Torres-Narváez Verónica Guarner-Lans Vicente Castrejón-Téllez |
| author_sort | Israel Pérez-Torres |
| collection | DOAJ |
| description | The renal system is engaged in metabolic syndrome (MS) and metabolites of arachidonic acid (AA) participate in renal homeostasis and disruption of functionality. <i>Hibiscus sabdariffa</i> L (HSL) is used as a diuretic and could improve renal function. The aim of this study was to assess if treatment with HSL at 2% improves renal function in MS through the metabolites of AA. A total of 24 male Wistar rats were divided into four groups: Group 1, control (C); Group 2, MS with 30% sucrose in drinking water, Group 3, MS plus HSL infusion at 2% (MS+HSL); and Group 4, C+HSL. We evaluated the perfusion pressure changes (∆-PP), the activities of cyclooxygenases (COXs), the percentage of AA, the expressions of PLA<sub>2</sub>, COX2, COX1, 5-LOX, TAXS and CYP450, and the concentrations of prostaglandins in the kidney from rats with MS. There was a decrease in the ∆-PP, in the activities of COXs, and the expressions of COX2 and CYP450 (<i>p</i> ≤ 0.03, respectively)as well asPGE<sub>2</sub>, TxB<sub>2</sub>, and LKB<sub>4</sub> (<i>p</i> ≤ 0.01, respectively). However, the percentage of AA and expressions of PLA<sub>2</sub> and PGE1 (<i>p</i> = 0.01, respectively) were increased in C and MS+HSL. The HSL treatment improved the function and anatomical structure of the kidneys in the MS rats, through antioxidant molecules, and inhibited the pathways that metabolize the AA including that of PLA<sub>2</sub>, COX2, 5-LOX, TAXS, and CYP450 while favoring the COX1 pathway. This improves the vascular resistance of renal arterioles. |
| first_indexed | 2024-03-10T22:39:33Z |
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| id | doaj.art-b9b81138e72f4da29f936031ce0ce176 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:39:33Z |
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| spelling | doaj.art-b9b81138e72f4da29f936031ce0ce1762023-11-19T11:09:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181420910.3390/ijms241814209Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic SyndromeIsrael Pérez-Torres0María Elena Soto1Linaloe Manzano-Pech2Eulises Díaz-Díaz3Raúl Martínez-Memije4Juan Carlos Torres-Narváez5Verónica Guarner-Lans6Vicente Castrejón-Téllez7Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoDepartment of Immunology and Research Direction, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoDepartment of Cardiovascular Biomedicine, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoDepartment of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Sección XVI, Tlalpan, México City 14000, MexicoDepartment de Instrumentation Electromechanically, Instituto Nacional de Cardiología Ignacio Chávez, Tlalpan, México City 14080, MexicoDepartment of Pharmacology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoDepartment of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoDepartment of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, México City 14080, MexicoThe renal system is engaged in metabolic syndrome (MS) and metabolites of arachidonic acid (AA) participate in renal homeostasis and disruption of functionality. <i>Hibiscus sabdariffa</i> L (HSL) is used as a diuretic and could improve renal function. The aim of this study was to assess if treatment with HSL at 2% improves renal function in MS through the metabolites of AA. A total of 24 male Wistar rats were divided into four groups: Group 1, control (C); Group 2, MS with 30% sucrose in drinking water, Group 3, MS plus HSL infusion at 2% (MS+HSL); and Group 4, C+HSL. We evaluated the perfusion pressure changes (∆-PP), the activities of cyclooxygenases (COXs), the percentage of AA, the expressions of PLA<sub>2</sub>, COX2, COX1, 5-LOX, TAXS and CYP450, and the concentrations of prostaglandins in the kidney from rats with MS. There was a decrease in the ∆-PP, in the activities of COXs, and the expressions of COX2 and CYP450 (<i>p</i> ≤ 0.03, respectively)as well asPGE<sub>2</sub>, TxB<sub>2</sub>, and LKB<sub>4</sub> (<i>p</i> ≤ 0.01, respectively). However, the percentage of AA and expressions of PLA<sub>2</sub> and PGE1 (<i>p</i> = 0.01, respectively) were increased in C and MS+HSL. The HSL treatment improved the function and anatomical structure of the kidneys in the MS rats, through antioxidant molecules, and inhibited the pathways that metabolize the AA including that of PLA<sub>2</sub>, COX2, 5-LOX, TAXS, and CYP450 while favoring the COX1 pathway. This improves the vascular resistance of renal arterioles.https://www.mdpi.com/1422-0067/24/18/14209<i>Hibiscus sabdariffa</i> L.arachidonic acidcyclooxygenase metabolismmetabolic syndromekidney |
| spellingShingle | Israel Pérez-Torres María Elena Soto Linaloe Manzano-Pech Eulises Díaz-Díaz Raúl Martínez-Memije Juan Carlos Torres-Narváez Verónica Guarner-Lans Vicente Castrejón-Téllez Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome International Journal of Molecular Sciences <i>Hibiscus sabdariffa</i> L. arachidonic acid cyclooxygenase metabolism metabolic syndrome kidney |
| title | Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome |
| title_full | Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome |
| title_fullStr | Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome |
| title_full_unstemmed | Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome |
| title_short | Effect of <i>Hibiscus sabdariffa</i> L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome |
| title_sort | effect of i hibiscus sabdariffa i l on the metabolism of arachidonic acid in the isolated kidney of a rat model of metabolic syndrome |
| topic | <i>Hibiscus sabdariffa</i> L. arachidonic acid cyclooxygenase metabolism metabolic syndrome kidney |
| url | https://www.mdpi.com/1422-0067/24/18/14209 |
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