The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates
The rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 exhibits antagonism toward the phytopathogenic fungus <i>Rosellinia necatrix</i>. This strain efficiently colonizes <i>R. necatrix</i> hyphae and is able to feed on their exudates. Here, we report the comple...
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1388 |
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| author | Adrián Pintado Isabel Pérez-Martínez Isabel M. Aragón José Antonio Gutiérrez-Barranquero Antonio de Vicente Francisco M. Cazorla Cayo Ramos |
| author_facet | Adrián Pintado Isabel Pérez-Martínez Isabel M. Aragón José Antonio Gutiérrez-Barranquero Antonio de Vicente Francisco M. Cazorla Cayo Ramos |
| author_sort | Adrián Pintado |
| collection | DOAJ |
| description | The rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 exhibits antagonism toward the phytopathogenic fungus <i>Rosellinia necatrix</i>. This strain efficiently colonizes <i>R. necatrix</i> hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of <i>P. alcaligenes</i> AVO110. The phylogeny of all available <i>P. alcaligenes</i> genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with <i>Pseudomonas otitidis</i>. Core and pan-genome analyses showed that <i>P. alcaligenes</i> strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by <i>R. necatrix</i> exudates. One of these genes (<i>cmpA</i>) encodes a GGDEF/EAL domain protein specific to <i>Pseudomonas</i> spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of <i>P. alcaligenes</i>, including the mycophagous behavior of strain AVO110. |
| first_indexed | 2024-03-10T10:02:55Z |
| format | Article |
| id | doaj.art-b9be980c27854141a67e396a6ad015a4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:02:55Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b9be980c27854141a67e396a6ad015a42023-11-22T01:49:55ZengMDPI AGMicroorganisms2076-26072021-06-0197138810.3390/microorganisms9071388The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> ExudatesAdrián Pintado0Isabel Pérez-Martínez1Isabel M. Aragón2José Antonio Gutiérrez-Barranquero3Antonio de Vicente4Francisco M. Cazorla5Cayo Ramos6Área de Genética, Facultad de Ciencias, Campus Teatinos, Universidad de Málaga, E-29010 Málaga, SpainÁrea de Genética, Facultad de Ciencias, Campus Teatinos, Universidad de Málaga, E-29010 Málaga, SpainÁrea de Genética, Facultad de Ciencias, Campus Teatinos, Universidad de Málaga, E-29010 Málaga, SpainDepartamento de Microbiología y Protección de Cultivos, Instituto de Hortofruticultura Subtropical y Mediterránea «La Mayora», Extensión Campus de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), E-29010 Málaga, SpainDepartamento de Microbiología y Protección de Cultivos, Instituto de Hortofruticultura Subtropical y Mediterránea «La Mayora», Extensión Campus de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), E-29010 Málaga, SpainDepartamento de Microbiología y Protección de Cultivos, Instituto de Hortofruticultura Subtropical y Mediterránea «La Mayora», Extensión Campus de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), E-29010 Málaga, SpainÁrea de Genética, Facultad de Ciencias, Campus Teatinos, Universidad de Málaga, E-29010 Málaga, SpainThe rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 exhibits antagonism toward the phytopathogenic fungus <i>Rosellinia necatrix</i>. This strain efficiently colonizes <i>R. necatrix</i> hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of <i>P. alcaligenes</i> AVO110. The phylogeny of all available <i>P. alcaligenes</i> genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with <i>Pseudomonas otitidis</i>. Core and pan-genome analyses showed that <i>P. alcaligenes</i> strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by <i>R. necatrix</i> exudates. One of these genes (<i>cmpA</i>) encodes a GGDEF/EAL domain protein specific to <i>Pseudomonas</i> spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of <i>P. alcaligenes</i>, including the mycophagous behavior of strain AVO110.https://www.mdpi.com/2076-2607/9/7/1388<i>Pseudomonas alcaligenes</i>mycophagy<i>Rosellinia necatrix</i>fungal exudatesc-di-GMPbiofilm formation |
| spellingShingle | Adrián Pintado Isabel Pérez-Martínez Isabel M. Aragón José Antonio Gutiérrez-Barranquero Antonio de Vicente Francisco M. Cazorla Cayo Ramos The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates Microorganisms <i>Pseudomonas alcaligenes</i> mycophagy <i>Rosellinia necatrix</i> fungal exudates c-di-GMP biofilm formation |
| title | The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates |
| title_full | The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates |
| title_fullStr | The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates |
| title_full_unstemmed | The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates |
| title_short | The Rhizobacterium <i>Pseudomonas alcaligenes</i> AVO110 Induces the Expression of Biofilm-Related Genes in Response to <i>Rosellinia necatrix</i> Exudates |
| title_sort | rhizobacterium i pseudomonas alcaligenes i avo110 induces the expression of biofilm related genes in response to i rosellinia necatrix i exudates |
| topic | <i>Pseudomonas alcaligenes</i> mycophagy <i>Rosellinia necatrix</i> fungal exudates c-di-GMP biofilm formation |
| url | https://www.mdpi.com/2076-2607/9/7/1388 |
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