| Summary: | There have been concerns about antibiotics residues in animal products in Nigeria in recent times, but there is no adequate data to support this stance, most especially in south-western Nigeria. Subsequently, this study was carried out to examine the level of tetracycline, doxycycline, chlortetracycline, and oxytetracycline in catfish and chicken from six farms and markets in some parts of Lagos and Ota south-western Nigeria. Eighteen (18) catfish and eighteen (18) white leghorn chickens were obtained and sacrificed. Their body muscles were extracted with McIlvaine buffer-EDTA solution and cleanup with SPE cartridge and analysed with HPLC-DAD at 365 nm. Tetracycline in catfish ranged from ND – 0.0167 µg/g, and in chicken from ND – 0.058 µg/g. Doxycycline in catfish was below the limit of detection for all catfish samples and in chicken ranged from ND – 0.047 µg/g. Chlortetracycline in catfish was between ND – 0.147 µg/g and in chicken from ND – 0.058 µg/g. Oxytetracycline in catfish ranged from ND – 0.021 µg/g and in chicken ND – 0.031 µg/g. Chlortetracycline was higher in one of the catfish samples than the EU recommended maximum residue limits in animal muscles. This research established the administration of these antibiotics in the fishery and poultry farms, and markets examined in Lagos and Ota and the consequence of antibiotics residues in the catfish and chicken samples examined.
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