New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata
In this study, we report soft ticks from bat-inhabiting caves in different areas of Brazil. From 2010 to 2019, we collected 807 tick specimens from nine caves located in four Brazilian states among two biomes. Ticks were morphologically identified as Antricola guglielmonei (282 specimens), Ornithodo...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X24000244 |
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| author | Glauber M.B. de Oliveira Sebastián Muñoz-Leal Santiago Nava Maurício C. Horta Leopoldo Bernardi José Manuel Venzal Marcelo B. Labruna |
| author_facet | Glauber M.B. de Oliveira Sebastián Muñoz-Leal Santiago Nava Maurício C. Horta Leopoldo Bernardi José Manuel Venzal Marcelo B. Labruna |
| author_sort | Glauber M.B. de Oliveira |
| collection | DOAJ |
| description | In this study, we report soft ticks from bat-inhabiting caves in different areas of Brazil. From 2010 to 2019, we collected 807 tick specimens from nine caves located in four Brazilian states among two biomes. Ticks were morphologically identified as Antricola guglielmonei (282 specimens), Ornithodoros cavernicolous (260 specimens), and Ornithodoros fonsecai (265 specimens). Whereas A. guglielmonei was collected on bat guano in hot caves, O. cavernicolous and O. fonsecai were collected in cracks and crevices on the walls of cold caves, sometimes in the same chamber. Morphological identifications were corroborated by molecular and phylogenetic analyses inferred from tick mitochondrial 16S rRNA gene partial sequences. The sequences of A. guglielmonei, O. cavernicolous and O. fonsecai collected in this study clustered with conspecific GenBank sequences from different localities of Brazil. Remarkably, a clade containing 12 sequences of O. fonsecai was clearly bifurcated, denoting a degree of genetic divergence (up to 5 %) of specimens from Cerrado/Atlantic Forest biomes with the specimens from the Caatinga biome. To further evaluate this divergence, we performed morphometric analysis of the larval stage of different O. fonsencai populations by principal component analysis, which indicated that the larvae from Caatinga populations were generally smaller than the larvae from other biomes. Some of the present A. guglielmonei specimens were collected from the type locality of Antricola inexpectata. Comparisons of these specimens with the type specimens of A. inexpectata and A. guglielmonei indicated that they could not be separated by their external morphology. Hence, we are relegating A. inexpectata to a synonym of A. guglielmonei. This proposal is corroborated by our phylogenetic analysis. |
| first_indexed | 2024-04-24T20:26:12Z |
| format | Article |
| id | doaj.art-b9ba4f46b9c84ab684c33eafbf8e1e71 |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-04-24T20:26:12Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-b9ba4f46b9c84ab684c33eafbf8e1e712024-03-22T05:39:05ZengElsevierTicks and Tick-Borne Diseases1877-96032024-05-01153102331New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectataGlauber M.B. de Oliveira0Sebastián Muñoz-Leal1Santiago Nava2Maurício C. Horta3Leopoldo Bernardi4José Manuel Venzal5Marcelo B. Labruna6Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilDepartamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Ñuble, ChileInstituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET), Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela (INTA E.E.A. Rafaela), Rafaela, Santa Fe, ArgentinaLaboratório de Doenças Parasitárias, Universidade Federal do Vale do São Francisco, Petrolina, PE, BrazilDepartamento de Entomologia, Universidade Federal de Lavras, Lavras, MG, BrazilDepartamento de Ciencias Biológicas, CENUR Litoral Norte - Salto, Universidad de la República, Salto, UruguayDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil; Corresponding author.In this study, we report soft ticks from bat-inhabiting caves in different areas of Brazil. From 2010 to 2019, we collected 807 tick specimens from nine caves located in four Brazilian states among two biomes. Ticks were morphologically identified as Antricola guglielmonei (282 specimens), Ornithodoros cavernicolous (260 specimens), and Ornithodoros fonsecai (265 specimens). Whereas A. guglielmonei was collected on bat guano in hot caves, O. cavernicolous and O. fonsecai were collected in cracks and crevices on the walls of cold caves, sometimes in the same chamber. Morphological identifications were corroborated by molecular and phylogenetic analyses inferred from tick mitochondrial 16S rRNA gene partial sequences. The sequences of A. guglielmonei, O. cavernicolous and O. fonsecai collected in this study clustered with conspecific GenBank sequences from different localities of Brazil. Remarkably, a clade containing 12 sequences of O. fonsecai was clearly bifurcated, denoting a degree of genetic divergence (up to 5 %) of specimens from Cerrado/Atlantic Forest biomes with the specimens from the Caatinga biome. To further evaluate this divergence, we performed morphometric analysis of the larval stage of different O. fonsencai populations by principal component analysis, which indicated that the larvae from Caatinga populations were generally smaller than the larvae from other biomes. Some of the present A. guglielmonei specimens were collected from the type locality of Antricola inexpectata. Comparisons of these specimens with the type specimens of A. inexpectata and A. guglielmonei indicated that they could not be separated by their external morphology. Hence, we are relegating A. inexpectata to a synonym of A. guglielmonei. This proposal is corroborated by our phylogenetic analysis.http://www.sciencedirect.com/science/article/pii/S1877959X24000244ArgasidsOrnithodoros cavernicolousAntricola guglielmoneiMorphometryPrincipal component analysisCaatinga |
| spellingShingle | Glauber M.B. de Oliveira Sebastián Muñoz-Leal Santiago Nava Maurício C. Horta Leopoldo Bernardi José Manuel Venzal Marcelo B. Labruna New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata Ticks and Tick-Borne Diseases Argasids Ornithodoros cavernicolous Antricola guglielmonei Morphometry Principal component analysis Caatinga |
| title | New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata |
| title_full | New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata |
| title_fullStr | New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata |
| title_full_unstemmed | New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata |
| title_short | New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata |
| title_sort | new records of soft ticks acari argasidae from caves in brazil with a morphological study of ornithodoros fonsecai and an analysis of the taxonomic status of antricola inexpectata |
| topic | Argasids Ornithodoros cavernicolous Antricola guglielmonei Morphometry Principal component analysis Caatinga |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X24000244 |
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