Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes
In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2015-03-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/312 |
| _version_ | 1797922408997322752 |
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| author | Celal Türker Bahri Devrim Özcan |
| author_facet | Celal Türker Bahri Devrim Özcan |
| author_sort | Celal Türker |
| collection | DOAJ |
| description | In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis. |
| first_indexed | 2024-04-10T14:30:55Z |
| format | Article |
| id | doaj.art-b9caac12d26f493da0539aded0363fd7 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T14:30:55Z |
| publishDate | 2015-03-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-b9caac12d26f493da0539aded0363fd72023-02-15T16:08:50ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2015-03-0136148Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the EnzymesCelal Türker0Bahri Devrim Özcan1Osmaniye Korkut Ata UniversityOsmaniye Korkut Ata UniversityIn the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis.http://www.agrifoodscience.com/index.php/TURJAF/article/view/312Alpha-amylaseBacillus sp.IsolationCharacterizationThermophil |
| spellingShingle | Celal Türker Bahri Devrim Özcan Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes Turkish Journal of Agriculture: Food Science and Technology Alpha-amylase Bacillus sp. Isolation Characterization Thermophil |
| title | Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes |
| title_full | Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes |
| title_fullStr | Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes |
| title_full_unstemmed | Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes |
| title_short | Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes |
| title_sort | isolation of alpha amylase producing thermophilic bacillus strains and partial characterization of the enzymes |
| topic | Alpha-amylase Bacillus sp. Isolation Characterization Thermophil |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/312 |
| work_keys_str_mv | AT celalturker isolationofalphaamylaseproducingthermophilicbacillusstrainsandpartialcharacterizationoftheenzymes AT bahridevrimozcan isolationofalphaamylaseproducingthermophilicbacillusstrainsandpartialcharacterizationoftheenzymes |