A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model
Bone critical-size defects and non-union fractures have no intrinsic capacity for self-healing. In this context, the emergence of bone engineering has allowed the development of functional alternatives. The aim of this study was to evaluate the capacity of ASC spheroids in bone regeneration using a...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/12/555 |
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| author | Gabriela S. Kronemberger Thiago Nunes Palhares Alexandre Malta Rossi Brunno R. F. Verçosa Suelen C. Sartoretto Rodrigo Resende Marcelo J. Uzeda Adriana T. N. N. Alves Gutemberg G. Alves Mônica D. Calasans-Maia José Mauro Granjeiro Leandra Santos Baptista |
| author_facet | Gabriela S. Kronemberger Thiago Nunes Palhares Alexandre Malta Rossi Brunno R. F. Verçosa Suelen C. Sartoretto Rodrigo Resende Marcelo J. Uzeda Adriana T. N. N. Alves Gutemberg G. Alves Mônica D. Calasans-Maia José Mauro Granjeiro Leandra Santos Baptista |
| author_sort | Gabriela S. Kronemberger |
| collection | DOAJ |
| description | Bone critical-size defects and non-union fractures have no intrinsic capacity for self-healing. In this context, the emergence of bone engineering has allowed the development of functional alternatives. The aim of this study was to evaluate the capacity of ASC spheroids in bone regeneration using a synergic strategy with 3D-printed scaffolds made from poly (lactic acid) (PLA) and nanostructured hydroxyapatite doped with carbonate ions (CHA) in a rat model of cranial critical-size defect. In summary, a set of results suggests that ASC spheroidal constructs promoted bone regeneration. In vitro results showed that ASC spheroids were able to spread and interact with the 3D-printed scaffold, synthesizing crucial growth factors and cytokines for bone regeneration, such as VEGF. Histological results after 3 and 6 months of implantation showed the formation of new bone tissue in the PLA/CHA scaffolds that were seeded with ASC spheroids. In conclusion, the presence of ASC spheroids in the PLA/CHA 3D-printed scaffolds seems to successfully promote bone formation, which can be crucial for a significant clinical improvement in critical bone defect regeneration. |
| first_indexed | 2024-03-08T20:39:10Z |
| format | Article |
| id | doaj.art-b9c7fd3f2d9c4b2c94b14994ffd2cd44 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-08T20:39:10Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-b9c7fd3f2d9c4b2c94b14994ffd2cd442023-12-22T14:17:58ZengMDPI AGJournal of Functional Biomaterials2079-49832023-11-01141255510.3390/jfb14120555A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect ModelGabriela S. Kronemberger0Thiago Nunes Palhares1Alexandre Malta Rossi2Brunno R. F. Verçosa3Suelen C. Sartoretto4Rodrigo Resende5Marcelo J. Uzeda6Adriana T. N. N. Alves7Gutemberg G. Alves8Mônica D. Calasans-Maia9José Mauro Granjeiro10Leandra Santos Baptista11Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, BrazilBrazilian Center for Physics Research, Xavier Sigaud 150, Urca 22290-180, RJ, BrazilBrazilian Center for Physics Research, Xavier Sigaud 150, Urca 22290-180, RJ, BrazilNucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, BrazilLaboratory of Eukariotic Cells, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias 25250-020, RJ, BrazilNucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, BrazilBone critical-size defects and non-union fractures have no intrinsic capacity for self-healing. In this context, the emergence of bone engineering has allowed the development of functional alternatives. The aim of this study was to evaluate the capacity of ASC spheroids in bone regeneration using a synergic strategy with 3D-printed scaffolds made from poly (lactic acid) (PLA) and nanostructured hydroxyapatite doped with carbonate ions (CHA) in a rat model of cranial critical-size defect. In summary, a set of results suggests that ASC spheroidal constructs promoted bone regeneration. In vitro results showed that ASC spheroids were able to spread and interact with the 3D-printed scaffold, synthesizing crucial growth factors and cytokines for bone regeneration, such as VEGF. Histological results after 3 and 6 months of implantation showed the formation of new bone tissue in the PLA/CHA scaffolds that were seeded with ASC spheroids. In conclusion, the presence of ASC spheroids in the PLA/CHA 3D-printed scaffolds seems to successfully promote bone formation, which can be crucial for a significant clinical improvement in critical bone defect regeneration.https://www.mdpi.com/2079-4983/14/12/555boneosteogenesiscritical-size defectstissue engineeringspheroidssynergic strategy |
| spellingShingle | Gabriela S. Kronemberger Thiago Nunes Palhares Alexandre Malta Rossi Brunno R. F. Verçosa Suelen C. Sartoretto Rodrigo Resende Marcelo J. Uzeda Adriana T. N. N. Alves Gutemberg G. Alves Mônica D. Calasans-Maia José Mauro Granjeiro Leandra Santos Baptista A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model Journal of Functional Biomaterials bone osteogenesis critical-size defects tissue engineering spheroids synergic strategy |
| title | A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model |
| title_full | A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model |
| title_fullStr | A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model |
| title_full_unstemmed | A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model |
| title_short | A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model |
| title_sort | synergic strategy adipose derived stem cell spheroids seeded on 3d printed pla cha scaffolds implanted in a bone critical size defect model |
| topic | bone osteogenesis critical-size defects tissue engineering spheroids synergic strategy |
| url | https://www.mdpi.com/2079-4983/14/12/555 |
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