Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study
Severe Early Childhood Caries (sECC) is a multifactorial disease associated with the occurrence of specific oral microorganisms and other environmental, behavioral, and genetic factors. This study aimed to construct a multivariable model including the occurrence of Candida spp. and selected behavior...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.943480/full |
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| author | Michaela Cvanova Michaela Cvanova Filip Ruzicka Martina Kukletova Bretislav Lipovy Daniela Gachova Lydie Izakovicova Holla Zdenek Danek Zdenek Danek Veronika Hola Michaela Bartosova Jiri Jarkovsky Ladislav Dusek Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova |
| author_facet | Michaela Cvanova Michaela Cvanova Filip Ruzicka Martina Kukletova Bretislav Lipovy Daniela Gachova Lydie Izakovicova Holla Zdenek Danek Zdenek Danek Veronika Hola Michaela Bartosova Jiri Jarkovsky Ladislav Dusek Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova |
| author_sort | Michaela Cvanova |
| collection | DOAJ |
| description | Severe Early Childhood Caries (sECC) is a multifactorial disease associated with the occurrence of specific oral microorganisms and other environmental, behavioral, and genetic factors. This study aimed to construct a multivariable model including the occurrence of Candida spp. and selected behavioral factors (length of breastfeeding, serving sweet beverages and beginning of brushing child’s teeth) to determine their relationships to the occurrence of sECC.In this case-control study 164 children with sECC and 147 children without dental caries were included. MALDI-TOF MS and multiplex qPCR were used to identify Candida spp. and selected bacteria in dental plaque samples, respectively. A questionnaire on oral hygiene, diet, and children’s health was filled in by the parents.The constructed multivariable logistic regression model showed an independent influence of the microbial and behavioral factors in sECC etiopathogenesis. The occurrence of C. albicans and C. dubliniensis was associated with higher odds of sECC development (odds ratio, OR: 9.62 and 16.93, respectively), together with breastfeeding of 6 months or less (OR: 2.71), exposure to sweet beverages (OR: 3.77), and starting to brush child’s teeth after the 12th month of age (OR: 4.10), all statistically significant (p < 0.01).Considering the high occurrence of C. albicans and C. dubliniensis in dental plaque in children with sECC, we propose them as “keystone pathogens” and risk factors for sECC. The models showed that presence of specific species of Candida in dental plaque may be a better descriptor of sECC than the mentioned behavioral factors. |
| first_indexed | 2024-12-11T23:49:32Z |
| format | Article |
| id | doaj.art-b9c2f3e9e5d24df4badfa1d4604cc1ee |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T23:49:32Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b9c2f3e9e5d24df4badfa1d4604cc1ee2022-12-22T00:45:32ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-07-011210.3389/fcimb.2022.943480943480Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control studyMichaela Cvanova0Michaela Cvanova1Filip Ruzicka2Martina Kukletova3Bretislav Lipovy4Daniela Gachova5Lydie Izakovicova Holla6Zdenek Danek7Zdenek Danek8Veronika Hola9Michaela Bartosova10Jiri Jarkovsky11Ladislav Dusek12Petra Borilova Linhartova13Petra Borilova Linhartova14Petra Borilova Linhartova15Petra Borilova Linhartova16RECETOX, Faculty of Science, Masaryk University Kotlarska 2, Brno, CzechiaInstitute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Microbiology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Stomatology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Burns and Plastic Surgery, Institution shared with University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, CzechiaRECETOX, Faculty of Science, Masaryk University Kotlarska 2, Brno, CzechiaClinic of Stomatology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaRECETOX, Faculty of Science, Masaryk University Kotlarska 2, Brno, CzechiaClinic of Maxillofacial Surgery, Institution shared with the University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Microbiology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Stomatology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, CzechiaInstitute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, CzechiaRECETOX, Faculty of Science, Masaryk University Kotlarska 2, Brno, CzechiaClinic of Stomatology, Institution Shared with St. Anne´s University Hospital, Faculty of Medicine, Masaryk University, Brno, CzechiaClinic of Maxillofacial Surgery, Institution shared with the University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, CzechiaSevere Early Childhood Caries (sECC) is a multifactorial disease associated with the occurrence of specific oral microorganisms and other environmental, behavioral, and genetic factors. This study aimed to construct a multivariable model including the occurrence of Candida spp. and selected behavioral factors (length of breastfeeding, serving sweet beverages and beginning of brushing child’s teeth) to determine their relationships to the occurrence of sECC.In this case-control study 164 children with sECC and 147 children without dental caries were included. MALDI-TOF MS and multiplex qPCR were used to identify Candida spp. and selected bacteria in dental plaque samples, respectively. A questionnaire on oral hygiene, diet, and children’s health was filled in by the parents.The constructed multivariable logistic regression model showed an independent influence of the microbial and behavioral factors in sECC etiopathogenesis. The occurrence of C. albicans and C. dubliniensis was associated with higher odds of sECC development (odds ratio, OR: 9.62 and 16.93, respectively), together with breastfeeding of 6 months or less (OR: 2.71), exposure to sweet beverages (OR: 3.77), and starting to brush child’s teeth after the 12th month of age (OR: 4.10), all statistically significant (p < 0.01).Considering the high occurrence of C. albicans and C. dubliniensis in dental plaque in children with sECC, we propose them as “keystone pathogens” and risk factors for sECC. The models showed that presence of specific species of Candida in dental plaque may be a better descriptor of sECC than the mentioned behavioral factors.https://www.frontiersin.org/articles/10.3389/fcimb.2022.943480/fullsevere early childhood caries (sECC)Candida sp.Streptococcus mutansbreastfeedingsweet beveragesbrushing of teeth |
| spellingShingle | Michaela Cvanova Michaela Cvanova Filip Ruzicka Martina Kukletova Bretislav Lipovy Daniela Gachova Lydie Izakovicova Holla Zdenek Danek Zdenek Danek Veronika Hola Michaela Bartosova Jiri Jarkovsky Ladislav Dusek Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova Petra Borilova Linhartova Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study Frontiers in Cellular and Infection Microbiology severe early childhood caries (sECC) Candida sp. Streptococcus mutans breastfeeding sweet beverages brushing of teeth |
| title | Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study |
| title_full | Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study |
| title_fullStr | Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study |
| title_full_unstemmed | Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study |
| title_short | Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study |
| title_sort | candida species and selected behavioral factors co associated with severe early childhood caries case control study |
| topic | severe early childhood caries (sECC) Candida sp. Streptococcus mutans breastfeeding sweet beverages brushing of teeth |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.943480/full |
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