Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines
Background: Among the secondary metabolites isolated from Allium species, steroidal saponins are more important for their cytotoxic activities on a variety of cancer cell lines. Current study has investigated the cytotoxic activity of saponin-riched butanolic fraction of Allium affine, an important...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2017-12-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-1660-en.html |
| _version_ | 1830508710262210560 |
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| author | Maliheh Kazemi Behzad Zolfaghari Mahammad Keyvanlo Shahrestanaki Masoud Sadeghi Dinani |
| author_facet | Maliheh Kazemi Behzad Zolfaghari Mahammad Keyvanlo Shahrestanaki Masoud Sadeghi Dinani |
| author_sort | Maliheh Kazemi |
| collection | DOAJ |
| description | Background: Among the secondary metabolites isolated from Allium species, steroidal saponins are more important for their cytotoxic activities on a variety of cancer cell lines. Current study has investigated the cytotoxic activity of saponin-riched butanolic fraction of Allium affine, an important edible Allium species of Iran.
Objective: This study was conducted to evaluate cytotoxic activity of saponin-riched fraction of Allium affine Ledeb on breast and ovarian cancer cell lines.
Methods: Bulbs of the plant were extracted respectively by hexane, chloroform, chloroform-methanol (9-1) and methanol in a stepwise method with increasing solvent polarity. The methanol extract was then partitioned between water and butanol and the final dried butanolic fraction was used for further cytotoxicity assay. MCF-7, MDA-MB231 and OVCAR-3 cell lines were tested for cytotoxic activity, using different concentrations of butanolic fraction (0.1-200 µg/ml) by MTT assay. After the incubation time (48 h), the percentage of the viable cells was determined by ELISA reader instrument in 570 nm.
Results: All of the cell lines tested in this investigation exhibited a dose-dependent decrease in succinate dehydrogenase activity against the control and significant dose-dependent inhibition of the growth in all concentrations and cell lines were observed (P<0.01). The most cytotoxic activity was observed for OVCAR-3 cell line (IC50= 7.13±0.94 µg/ml).
Conclusion: The butanolic fraction of A. affine exhibited a significant cytotoxic activity on investigated cell lines, especially OVCAR-3, which is in agreement with other studies conducted on different Allium species. According to the results, it seems that the butanolic fraction of this plant contains cytotoxic components, especially steroidal saponins, and the phytochemical study of the constituents is suggested. |
| first_indexed | 2024-12-22T01:22:58Z |
| format | Article |
| id | doaj.art-b9cafb844c664efe87afe15bbdda0539 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T01:22:58Z |
| publishDate | 2017-12-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-b9cafb844c664efe87afe15bbdda05392022-12-21T18:43:42ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582017-12-0116648390Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell LinesMaliheh Kazemi0Behzad Zolfaghari1Mahammad Keyvanlo Shahrestanaki2Masoud Sadeghi Dinani3 School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Department of clinical Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran Background: Among the secondary metabolites isolated from Allium species, steroidal saponins are more important for their cytotoxic activities on a variety of cancer cell lines. Current study has investigated the cytotoxic activity of saponin-riched butanolic fraction of Allium affine, an important edible Allium species of Iran. Objective: This study was conducted to evaluate cytotoxic activity of saponin-riched fraction of Allium affine Ledeb on breast and ovarian cancer cell lines. Methods: Bulbs of the plant were extracted respectively by hexane, chloroform, chloroform-methanol (9-1) and methanol in a stepwise method with increasing solvent polarity. The methanol extract was then partitioned between water and butanol and the final dried butanolic fraction was used for further cytotoxicity assay. MCF-7, MDA-MB231 and OVCAR-3 cell lines were tested for cytotoxic activity, using different concentrations of butanolic fraction (0.1-200 µg/ml) by MTT assay. After the incubation time (48 h), the percentage of the viable cells was determined by ELISA reader instrument in 570 nm. Results: All of the cell lines tested in this investigation exhibited a dose-dependent decrease in succinate dehydrogenase activity against the control and significant dose-dependent inhibition of the growth in all concentrations and cell lines were observed (P<0.01). The most cytotoxic activity was observed for OVCAR-3 cell line (IC50= 7.13±0.94 µg/ml). Conclusion: The butanolic fraction of A. affine exhibited a significant cytotoxic activity on investigated cell lines, especially OVCAR-3, which is in agreement with other studies conducted on different Allium species. According to the results, it seems that the butanolic fraction of this plant contains cytotoxic components, especially steroidal saponins, and the phytochemical study of the constituents is suggested.http://jmp.ir/article-1-1660-en.html<i>allium affine</i>breast cancercytotoxicityovarian cancersteroidal saponin |
| spellingShingle | Maliheh Kazemi Behzad Zolfaghari Mahammad Keyvanlo Shahrestanaki Masoud Sadeghi Dinani Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines Journal of Medicinal Plants <i>allium affine</i> breast cancer cytotoxicity ovarian cancer steroidal saponin |
| title | Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines |
| title_full | Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines |
| title_fullStr | Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines |
| title_full_unstemmed | Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines |
| title_short | Cytotoxic Effects of Allium affine Ledeb Butanolic Fraction on Breast and Ovary Cancer Cell Lines |
| title_sort | cytotoxic effects of allium affine ledeb butanolic fraction on breast and ovary cancer cell lines |
| topic | <i>allium affine</i> breast cancer cytotoxicity ovarian cancer steroidal saponin |
| url | http://jmp.ir/article-1-1660-en.html |
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