Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice
Objective To observe the effect of clemastine fumarate(CLE) on intestinal ischemia-reperfusion(I/R) injury in Balb/c mouse models and its correlation with the activation of intestinal NOD-like receptor protein 3(NLRP3) inflammasomes. Methods Balb/c mice were randomly divided into sham operation grou...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1381.pdf |
| _version_ | 1797365878290907136 |
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| author | LIU Yang, LU Ya-peng, HAO Wei, ZHONG Hai-lian, LIU Jie-ting, WANG Ying-bin |
| author_facet | LIU Yang, LU Ya-peng, HAO Wei, ZHONG Hai-lian, LIU Jie-ting, WANG Ying-bin |
| author_sort | LIU Yang, LU Ya-peng, HAO Wei, ZHONG Hai-lian, LIU Jie-ting, WANG Ying-bin |
| collection | DOAJ |
| description | Objective To observe the effect of clemastine fumarate(CLE) on intestinal ischemia-reperfusion(I/R) injury in Balb/c mouse models and its correlation with the activation of intestinal NOD-like receptor protein 3(NLRP3) inflammasomes. Methods Balb/c mice were randomly divided into sham operation group, I/R group (established superior mesenteric artery branch clipping model, ischemia 40 min, reperfusion 2 h) and CLE group (intraperitoneal injection of CLE 5 mg/kg, 1 h before surgery). The pathological changes of intestinal mucosa were evaluated by Chiu's score; The serum levels of TNF-α, IL-1β and IL-18 were detected by ELISA; Western blot was used to detect the expressions of NLRP3, caspase-1 and Gasdermin D proteins in intestinal tissue. Results Compared with the sham group, the Chiu's score in the I/R group significantly increased (P＜0.05). The level of TNF-α, IL-1β and IL-18 in serum was increased (P＜0.05). The expression of NLRP3, caspase-1 and gasdermin D(GSDMD) proteins in intestinal tissue was increased (P＜0.05); Compared with the I/R group, the change of the above indexes from CLE group was alleviated(P＜0.05). Conclusions CLE alleviates intestinal ischemia-reperfusion injury of mouse models and the mechanism may be related to regulation of NLRP3 inflammasome activation. |
| first_indexed | 2024-03-08T16:55:14Z |
| format | Article |
| id | doaj.art-b9c66b2524b54ff9a0f49ae44da5ae84 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:14Z |
| publishDate | 2022-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-b9c66b2524b54ff9a0f49ae44da5ae842024-01-05T02:36:18ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-09-014291381138410.16352/j.issn.1001-6325.2022.09.1381Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in miceLIU Yang, LU Ya-peng, HAO Wei, ZHONG Hai-lian, LIU Jie-ting, WANG Ying-bin0Department of Anesthesiology, Lanzhou University Second Hospital, the Second Clinical Medical School, Lanzhou University, Lanzhou 730030, ChinaObjective To observe the effect of clemastine fumarate(CLE) on intestinal ischemia-reperfusion(I/R) injury in Balb/c mouse models and its correlation with the activation of intestinal NOD-like receptor protein 3(NLRP3) inflammasomes. Methods Balb/c mice were randomly divided into sham operation group, I/R group (established superior mesenteric artery branch clipping model, ischemia 40 min, reperfusion 2 h) and CLE group (intraperitoneal injection of CLE 5 mg/kg, 1 h before surgery). The pathological changes of intestinal mucosa were evaluated by Chiu's score; The serum levels of TNF-α, IL-1β and IL-18 were detected by ELISA; Western blot was used to detect the expressions of NLRP3, caspase-1 and Gasdermin D proteins in intestinal tissue. Results Compared with the sham group, the Chiu's score in the I/R group significantly increased (P＜0.05). The level of TNF-α, IL-1β and IL-18 in serum was increased (P＜0.05). The expression of NLRP3, caspase-1 and gasdermin D(GSDMD) proteins in intestinal tissue was increased (P＜0.05); Compared with the I/R group, the change of the above indexes from CLE group was alleviated(P＜0.05). Conclusions CLE alleviates intestinal ischemia-reperfusion injury of mouse models and the mechanism may be related to regulation of NLRP3 inflammasome activation.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1381.pdfclemastine fumarate| reperfusion injury| intestine| nod-like receptor protein 3(nlrp3)| inflammasome |
| spellingShingle | LIU Yang, LU Ya-peng, HAO Wei, ZHONG Hai-lian, LIU Jie-ting, WANG Ying-bin Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice Jichu yixue yu linchuang clemastine fumarate| reperfusion injury| intestine| nod-like receptor protein 3(nlrp3)| inflammasome |
| title | Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice |
| title_full | Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice |
| title_fullStr | Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice |
| title_full_unstemmed | Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice |
| title_short | Clemastine fumarate attenuates intestinal ischemia-reperfusion injury in mice |
| title_sort | clemastine fumarate attenuates intestinal ischemia reperfusion injury in mice |
| topic | clemastine fumarate| reperfusion injury| intestine| nod-like receptor protein 3(nlrp3)| inflammasome |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1381.pdf |
| work_keys_str_mv | AT liuyangluyapenghaoweizhonghailianliujietingwangyingbin clemastinefumarateattenuatesintestinalischemiareperfusioninjuryinmice |